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Iκbα sc 371

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IκBα (sc-371) is a protein-specific antibody produced by Santa Cruz Biotechnology. It is designed to detect IκBα (Inhibitor of NF-κB alpha) in various sample types.

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Lab products found in correlation

Pdlim2, by Santa Cruz Biotechnology (1 mentions) Ciap1, by R&D Systems (1 mentions) Gapdh mab374, by Merck Group (1 mentions) Anti il 6 ab6672, by Abcam (1 mentions) Nik sc 7211, by Santa Cruz Biotechnology (1 mentions) Anti ciap2, by Abcam (1 mentions) Parp 9532, by Cell Signaling Technology (1 mentions) Nf κb2 p100 p52, by Cell Signaling Technology (1 mentions) α tubulin sc 8035, by Santa Cruz Biotechnology (1 mentions) Flag tag m2, by Merck Group (1 mentions) Recombinant human tnf α, by Thermo Fisher Scientific (1 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions) β actin sc 47778, by Santa Cruz Biotechnology (1 mentions) Lipopolysaccharide lps, by Merck Group (1 mentions) Phospho iκbα ser32, by Cell Signaling Technology (1 mentions) Il 1β af 401 na, by R&D Systems (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Caspase 1, by Adipogen (1 mentions) Ag 20b 0042 c100, by Adipogen (1 mentions) Nlrp3 ag 20b 0014 c100, by Adipogen (1 mentions)

7 protocols using iκbα sc 371

1

Immunohistochemical Staining of Lymphomas

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Immunohistochemical stainings of 20 primary cHL, 10 follicular lymphoma, 15 diffuse large B-cell lymphoma and 10 Burkitt lymphoma cases were performed as previously described.33 (link) Primary antibodies (PDLIM2, PA5-31484, Thermo Scientific, Darmstadt, Germany; PDLIM2, ab68220, Abcam; IκBα, sc-371, Santa Cruz) were applied in a dilution of 1:100 (PDLIM2 antibodies) or 1:500 (IκBα) overnight.
Wurster K.D., Hummel F., Richter J., Giefing M., Hartmann S., Hansmann M.L., Kreher S., Köchert K., Krappmann D., Klapper W., Hummel M., Wenzel S.S., Lenz G., Janz M., Dörken B., Siebert R, & Mathas S. (2016). Inactivation of the putative ubiquitin-E3 ligase PDLIM2 in classical Hodgkin and anaplastic large cell lymphoma. Leukemia, 31(3), 602-613.
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2

Immunoblot Analysis of Cell Signaling

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Whole-cell lysates were obtained and analyzed by immunoblot as previously described.39 (link) The following primary antibodies were used: cIAP-1 (1:2000) from R&D Systems; anti-cIAP-2 (1:1000) and anti-IL-6 (ab6672; 1:1000) from Abcam; actin (sc-1615; 1:1000), IκBα (sc-371; 1:1000), NIK (sc-7211; 1:1000) from Santa Cruz Biotechnology (Santa Cruz, CA, USA); RIP1 (BD 610458; 1:1000) from BD Biosciences (San Jose, CA, USA); NF-κB2 p100/p52 (#4882; 1:1000) and PARP (#9532; 1:1000) from Cell Signaling Technology (Beverly, MA, USA); GAPDH (MAB374; 1:5000) from EMD Millipore (Temecula, CA, USA).
Guicciardi M.E., Krishnan A., Bronk S.F., Hirsova P., Griffith T.S, & Gores G.J. (2017). Biliary tract instillation of a SMAC mimetic induces TRAIL-dependent acute sclerosing cholangitis-like injury in mice. Cell Death & Disease, 8(1), e2535-.
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3

Platelet Whole Cell Lysate Immunoblotting

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Platelet whole cell lysates were prepared in ELB buffer and immunoblot analysis was performed as described [38 ]. The following primary antibodies were used: IKKα (sc-7218), IKKβ (sc-8014), IκBα (sc-371), and α-Tubulin (sc-8035; 1:1000; Santa Cruz Biotechnologies Inc., Santa Cruz, CA, USA).
Kiebala M., Singh M.V., Piepenbrink M.S., Qiu X., Kobie J.J, & Maggirwar S.B. (2015). Platelet Activation in Human Immunodeficiency Virus Type-1 Patients Is Not Altered with Cocaine Abuse. PLoS ONE, 10(6), e0130061.
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4

