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Dneasy 96 blood tissue plate kit

Manufactured by Qiagen
Sourced in Netherlands

The DNeasy® 96 Blood & Tissue plate kit is a product designed for the purification of genomic DNA from a variety of biological samples, including blood, cultured cells, and tissue samples. It utilizes a silica-based membrane technology to efficiently capture and purify DNA, making it suitable for downstream applications such as PCR, sequencing, and other molecular biology techniques.

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2 protocols using dneasy 96 blood tissue plate kit

1

Skin Biopsy DNA Extraction

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Sixteen or 24 skin biopsies (ø 0.6 cm) were taken from each skin as previously described (11 (link)). All genomic DNA (gDNA) extractions were performed with either the DNeasy® Blood & Tissue spinning column kit (Qiagen, Venlo, Netherlands) or the DNeasy® 96 Blood & Tissue plate kit (Qiagen) according to supplier’s protocol.
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2

Genomic DNA Extraction from Mouse Skin and Sand Flies

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All genomic DNA (gDNA) extractions were performed with either the DNeasy Blood & Tissue spinning column kit (Qiagen) or the DNeasy 96 Blood & Tissue plate kit (Qiagen) according to supplier’s protocol. Per mouse, 100 µl of blood and 24 punch biopsies (ø 0.8 cm) were collected from the body skin (excluding paws, snout and ears) in a regular pattern (Fig. 1b) for gDNA extraction. Per fly exposure, 40 fed sand flies were collected per mouse for gDNA extraction.
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