Total RNA from tracheal grafts was isolated using Trizol (Thermo Fisher Scientific). After quantification of nucleic acids by spectrophotometry (Nanodrop, Thermo Fisher Scientific), 2 μg of the total RNA was reverse transcribed using PrimeScript RT Reagent Kit (Takara). cDNA products were amplified on a ABI 7500 Fast Real-Time PCR System in 20 μL of reaction mixture containing the SYBR GreenER qPCR Super-Mix Universal (Takara) and 10 μM of forward and reverse primers: IDO forward: GAGTAGACAGCAATGGCA; IDO reverse: AGTGGATGTGGTAGAGCA; β-actin forward: CTACAATGAGCTGCGTGTG; β-actin reverse: GCGTGAGGGAGAGCATAG (95 °C for 15 s, 55 °C for 1 min, and 65 °C for 1 min, 35 cycles). Results are expressed relative to the reference gene β-actin.
Sybr greener qpcr super mix universal
Manufactured by Takara Bio
The SYBR GreenER qPCR Super-Mix Universal is a ready-to-use reagent for quantitative real-time PCR (qPCR) analysis. It contains SYBR Green I dye, which binds to double-stranded DNA, and all the necessary components for qPCR amplification and detection.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using sybr greener qpcr super mix universal
1
Quantification of Tracheal Graft IDO Expression
2
RNA Extraction and qRT-PCR Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA was isolated from tissue or cell samples with TRIzol reagent (ThermoFisher, Carlsbad, CA, USA). Then, PrimeScript RT reagent Kit (Takara, Beijing, China) was used for reverse transcription. qRT-PCR was conducted using SYBR GreenER qPCR SuperMix Universal (Takara).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!