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Cytometric bead array cba

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The Cytometric Bead Array (CBA) is a multiplex assay system that uses flow cytometry to measure multiple analytes simultaneously in a single sample. The CBA utilizes beads coated with capture antibodies specific to different target proteins, allowing for the quantification of multiple proteins in a sample.

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Lab products found in correlation

Facscanto 2, by BD (7 mentions) Fcap array software, by BD (5 mentions) Elisa, by R&D Systems (5 mentions) Facscan flow cytometer, by BD (4 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (4 mentions) Fcap array software version 3, by BD (3 mentions) Fcap array v3, by BD (3 mentions) Cba software, by BD (3 mentions) Accuri c6 flow cytometer, by BD (3 mentions) Lsrii flow cytometer, by BD (3 mentions) Facsverse bioanalyzer, by BD (2 mentions) Lsrii fortessa, by BD (2 mentions) Facsverse flow cytometer, by BD (2 mentions) Ifn λ1, by Thermo Fisher Scientific (2 mentions) Brefeldin a, by Merck Group (2 mentions) Fcap array software v3, by BD (2 mentions) Losmapimod, by GlaxoSmithKline (2 mentions) Ficoll paque histopaque, by Merck Group (2 mentions) Cellquest pro, by BD (2 mentions) Facscalibur flow cytometer, by BD (2 mentions)

123 protocols using cytometric bead array cba

1

Cytokine Profiling of DC Responses

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Supernatants of DC cultures were harvested 24 h after stimulation with live and inactivated S. aureus, filtered (0.2 μm), and stored at −80°C. The production of IL-12, TNF-α, IL-10, IL-1β, IL-6, and IL-8 was measured by human Inflammatory Cytokine kit (Cytometric Bead Array, CBA, BD Bioscience). Release of IL-23, IFN-γ, and IL-17 was instead assayed by specific ELISA kits (R&D Systems) or by human Th1/Th2 Cytokine kit (Cytometric Bead Array, CBA, BD Bioscience).
Cruciani M., Sandini S., Etna M.P., Giacomini E., Camilli R., Severa M., Rizzo F., Bagnoli F., Hiscott J, & Coccia E.M. (2019). Differential Responses of Human Dendritic Cells to Live or Inactivated Staphylococcus aureus: Impact on Cytokine Production and T Helper Expansion. Frontiers in Immunology, 10, 2622.
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2

Cytokine Profiling of Intestinal DCs

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To analyze the cytokine secretion profile of the intestinal DCs, CD11c+ cells were isolated by magnetic separation from intestinal single cell suspensions and stimulated in vitro with 1 μg/mL LPS for 20 hrs. IL-1β, IL-12, and IL-10 in the cell culture supernatants were quantitated with a BD Cytometric Bead Array (CBA from BD Biosciences, CA, USA) and FACS analysis following the manufacturer's protocol. The data were analyzed with FCAPArray software v1.0.1 (Soft Flow, St. Louis Park, MN, USA).
Dolpady J., Sorini C., Di Pietro C., Cosorich I., Ferrarese R., Saita D., Clementi M., Canducci F, & Falcone M. (2015). Oral Probiotic VSL#3 Prevents Autoimmune Diabetes by Modulating Microbiota and Promoting Indoleamine 2,3-Dioxygenase-Enriched Tolerogenic Intestinal Environment. Journal of Diabetes Research, 2016, 7569431.
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3

