Wistar male rats were purchased from Janvier Laboratories and housed in a temperature-controlled room maintained on a 12 h day/night cycle with food and water provided ad libitum. Animal experiments were performed in compliance with and with the approval of the local ethics committee (agreement 2015101320441671, 2016–2021), standards for the care and use of laboratory animals, and the French and European Community guidelines as well as the Massachusetts General Hospital’s Institutional Animal Care and Use Committee.
Male wistar rats
Manufactured by Janvier Labs
Sourced in France
Male Wistar rats are laboratory animals commonly used in research and testing. They are a commonly used rodent strain known for their characteristics such as being docile, easy to handle, and having a good breeding performance.
Automatically generated - may contain errors
Lab products found in correlation
Myograph, by Danish Myo Technology (2 mentions)
Methoxamine, by Merck Group (2 mentions)
Linopirdine, by Merck Group (2 mentions)
Epigallocatechin gallate (egcg), by Merck Group (2 mentions)
Powerlab, by ADInstruments (2 mentions)
Chart software, by ADInstruments (2 mentions)
C57bl 6 male mice, by Janvier Labs (1 mentions)
Cypd knock out mice ppif mice, by Jackson ImmunoResearch (1 mentions)
C57bl 6j male mice, by Janvier Labs (1 mentions)
Male brown norway rats, by Charles River Laboratories (1 mentions)
Balb cbyj mice, by Charles River Laboratories (1 mentions)
Female c57bl 6jrj mice, by Janvier Labs (1 mentions)
Rompun vet, by Bayer (1 mentions)
Vivid 9, by GE Healthcare (1 mentions)
Female lewis rats, by Charles River Laboratories (1 mentions)
Collagenase type 4, by Worthington (1 mentions)
C57bl 6j, by Charles River Laboratories (1 mentions)
Of1 mice, by Charles River Laboratories (1 mentions)
Male swiss mice, by Janvier Labs (1 mentions)
56 protocols using male wistar rats
1
Wistar Rat Housing and Care
2
Wistar Rat Housing and Experimental Procedures
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Wistar male rats (5‐week‐old, 200–220 g, Janvier Labs, Le Genest‐Saint‐Isle, France) were housed at the animal care facility of the HP2 Laboratory (approval no. A38 51610006) under a 12:12 hours light–dark cycle at 20°C to 22°C and allowed free access to standard food and water. The experimental procedures were conducted in accordance with the European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes (Council of Europe, European Treaties ETS 123, Strasbourg, 18 March 1986) and with the Guide for Care and Use of Laboratory Animals (NIH Publication No. 85‐23, revised 1996) and were approved by an Institutional Animal Care and Use Committee (agreement number 2015032010109170 (APAFIS#695)).
3
Gut Inflammation in Aged Rats with Probiotic
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Twenty months (n = 45; old) Wistar male rats (Janvier, Le Genest Saint Isle, France) were housed individually and kept in a controlled environment (temperature maintained at 22°C; 12:12 light: dark cycle). After an adaptation period during fed the regular chow, animals were divided into three groups [n = 16 per group: PF (control pair fed), DSS (DSS treated), PB1 (DSS + probiotic CNRZ160)] and homogenized for body weight and lean mass as described above for adult rats. In the same manner as in the study carried out with adult animals, low grade gut inflammation was induced by adding 4% (w/v) of the same batch of DSS to the drinking water for four weeks (DSS and PB1 group). PB1 group received daily 5 × 109 CFU of the bacterial strains S. thermophilus CNRZ160. Control of food and water intake, weight and body composition were carried out as previously described in adult animals.
All samplings and injections (13C valine for protein synthesis assessment) were carried out as previously described in adult animals. However, additional measurements were performed in old animals. In particular, thorough analyses have been done on plasma and tissue samples for a better evaluation of inflammatory and metabolic status at local and systemic.
All samplings and injections (13C valine for protein synthesis assessment) were carried out as previously described in adult animals. However, additional measurements were performed in old animals. In particular, thorough analyses have been done on plasma and tissue samples for a better evaluation of inflammatory and metabolic status at local and systemic.
