The largest database of trusted experimental protocols

5 protocols using cholesterol

1

Ethosomes Vesicle Development Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tocopherol acetate and propylene glycol was purchased from Sigma-Aldrich Chemie GmbH (Taufkirchen, Germany); Cholesterol was obtained from AppliChem GmbH (Darmstadt, Germany); Soy phosphatidylcholine 90 G were obtained from Lipoid GmbH (Ludwigshafen, Germany). Carbopol-940 and Ethanol by Merck KGaA (Gernsheim, Germany). All the chemicals and materials used for ethosomes vesical development were of analytical standard. Double distilled water (ddH2O) was used throughout the study.
+ Open protocol
+ Expand
2

Formulation and Characterization of Lipid Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols
EM was procured from BASF, Germany. Phospholipon 90G was obtained as gift sample from Phospholipid GmbH (Nattermannallee, Germany). SDC and cholesterol were bought from AppliChem Panreac, Darmstadt, Germany. SP was obtained from Fluka Chemica (Buch, Switzerland). Rhodamine 6G was sourced from Sigma-Aldrich (St. Louis, MO, USA). Methanol and chloroform were purchased from BDH, England, and Sigma-Aldrich, USA, respectively.
+ Open protocol
+ Expand
3

Sterol Biosynthesis Pathway Optimization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cholesterol (cholest-5-en-3β-ol) was obtained from AppliChem (Germany); phytosterol (total sterols content—95.47%: β-sitosterol—42.39%, stigmasterol—26.08%, campesterol—23.48%, brassicasterol—3.52%)—from Jiangsu Spring Fruit Biological Products Co., Ltd. (China); pregn-4-ene-3,20-dione (progesterone); 3β-hydroxypregn-5-en-20-one (pregnenolone); 3β-hydroxyandrost-5-en-17-one (dehydroepiandrosterone, DHEA); acetamide—from Sigma-Aldrich (USA); yeast extract, bacto-peptone—from Difco (USA); randomly methylated β-cyclodextrin (MCD)—from Wacker-Chemie GmbH (Germany). Hygromycin B and DNA modifying enzymes were obtained from Thermo Fisher Scientific (USA); Tween 80—from Serva (Germany); DNA purification Kits—from Qiagen (Germany). The DNA manipulations were carried out according to the manufacturer’s instructions. All other reagents were of the highest purity grade and were purchased from domestic commercial suppliers (Russia).
+ Open protocol
+ Expand
4

Diacerein-Based Topical Formulation Development

Check if the same lab product or an alternative is used in the 5 most similar protocols
Diacerein was received as a research material donation from the Global Pharmaceuticals (Pvt) Ltd., Islamabad, Pakistan. Egg lecithin was purchased from Rongsheng Bio-Tech Co., Ltd., Shanghai, China. Soy lecithin was received as a gift from Lipoid, Switzerland, and cholesterol was purchased from AppliChem, Darmstadt Germany. Carbopol-934, maltodextrin (DE 16.5–19.5), triethanolamine, methanol and chloroform of HPLC grade were purchased from Sigma Aldrich, Chemie GmbH, Germany.
+ Open protocol
+ Expand
5

Antibacterial Formulations Optimization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ertapenem injectable powder (INVANZ®) manufactured by Merck Sharp & Dohme Corp., INC., Kenilworth, NJ., USA, Meropenem injectable powder (MERONEM®) manufactured by Pfizer Limited, NJ, UK and Tigecycline injectable powder (TYGACIL®) manufactured by Wyeth Lederle S.p.A, CT, Italy were purchased and used in this study. Sorbitan monostearate (Span 60) and lecithin were purchased from Sigma Chemical Co., St Louis, MO, USA. Octadecyl-amine (stearyl amine) was ordered from Sigma-Aldrich CHEMI., GmbH., Steinheim., Germany. Absolute ethanol was obtained from Fisher BioReagents, Loughborough, Leicestershire, UK. Cholesterol was purchased from AppliChem, Darmstadt, Germany. Mueller-Hinton (MH) agar and broth, tryptic Soy (TS) broth, Luria Bertani (LB) broth and MacConkey’s agar were all purchased from LAB A Neogen Company, Bury, UK. All other reactants, solvents and media were of analytical grade or higher.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!