Cytokine Profile as a Diagnostic Panel for Cervical Dysplasia

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One hundred and seventeen patients with HR-HPV infection were divided according to the histopathological diagnosis as no cervical squamous intraepithelial lesion (NSIL; n = 43), low-grade squamous intraepithelial lesion (LSIL; n = 35), and HSIL (n = 39). There were no significant differences among groups with respect to age, HPV subtype, or pregnancy status. Seventy-four patients in the NSIL and LSIL groups were followed up for 3 years. The levels of IL-6, IL-2, IL-4, IL-17A, IL-10, tumor necrosis factor (TNF), and IFN-γ in vaginal lavage fluid were measured using a BD Cytometric Bead Array (CBA; BD Biosciences, San Jose, CA, USA) according to the manufacturer’s instructions. Statistically significant cytokines were converted to an algorithm as cytokine score, which was analyzed to be used as diagnostic panels by a logistic regression model. The follow-up was divided into two stages. The first stage was for 6 months; case data were collected and analyzed for the threshold of the cytokine score as a basis for grouping for the second stage (2.5 years) of follow-up. By comparing the results with TCT and HPV genotyping, a cohort study was conducted (Fig. 1).

Flowchart of the study design

Li B., Zhang L., Zhao J., Tan G., Zhang W., Zhang N., Tian J, & Qu P. (2019). The value of cytokine levels in triage and risk prediction for women with persistent high-risk human papilloma virus infection of the cervix. Infectious Agents and Cancer, 14, 16.
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4

Measuring Inflammatory Cytokines in Mice

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The proinflammatory cytokines interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor α (TNFα) were measured in plasma obtained from the mice at sacrifice. Bead-based immunoassays BD Cytometric Bead Array (CBA), from BD Biosciences, San Jose, CA, USA, were used according to the manufacturer's instructions. Data were collected on a BD LSRII flow cytometer (BD Biosciences) and analysed by use of FCAP Array software 3.0 (BD Biosciences). All samples were diluted 1 : 2 in Assay Diluent included in the CBA kit. The limits of detection were 1.9, 1.4 and 2.8 pg/mL, for IL-1β, IL-6 and TNFα, respectively.
Ngo H.T., Hetland R.B., Nygaard U.C, & Steffensen I.L. (2015). Genetic and Diet-Induced Obesity Increased Intestinal Tumorigenesis in the Double Mutant Mouse Model Multiple Intestinal Neoplasia X Obese via Disturbed Glucose Regulation and Inflammation. Journal of Obesity, 2015, 343479.
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5

Cytokine Quantification in PBMC and mo-DCs

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The concentration of IL-1β, IL-6, IL-8, IL-10, IL-12, and TNF in the supernatant of human PBMCs and human mo-DCs was determined using the BD™ cytometric bead array (CBA) (human inflammatory cytokine kit – 551811, BD Biosciences), according to manufacturer’s instructions.
Ferreira T.P., Mariano L.L., Ghilosso-Bortolini R., de Arantes A.C., Fernandes A.J., Berni M., Cecchinato V., Uguccioni M., Maj R., Barberis A., Silva P.M, & Martins M.A. (2016). Potential of PEGylated Toll-Like Receptor 7 Ligands for Controlling Inflammation and Functional Changes in Mouse Models of Asthma and Silicosis. Frontiers in Immunology, 7, 95.
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6

Cytokine Profiling of Nucleofected Monocytes

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Proinflammatory cytokines were measured in the supernatants of nucleofected and non-nucleofected monocytes using the BD Cytometric Bead Array (CBA) as per the manufacturer's instructions.
Bharaj P., Chahar H.S., Alozie O.K., Rodarte L., Bansal A., Goepfert P.A., Dwivedi A., Manjunath N, & Shankar P. (2014). Characterization of Programmed Death-1 Homologue-1 (PD-1H) Expression and Function in Normal and HIV Infected Individuals. PLoS ONE, 9(10), e109103.
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7

