The following antibodies were used in this study: α-synuclein monoclonal antibody (BD Biosciences; #610787, San Diego, CA), α-synuclein monoclonal antibody #274, phosphorylated α-synuclein polyclonal antibody (Abcam, ab59264; Cambridge, MA), GFP (c-terminus) polyclonal antibody (IMGENEX, #5127A; San Diego, CA), GFP (N-terminus) polyclonal antibody (Cell signaling Technology, #2555; Beverly, MA), GFP (N-terminus) monoclonal antibody (Abcam, ab127417), GCase monoclonal antibody 8E4 (from J. Barranger, University of Pittsburgh), GCase polyclonal antibody (Sigma, G4171; St. Louis, MO), p62 monoclonal antibody (BD Transduction laboratories, #c2384-0B; Swampscott, MA), ubiquitin polyclonal antibodies (Dako; Glostrup, Denmark and Chemicon; Temecula, CA), and β-actin monoclonal antibody (Sigma). Fluorescence dye-conjugated goat anti-rabbit IgG was purchased from Jackson Immunoresearch Laboratories (West Grove, PA). The Q tracker 858 cell labeling kit was purchased from Invitrogen (Carlsbad, CA).
Ubiquitin polyclonal antibodies
Manufactured by Agilent Technologies
Sourced in Morocco, Denmark, United States
Ubiquitin polyclonal antibodies are reagents used in research applications to detect and study ubiquitin, a small regulatory protein found in eukaryotic cells. These antibodies are designed to specifically bind to ubiquitin, enabling its identification and quantification in various experimental techniques.
Automatically generated - may contain errors
Lab products found in correlation
β actin monoclonal antibody, by Merck Group (2 mentions)
Gcase monoclonal antibody 8e4, by Merck Group (2 mentions)
Hrp conjugated goat anti rabbit igg h l, by Bio-Rad (2 mentions)
Fluorescein conjugated dextran, by Thermo Fisher Scientific (2 mentions)
α synuclein monoclonal antibody, by BD (1 mentions)
Ctd 19, by Abcam (1 mentions)
Ab274, by Bio-Rad (1 mentions)
Lysotracker red dnd 99, by Thermo Fisher Scientific (1 mentions)
Ac 15, by Merck Group (1 mentions)
Hrp conjugated goat anti mouse igg h l, by Bio-Rad (1 mentions)
Lysotracker red dnd 99 l 7528, by Thermo Fisher Scientific (1 mentions)
3 protocols using ubiquitin polyclonal antibodies
1
Antibody Panel for Parkinson's Research
2
Antibody Immunoblotting Protocol
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The following antibodies were used in this study: CTSD monoclonal antibody CTD-19 (ab6313; Abcam, Cambridge, MA, USA; 1 : 1000 dilution), β-actin monoclonal antibody AC-15 (A5441; Sigma-Aldrich, St. Louis, MO, USA; 1 : 10 000 dilution), p62 monoclonal antibody p62 LCK ligand (610833; BD Transduction Laboratories, Swampscott, MA, USA; 1 : 1000 dilution), ubiquitin polyclonal antibodies (Dako, Glostrup, Denmark, and Chemicon, Temecula, CA, USA; 1 : 1000 dilution), α-synuclein monoclonal antibody (610787; BD Biosciences, San Diego, CA, USA; 1 : 1500 dilution), α-synuclein monoclonal antibody Ab274 (1 : 1500 dilution), α-synuclein monoclonal antibody Ab62 (1 : 1000 dilution), HRP-conjugated goat anti-mouse IgG (H+L) (172-1011; Bio-Rad Laboratories, Hercules, CA, USA; 1 : 3000 dilution) and HRP-conjugated goat anti-rabbit IgG (H+L) (Bio-Rad Laboratories; 1 : 3,000 dilution).
Fluorescein-conjugated dextran (10 000 molecular weight; D-1821), TO-PRO-3 iodide (T3605) and LysoTracker Red DND-99 (L-7528) were purchased from Invitrogen (Carlsbad, CA, USA).
Fluorescein-conjugated dextran (10 000 molecular weight; D-1821), TO-PRO-3 iodide (T3605) and LysoTracker Red DND-99 (L-7528) were purchased from Invitrogen (Carlsbad, CA, USA).
3
Antibody and Fluorescence Reagent Protocol
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The following antibodies were used in this study: GCase monoclonal antibody 8E4 (from J Barranger, University of Pittsburgh; 1:1000), GCase polyclonal antibody (G4171, Sigma-Aldrich, St Louis, MO, USA; 1:1000), β-actin monoclonal antibody (Sigma-Aldrich; 1:10 000), p62 monoclonal antibody (c2384-0B, BD Transduction Laboratories, Swampscott, MA, USA; 1:1000), ubiquitin polyclonal antibodies (Dako, Glostrup, Denmark and Chemicon, Temecula, CA, USA; 1:1000), α-synuclein monoclonal antibody (610787, BD Biosciences; 1:1500), α-synuclein monoclonal antibody Ab274 (1:1500),17 (link) α-synuclein monoclonal antibody Ab62 (1:1000),17 (link) Horse radish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin G (IgG; H+L) (172–1011, Bio-Rad Laboratories, Hercules, CA, USA; 1:3000) and HRP-conjugated goat anti-rabbit IgG (H+L) (Bio-Rad Laboratories; 1:3000).
Fluorescein-conjugated dextran (10 000 molecular weight; D-1821), TO-PRO-3 iodide (T3605) and LysoTracker Red DND-99 (L-7528) were purchased from Invitrogen (Carlsbad, CA, USA).
Fluorescein-conjugated dextran (10 000 molecular weight; D-1821), TO-PRO-3 iodide (T3605) and LysoTracker Red DND-99 (L-7528) were purchased from Invitrogen (Carlsbad, CA, USA).
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