Plasmid and siRNA Transfection Protocol

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Transfection of plasmid DNA and siRNAs were performed by GeneJuice (Novagen) and Lipofectamine RNAiMAX (Life technologies), respectively, according to the manufacturer's instructions. Stealth RNAs for negative controls and human NPM1 were described previously (32 (link)). Mouse NPM1 (s124573) and human NF-κB p65 (HSS109159) were obtained from Life technologies. Antibodies used were NPM1 (Invitrogen), Flag tag (M2, Sigma Aldrich), p65 (ab7970, Abcam), I-κBα (sc-371, Santa Cruz) and β-actin (sc-47778, Santa Cruz). Recombinant human TNF-α (PEPROTECH) and Lipopolysaccharide (LPS) (Sigma) were commercially available.
Lin J., Kato M., Nagata K, & Okuwaki M. (2016). Efficient DNA binding of NF-κB requires the chaperone-like function of NPM1. Nucleic Acids Research, 45(7), 3707-3723.
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5

NLRP3 Inflammasome Signaling Pathway

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Antibodies used for western blot were IL-1β (AF-401-NA, R&D System), Caspase-1 (AG-20B-0042-C100, AdipoGen Life Science), NLRP3 (AG-20B-0014-C100, AdipoGen Life Science), ASC (AG-25B-0006-C100, AdipoGen Life Science), phospho-IκBα (Ser32) (#2859, Cell Signaling), IκBα (sc-371, Santa Cruz) and β-actin (#3700, Cell Signaling).
Lee A.H., Shin H.Y., Park J.H., Koo S.Y., Kim S.M, & Yang S.H. (2021). Fucoxanthin from microalgae Phaeodactylum tricornutum inhibits pro-inflammatory cytokines by regulating both NF-κB and NLRP3 inflammasome activation. Scientific Reports, 11, 543.
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6

Characterization of NF-κB signaling components

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Purified polyclonal antibodies against human p65 NFκB (sc-372), phosphorylated p65 NFκB at S536 (sc-33020), p50 NFκB (sc-7178), and IκBα (sc-371) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Purified polyclonal antibody against actin was from Sigma (St. Louis, MO, USA). Horseradish peroxidase (HRP)-conjugated anti-rabbit, anti-mouse and anti-goat secondary antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA). Bortezomib was obtained from Selleck Chemicals (Houston, TX, USA). The IKK inhibitor Bay 117085 was purchased from Cayman Chemicals (Ann Arbor, MI, USA). All other reagents were molecular biology grade and were from Sigma (St. Louis, MO, USA).
Singha B., Gatla H.R., Phyo S., Patel A., Chen Z.S, & Vancurova I. (2015). IKK inhibition increases bortezomib effectiveness in ovarian cancer. Oncotarget, 6(28), 26347-26358.
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7

Western Blotting for Protein Analysis

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Western blotting was carried out as described previously (39 (link)). Following cell lysis, proteins were size-fractionated in SDS-PAGE gels. Following electrophoresis, the proteins were transferred to a nitrocellulose membrane followed by blocking and probing with primary antibodies against TLR2 (12276, Cell Signaling and ab108998, Abcam), GFP (2555, Cell Signaling), IκBα (sc-371, SantaCruz), Ser32/Ser36 phosphorylated-IκBα (9246, Cell Signaling), p65 (sc-8008, SantaCruz), Ser536 phosphorylated-p65 (3036, Cell Signaling), GR (PA1-511A, Thermo Fisher Scientific), MAPKAPK2 (3042, Cell Signaling), Thr334 phosphorylated-MAPKAPK2 (3041, Cell Signaling), total p38 (9212, Cell Signaling), Thr180/Tyr182 phosphorylated-p38 (9211, Cell Signaling), p38α (9218, Cell Signaling), p38β (2339, Cell Signaling), or GAPDH (MCA4739, Bio-Rad), overnight at 4 ºC. Membranes were washed in Tris Buffer Saline-Tween 20 followed by incubation with 1:5000 or 1:10,000 dilution of the respective rabbit or mouse horseradish peroxidase-conjugated secondary immunoglobulin (Jackson ImmunoResearch) at room temperature. Membranes were washed 4 × 5 min prior to detection of immune complexes by enhanced chemiluminescence (Bio-Rad). Images were acquired using a ChemiDoc Touch imaging system (Bio-Rad), and densitometric analysis was performed using ImageLab software (Bio-Rad).
Bansal A., Mostafa M.M., Kooi C., Sasse S.K., Michi A.N., Shah S.V., Leigh R., Gerber A.N, & Newton R. (2022). Interplay between nuclear factor-κB, p38 MAPK, and glucocorticoid receptor signaling synergistically induces functional TLR2 in lung epithelial cells. The Journal of Biological Chemistry, 298(4), 101747.
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