Cytokine and Transcript Quantification

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For measurements of IFNγ, TNF and IL-2 in comparison with RNA measurements over a 96 h time course, cytokine proteins were quantified using a BD cytometric bead array (CBA) from BD Biosciences and analysed by flow cytometry according to the manufacturer’s protocol. RNA was quantified using QuantiTect primer assays from Qiagen (QT01038821 and QT00104006 for mouse Ifng and Tnf, respectively) according to the manufacturer’s instructions. Relative transcript abundances were normalised to B2m.
Matheson L.S., Petkau G., Sáenz-Narciso B., D’Angeli V., McHugh J., Newman R., Munford H., West J., Chakraborty K., Roberts J., Łukasiak S., Díaz-Muñoz M.D., Bell S.E., Dimeloe S, & Turner M. (2022). Multiomics analysis couples mRNA turnover and translational control of glutamine metabolism to the differentiation of the activated CD4+ T cell. Scientific Reports, 12, 19657.
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8

Quantifying Cytokines and Chemokines in Plasma

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The cytometric bead array (CBA) test was employed to quantify cytokine and chemokines in plasma samples stored at − 80 °C. Beads, standards, reagents, and plasma samples were prepared according to the manufacturer's guidelines. Results were obtained using the BD Accuri flow cytometer and commercially available kits (BDTM Cytometric Bead Array) and commercial kits from BD™ Cytometric Bead Array (CBA) for “Human Chemokine” and “Human Inflammatory Cytokines” were used (BD Biosciences, USA). Standard dilution methods were applied for cytokine standards. Calibration of the flow cytometer with cytometer setup beads preceded the assay. Data analysis was performed in FCAP Array v2.0 software (SoftFlow, Pecs, Hungary), presenting results in pg/mL.
Santamarina A.B., de Freitas J.A., Franco L.A., Nehmi-Filho V., Fonseca J.V., Martins R.C., Turri J.A., da Silva B.F., Fugi B.E., da Fonseca S.S., Gusmão A.F., Olivieri E.H., de Souza E., Costa S., Sabino E.C., Otoch J.P, & Pessoa A.F. (2024). Nutraceutical blends predict enhanced health via microbiota reshaping improving cytokines and life quality: a Brazilian double-blind randomized trial. Scientific Reports, 14, 11127.
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9

CD44v6 CAR-T Cell Cytokine Profiling

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An E:T ratio of 1:1 was used when co‐culturing CD44v6 CAR‐T (or NT) cells with target cells in complete RPMI 1640 media for 24 h, after which the cytokines of supernatants were measured using the BD cytometric bead array (CBA).
Tang L., Huang H., Tang Y., Li Q., Wang J., Li D., Zhong Z., Zou P., You Y., Cao Y., Kong Y., Guo A., Zhou S., Li H., Meng F., Xiao Y, & Zhu X. (2022). CD44v6 chimeric antigen receptor T cell specificity towards AML with FLT3 or DNMT3A mutations. Clinical and Translational Medicine, 12(9), e1043.
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10

Modulating dendritic cell responses

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DCs were cultured in 1.5 × 105 cells/well in 200 μl, and where indicated pre-treated for 24h with CpG-A (5 μg/mL; InvivoGen), IFN-α (100U/ml, PBL Assay Science), Imiquimod (6 μM, Calbiochem), Poly I:C (25 μg/ml, Sigma), etomoxir (200 μM, Tocris Biochemicals), 5-(tetradecyloxy)-2-furoic acid (TOFA; 20 μM, Sigma Aldrich), UK-5099 (50 μM, Sigma Aldrich), MAR1-5A3 (5 μg/ml, Leinco Technologies) or GW6471 (3 μM; Tocris Biochemicals). pDCs were incubated overnight with 100 μM Gemfibrozil or 100 μM Muraglitazar (Santa Cruz). IFNα was detected using a mouse IFNα elisa kit (PBL assay science). TNF-α and IL-6 were measured using BD™ Cytometric Bead Array (CBA).
Wu D., Sanin D.E., Everts B., Chen Q., Qiu J., Buck M.D., Patterson A., Smith A.M., Chang C.H., Liu Z., Artyomov M.N., Pearce E.L., Cella M, & Pearce E.J. (2016). Type 1 interferons induce changes in core metabolism that are critical for immune function. Immunity, 44(6), 1325-1336.
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