Log In Sign up
The largest database of trusted experimental protocols

P47phox a 7

Manufactured by Santa Cruz Biotechnology
Visit Supplier

P47phox (A-7) is a primary antibody that specifically recognizes the p47phox (NCF1) protein. p47phox is a key component of the NADPH oxidase complex, which is responsible for the production of superoxide radicals in phagocytic cells.

Automatically generated - may contain errors

Lab products found in correlation

Phalloidin ifluor 405, by Abcam (2 mentions) Anti mouse cd16 32 2.4g2, by BD (2 mentions)

Spelling variants of this product

P47phox (47 citations)

2 protocols using p47phox a 7

1

Neutrophil Localization of EphA2 and p47phox

Check if the same lab product or an alternative is used in the 5 most similar protocols
To determine protein localization within the neutrophils, the cells were incubated in RPMI 1640 medium containing 2% heat-inactivated mouse serum for 30 min on fibronectin coated coverslips. The cells were then incubated with opsonized C. albicans CAI4-GFP for 30 min. Next, they were fixed with 3% paraformaldehyde, blocked with 10% BSA, followed by anti-mouse CD16/32 (2.4G2; BD Biosciences), and incubated with antibodies against EphA2 (D4A2, #6997; Cell Signaling), and p47phox (A-7; Santa Cruz), followed by an Alexa 488-conjugated mouse anti-rabbit antibody. To visualize F-actin, the cells were also stained with Phalloidin-IFluor 405 (Abcam). The cells were then imaged by confocal microscopy.
Swidergall M., Solis N.V., Wang Z., Phan Q.T., Marshall M.E., Lionakis M.S., Pearlman E, & Filler S.G. (2019). EphA2 Is a Neutrophil Receptor for Candida albicans that Stimulates Antifungal Activity during Oropharyngeal Infection. Cell reports, 28(2), 423-433.e5.
+ Open protocol
+ Expand
2

Neutrophil Localization of EphA2 and p47phox

Check if the same lab product or an alternative is used in the 5 most similar protocols
To determine protein localization within the neutrophils, the cells were incubated in RPMI 1640 medium containing 2% heat-inactivated mouse serum for 30 min on fibronectin coated coverslips. The cells were then incubated with opsonized C. albicans CAI4-GFP for 30 min. Next, they were fixed with 3% paraformaldehyde, blocked with 10% BSA, followed by anti-mouse CD16/32 (2.4G2; BD Biosciences), and incubated with antibodies against EphA2 (D4A2, #6997; Cell Signaling), and p47phox (A-7; Santa Cruz), followed by an Alexa 488-conjugated mouse anti-rabbit antibody. To visualize F-actin, the cells were also stained with Phalloidin-IFluor 405 (Abcam). The cells were then imaged by confocal microscopy.
Swidergall M., Solis N.V., Wang Z., Phan Q.T., Marshall M.E., Lionakis M.S., Pearlman E, & Filler S.G. (2019). EphA2 Is a Neutrophil Receptor for Candida albicans that Stimulates Antifungal Activity during Oropharyngeal Infection. Cell reports, 28(2), 423-433.e5.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!