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Nuclease p1 np1 from penicillium citrinum

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Nuclease P1 (NP1) is an enzyme purified from the fungus Penicillium citrinum. It exhibits non-specific endonuclease activity, capable of cleaving single-stranded and double-stranded DNA and RNA.

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Lab products found in correlation

Oligodeoxynucleotides, by Integrated DNA Technologies (2 mentions) Hplc solvents, by Thermo Fisher Scientific (2 mentions) Oligodeoxynucleotides odns, by Integrated DNA Technologies (1 mentions) High performance liquid chromatography solvents, by Thermo Fisher Scientific (1 mentions) System gold hplc system, by Beckman Coulter (1 mentions) Cary 100 bio uv vis spectrometer, by Agilent Technologies (1 mentions) Model 168, by Beckman Coulter (1 mentions) J 810 spectropolarimeter, by Jasco (1 mentions)

Close spelling variants of this product

Nuclease P1 from Penicillium citrinum Nuclease P1 (penicillium citrinum, Nuclease P1 from Nuclease P1

3 protocols using nuclease p1 np1 from penicillium citrinum

1

Oligodeoxynucleotide Characterization Protocol

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Oligodeoxynucleotides were obtained from Integrated DNA Technologies, Inc. (IDT) (Coralville, Iowa) and Nuclease P1 (NP1) from Penicillium citrinum was from Sigma (St. Louis, MO). Ammonium citrate and 3-hydroxypicolinic acid (3-HPA) MALDI matrices were purchased from Fluka (Milwaukee, WI). HPLC solvents were from Fisher (Fair Lawn, NJ).
Smith-Carpenter J.E, & Taylor J.S. (2018). Photocrosslinking of G-Quadruplex Forming Sequences found in Human Promoters. Photochemistry and photobiology, 95(1), 252-266.
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2

Oligodeoxynucleotide Analysis by MALDI-TOF MS

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Oligodeoxynucleotides (ODNs) were purchased from Integrated DNA Technologies, Inc. (IDT) (Coralville, IA, USA). Nuclease P1 (NP1) from Penicillium citrinum was obtained from Sigma (St. Louis, MO, USA). Ammonium citrate and 3-hydroxypicolinic acid for use as matrix assisted laser desorption ionization (MALDI) matrices were purchased from Fluka (Milwaukee, WI, USA). High-performance liquid chromatography (HPLC) solvents were from Fisher (Fair Lawn, NJ, USA).
Smith J.E., Lu C, & Taylor J.S. (2014). Effect of sequence and metal ions on UVB-induced anti cyclobutane pyrimidine dimer formation in human telomeric DNA sequences. Nucleic Acids Research, 42(8), 5007-5019.
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3

HPLC Analysis of Oligodeoxynucleotide Modifications

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Oligodeoxynucleotides were purchased from Integrated DNA Technologies, Inc. (IDT) (Coralville, Iowa). Nuclease P1 (NP1) from Penicillium citrinum, was from Sigma (St. Louis, MO). HPLC solvents were from Fisher (Fair Lawn, NJ). HPLC separation and analysis were carried out on System Gold HPLC system with a binary gradient Model 125 pump and a Model 168 diode array detector (Beckman Coulter, Inc., Fullerton, CA). An X-Bridge column (C18, 4.6 × 75 mm, 2.5 μm, 135 Å) from Waters Corporation (Milford, MA) was used for reverse-phase HPLC. UVB (280-320 nm) irradiation was carried out with two Spectroline XX-15B UV 15W tubes (312 nm) with peak UV intensity of 1150 μW/cm2 at 25 cm filtered through a Longlife filter glass from Spectronics Corporation (Westbury, NY). CD experiments were carried out on a J-810 spectropolarimeter (Jasco). UV melting curves were obtained on a Cary 100 Bio UV-VIS Spectrometer (Varian).
Lu C., Smith-Carpenter J.E, & Taylor J.S. (2018). Evidence for Reverse Hoogsteen Hairpin Intermediates in the Photocrosslinking of Human Telomeric DNA Sequences. Photochemistry and photobiology, 94(4), 685-697.
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