Incubation in anti-Phosphorylated tau (1:500, monoclonal, mouse, Thermo Scientific) with anti-NeuN (1:1000) overnight was followed by secondary antibodies, anti-mouse-alexa-488 (1:1000) and anti-rabbit-alexa-594 (1:500) respectively, for 6h. Sections were rinsed and mounted onto slides.
Anti phosphorylated tau
Manufactured by Thermo Fisher Scientific
Sourced in United States
Anti-phosphorylated tau is a laboratory reagent used to detect and quantify the presence of phosphorylated tau protein in biological samples. It serves as a tool for researchers to study the mechanisms of neurodegenerative diseases.
Automatically generated - may contain errors
Lab products found in correlation
Anti lc3b, by Merck Group (1 mentions)
Anti lamp2, by Abcam (1 mentions)
Anti calregulin, by Santa Cruz Biotechnology (1 mentions)
Anti tom20, by Santa Cruz Biotechnology (1 mentions)
Anti actin, by Abcam (1 mentions)
Anti tau, by Abcam (1 mentions)
Anti calbindin, by Merck Group (1 mentions)
Anti neun, by Merck Group (1 mentions)
Anti gapdh, by Abcam (1 mentions)
3 protocols using anti phosphorylated tau
1
Quantifying Phosphorylated Tau in Neurons
2
Immunostaining of Phosphorylated Tau
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A third series was incubated in anti-phosphorylated tau (1:500, monoclonal, mouse, Thermo Scientific) with anti-NeuN (1:1000) overnight. Secondary antibodies were anti-mouse-alexa-488 (1:1000) and anti-rabbit-alexa-594 (1:500) respectively, for 6 h. Sections were rinsed and mounted onto slides.
3
Immunohistochemical Antibody Profiling
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Antibodies used as secondary antibodies were anti-Calbindin (1 : 250; Sigma, St. Louis, MO, USA; Cat. No. C2724), anti-LC3B (1 : 500; Sigma, L7543), anti-LAMP2 (1 : 300; Abcam, Cambridge, MA, USA; Cat. No. ab13524), anti-p62 (1 : 200; American Research Products, Waltham, MA, USA; Cat. No. 03-GP62-C), Cy3-conjugated anti-rabbit IgG (1 : 2000; Upstate Biotechnology, New York, NY, USA; Cat. No. 715–095-150), anti-NeuN (1 : 100; Millipore, Billerica, MA, USA; Cat. No. ABN90), anti-tau (1 : 1000; Abcam, Cat. No. ab64193), anti-phosphorylated tau (1 : 200; Thermo Fisher Scientific, Lenexa, KS, USA; Cat. No. AT270), anti-DLP1 (1 : 1000; Abcam, Cat. No.ab156951), anti-actin (1 : 2000; Abcam, Cat. No. AB8277), anti-LRRK2 MJFF4 (1 : 2000; Epitomics, Burlingame, CA, USA; Cat.No. 3516), anti-Tom20 (1 : 250; SantaCruz, Dallas, TX, USA; Cat. No. sc-17764), anti-calregulin (1 : 250; SantaCruz, Cat. No. sc-6468-R), anti-Gapdh (1 : 2500; Abcam, Cat. No. ab9483), anti-Golgi 58 (1 : 5000; Thermo Fisher Scientific, Cat. No. MA1-22144), and Alexa Fluor-conjugated antibodies (1 : 2000; Molecular Probes, Eugene, OR, USA; Cat. No. A21057, A21076, A21096).
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