The procedure for middle cerebral artery occlusion (MCAO) was performed as described previously (Sun et al., 2018). The rats were sedated and their body temperature was maintained at 37°C using a thermostatic pad (Globalebio, Beijing, China). The left common carotid, internal carotid, and external carotid arteries were all exposed through a midline skin incision. To block the origin of the middle cerebral artery, a silicone-coated nylon monofilament (diameter 0.32 ± 0.02 mm, Cinontech, Beijing, China) was gently introduced through the midline skin incision into the internal carotid artery lumen. Reperfusion was initiated 90 minutes after MCAO by removing the suture. After 30 minutes of ischemia, left cervical VNS was applied using a Grass model S48 stimulator (Grass Technologies, Warwick, RI, USA), which administered a 20-Hz, 0.5-ms (0.5 mA) square pulse train lasting 30 seconds, every 4 minutes for 1 hour, in accordance with the method used in a previous study (Li et al., 2020b). The control rats’ cervical skin was opened and wrapped with moist gauze, but these rats were not subjected to MCAO or stimulation.
Grass model s48 stimulator
Manufactured by Natus
Sourced in United States
The Grass model S48 stimulator is a laboratory instrument designed to provide electrical stimulation for various research applications. It generates programmable electrical pulses that can be used to stimulate biological samples or systems. The core function of the S48 stimulator is to deliver controlled electrical signals to enable the study of physiological responses or to elicit specific reactions in the subject under investigation.
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Lab products found in correlation
2 protocols using grass model s48 stimulator
1
Rat Middle Cerebral Artery Occlusion Model
2
Vagus Nerve Stimulation in tMCAO Mice
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VNS was performed firstly at 30 mins, 1 day, and 2 days after the establishment of tMCAO. Invasive VNS was performed as previously described (28 (link)). Briefly, the mice were anesthetized intraperitoneally with 3.5% chloral hydrate (0.1ml/10g). Bipolar electrodes were wrapped delicately around the right cervical VN and sutured to the sternocleidomastoid muscle. VNS (0.5 mA, 5 Hz) was administered through a Grass Model S48 stimulator (Grass Technologies, USA) for 30 seconds every five minutes for one hour.
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