THP-1 human monocyte-like cells (obtained from ATCC) were cultivated in RPMI with 10% FBS and 50 µM 2-mercaptoethanol. THP-1 cell lines inducibly expressing HA-tagged EGFP, ICEBERG, and INCA were generated using lentivirus produced with derivatives of pInducer2014 and selected in the presence of 500 µg/ml G418. Monoclonal cell lines were obtained by limited dilution in THP-1 medium with 20% FBS. For testing mycoplasma contamination, wild-type and derived THP-1 cells were stained with Hoechst 33342 and analyzed at high magnification. No punctate DNA staining was observed in the cytosol. Alexa Fluor 488 conjugated anti-HA mouse IgG monoclonal antibody (clone 16B12) from Life Technologies (Cat#A21287) was used to confirm HA-ICEBERG and HA-INCA expression in modified THP-1 cells.
Alexa fluor 488 conjugated anti ha mouse igg monoclonal antibody clone 16b12
Manufactured by Thermo Fisher Scientific
The Alexa Fluor 488 conjugated anti-HA mouse IgG monoclonal antibody (clone 16B12) is a fluorescently labeled antibody that specifically binds to the hemagglutinin (HA) epitope tag. The Alexa Fluor 488 dye provides a green fluorescent signal that can be detected using appropriate fluorescence detection methods.
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2 protocols using alexa fluor 488 conjugated anti ha mouse igg monoclonal antibody clone 16b12
1
Generation and Validation of THP-1 Cell Lines
2
Generation and Validation of THP-1 Cell Lines
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