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VAMP-8 is a membrane protein that is a member of the SNARE protein family. It is involved in the fusion of vesicles with the plasma membrane, facilitating the release of their contents.

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Lab products found in correlation

Mg132, by Merck Group (1 mentions) U46619, by Cayman Chemical (1 mentions) Stir bars, by Chrono-Log (1 mentions) Ubiquitin, by Santa Cruz Biotechnology (1 mentions) Fitc conjugated annexin 5, by Cell Signaling Technology (1 mentions) Anti p selectin, by Cell Signaling Technology (1 mentions) Pac 1, by Cell Signaling Technology (1 mentions) Bcl10, by Santa Cruz Biotechnology (1 mentions) Snap 23, by Santa Cruz Biotechnology (1 mentions) Thrombin, by Chrono-Log (1 mentions)

2 protocols using vamp 8

1

Platelet Activation and Signaling Pathways

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ADP, MG132 were obtained from Sigma Aldrich (St. Louis, MO). Thrombin, Collagen, stir bars and other disposables were from Chrono-Log (Havertown, PA), and U46619 was obtained from Cayman Chemical (Ann Arbor, MI). The proteasome inhibitor bortezomib (Velcade) was generous gift from Dr. Steven Schwarze (University of Kentucky College of Medicine, Lexington, KY). FITC-conjugated Annexin V, anti—P-selectin, and PAC-1 antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA). Antibodies against TAK1, TAB2, CARMA1, MALT1, Bcl10, Ubiquitin, IKKα, IKKβ, IKKγ, VAMP-8, SNAP-23, Syn-11, and pSer/Thr/Tyr from Santa Cruz (Santa Cruz, CA), respectively. Other reagents were of analytical grade.
Karim Z.A., Vemana H.P, & Khasawneh F.T. (2015). MALT1-Ubiquitination Triggers Non-Genomic NF-κB/IKK Signaling upon Platelet Activation. PLoS ONE, 10(3), e0119363.
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2

Vesicle Isolation and Characterization

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The entire vesicle preparation was divided into four parts, with one part exposed to protein A-sepharose beads and the other three fractions subjected to protein A-sepharose-conjugated VAMP-3, VAMP-7, or VAMP-8 (Santa Cruz Biotechnology) and incubated for 2 h on ice with intermittent mixing every 30 min. The incubate was centrifuged at 1000g for 1 min, and the pellet containing beads with immunoisolated vesicles was washed three times in 500 μL of PBS pH 7.4. The beads were eluted using 50 μL of PBS pH 3 to release the bound vesicles, centrifuged at 1000g for 1 min to separate the protein A-sepharose-conjugated VAMP antibody, and the pH of the resulting suspension was raised to 7.0 in a total volume of 350 μL for both AFM and mass spectrometry.
Jena B.P., Stemmer P.M., Wang S., Mao G., Lewis K.T, & Walz D.A. (2017). Human Platelet Vesicles Exhibit Distinct Size and Proteome. Journal of proteome research, 16(7), 2333-2338.
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