To study induction of apoptosis over a period of time, A549 cells were treated with 1.25 µM, 2.5 µM, and 5 µM concentrations of DJ4 for 16-, 24-, and 48 h. MDA-MB-231 cells were treated with 2.5 µM and 5 µM concentrations of DJ4 for 48 h. The cells were trypsinized with 0.05% trypsin. Floating cells were also collected for analysis. The samples were incubated with MuseTM Annexin V reagent and analyzed by laser-based fluorescent detection using MuseTM (Millipore Inc.) cell analyzer per manufacturer’s instructions.
Muse
Manufactured by Merck Group
Sourced in United States
The Muse™ is a compact, portable, and easy-to-use lab equipment designed for various scientific applications. It serves as a versatile tool for researchers and professionals in the field of life sciences. The core function of the Muse™ is to provide accurate and reliable data collection and analysis capabilities for a wide range of experimental procedures.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using muse
1
Apoptosis Induction Kinetics in A549 and MDA-MB-231 Cells
2
Mitochondrial Depolarization and Apoptosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells undergoing mitochondrial inner membrane depolarization were analyzed to understand the underlying apoptosis pathway of cellular death. MDA-MB-231 cells were treated with indicated concentrations of DJ4 and incubated for 48 h. The cells were trypsinized with 0.05% trypsin. Floating cells were collected for analysis. The samples were incubated with MuseTM Mito Reagents and analyzed by laser-based fluorescent detection using MuseTM (Millipore Inc., Burlington, MA, USA) per manufacturer’s instructions.
3
DJ4 Cytotoxicity in MDA-MB-231 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MDA-MB-231 cells were treated for 24 h with 1 µM, 2.5 µM, 5 µM, and 10 µM concentrations of DJ4. Cells were trypsinized with 0.05% trypsin. Floating cells were collected and included in the analysis. Cell number and cell viability were analyzed using MuseTM Cell Viability reagent and MuseTM (Millipore, Inc. Burlington, MA, USA) cell analyzer as per the manufacturer’s instructions.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!