Gallic acid monohydrate

All chemicals used in the present study were of analytical HPLC grade and were purchased from Hi-media, India and Sigma-Aldrich, USA. ABTS (2,2- Azinobis-3-ethylbenzothiazoline-6-sulfonic acid), 2,2-diphenyl-1-picrylhydrazyl (DPPH), Dimethyl Sulfoxide, Sodium acetate trihydrate ACS, Ferric chloride hexahydrate A.R., Ferrous sulfate heptahydrate A.R., Folin ciocalteu's reagent L.R., Gallic acid monohydrate, L-Ascorbic acid A.R., Acetic acid glacial A.R., Sodium carbonate ACS, Potassium persulphate A.R. were purchased from Hi-media (India). 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (trolox), Aluminum chloride AR, and Quercetin ≥ 95% (HPLC) solid were purchased from Sigma-Aldrich (USA). All chemicals and reagents were used without any further purification.

2,2- Azinobis-3-ethylbenzothiazoline-6-sulphonic acid disodium salt (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), Dimethyl Sulphoxide, Sodium acetate trihydrate ACS, Ferric chloride hexahydrate A.R., Ferrous sulphate heptahydrate A.R., Folin ciocalteu’s reagent L.R., Gallic acid monohydrate, L-Ascorbic acid A.R., Acetic acid glacial A.R., Sodium carbonate ACS, Potassium persulphate A.R., were purchased from Hi-media, Mumbai, India. 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (trolox), Aluminium chloride AR, and Quercetin ≥95% (HPLC) solid were purchased from Sigma-Aldrich, USA. Acetonitrile, methanol (LC-MS grade) and formic acid (analytical grade) were purchased from Fluka, Sigma-Aldrich (St. Louis, MO, USA). Ultra pure water was obtained from a Direct-Q 8 UV water purification system (EMD Millipore Corporation, Billerica, MA, USA). All other reagents including solvents were of analytical grade and were procured from Hi-Media, Mumbai, India.