4
Wistar Rat Housing Conditions
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Subjects 237 young adult Wistar male rats, weighing 200-225 g at their arrival in the laboratory, were purchased from Janvier Labs (France) and randomly housed in groups of two in home cages placed on a ventilated cage rack (Tecniplast, France). We maintained the rats in a light-(12h reverse light-dark cycle, light off at 8 am) and temperature-controlled vivarium (22°C). We
5
Wistar Rat Acclimatization Protocol
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Male Wistar rats (n = 71) from Janvier Labs (Saint‐Berthevin, France) weighing around 250–350 g on the day of experiments were used for this study and housed at Syncrosome Laboratories (Marseille, France). The acclimatization of the animals lasted at least 5 days. They had free access to food and drinking water ad libitum. The experimental procedures were carried out in accordance to European guidelines for the care and use of laboratory animals (Directive 2010/63/UE) and were approved by the French Ethical Committee C2EA‐/71, of which Syncrosome is a member. Every effort was done to maintain animal's health, safety and welfare, minimize suffering and reduce the number of animals used in the experiments. Furthermore, Syncrosome laboratories and its animal housing facilities are accredited by French Authorities and audited every year by French Department of Veterinary Service.
6
Animal Study Protocols for Rats and Mice
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Male Wistar rats (280–300 g) and male C57BL6 mice (20–30 g) were purchased from Janvier Labs (Le Genest-Saint-Isle, France). They were housed in climate-controlled conditions and had unrestricted access to standard rat chow and drinking water. Animal experiments were performed in accordance with the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health (NIH Pub. No.85–23, Revised 1996), the Council of the European Communities (86/609/EEC; November 24th 1986), the French National Legislation (Ethical Approval No.76–114/ 01181.01) and also by the Biomedical Ethics Committee of the Pasteur Institute in Tunis (Ethical Approval No. 2015/08/I/LR11IPT08/V0; 9 November 2015).
7
Mesenteric Artery Tension Measurements
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Male wistar rats, 12 weeks old (Janvier Labs, France) were euthanized by cervical dislocation and used in accordance with Directive 2010/63EU on the protection of animals used for scientific purposes, approved by the national ethics committee, Denmark. Rats were group-housed with regular 12-hour light/dark cycles, in clear plastic containers with ad libitum access to food and water and underwent at least one week of habituation. The intestines were removed, and third-order mesenteric arteries were dissected in ice-cold physiological saline solution containing (in mM): 121 NaCl, 2.8 KCl, 1.6 CaCl2, 25 NaHCO3, 1.2 KH2HPO4, 1.2 MgSO4, 0.03 EDTA, and 5.5 glucose. Segments, 2 mm in length, of mesenteric artery were mounted on 40 μm stainless steel wires in a myograph (Danish Myo Technology, Aarhus, Denmark) for isometric tension recordings. The chambers of the myograph contained PSS maintained at 37°C and aerated with 95% O2/5% CO2. Changes in tension were recorded by PowerLab and Chart software (ADInstruments, Oxford, United Kingdom). The arteries were equilibrated for 30 minutes and normalized to passive force. Artery segments were precontracted with 10 μM methoxamine (Sigma; Copenhagen, Denmark) in the absence or presence of linopirdine (10 μM) (Sigma; Copenhagen, Denmark), before application of ECG, EGCG or EC (Sigma; Copenhagen, Denmark).
8
Mesenteric Artery Tension Measurements
9
Dietary Exposure and Colitis Induction in Rats
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Male Wistar rats (140-170g body weight, 4 weeks old, Janvier, France) were maintained in the Toxalim animal facil2ity (INRAE, UMR 1331, Toulouse), with free access to food and water throughout the study. The experiments were carried out in accordance with the European Guidelines for the care and use of animals for Scientific Purposes and validated by Toxcomethique Ethics Committee and the French Ministry of National Education Higher Education and Research (APAFIS#4832-2016040716495113). After five days of acclimatization, the animals received the control, DON or DOM-1 contaminated diet (8 mg.kg - 1 ) for four weeks. In the last week, colitis was induced in some animals by adding DSS (0, 2, 3 or 5%) to the drinking water (Supplementary Material Figure 1) for seven days and the rats were then euthanized by cervical dislocation and exsanguination. A persistently hunched posture and labored respiration, a weight loss of more than 20% were considered as end-points at which the animals were euthanized.
10
Animal Models for Mitochondrial Studies
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Male C57BL/6J mice (8 to 10 week-old) and male Wistar rats (250–300 g) were purchased from Janvier (Le Genest-St-Isle, France). CypD knock-out mice (Ppif−/− mice) were obtained from Jackson Laboratories (Bar Harbor, Maine, USA). Animals were housed in an air-conditioned room with a 12 h light–dark cycle and received standard rodent chow and drinking water ad libitum. All animal procedures used in this study were in accordance with the Directives of the European Parliament (2010/63/EU-848 EEC) and were approved by the Animal Ethics Committee Afssa/ENVA/Universite Paris Est Creteil (approval number 09/12/14-02).
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