The largest database of trusted experimental protocols

5 protocols using calcium acetate

1

Lipid-Based Nanoparticle Delivery of ATRA and Anti-PD-1 Antibody

Check if the same lab product or an alternative is used in the 5 most similar protocols
Hydrogenated soybean phosphatidylcholine (HSPC), 1, 2-distearoyl-sn-glycero -3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)-2000] (DSPE-PEG2000) were purchased from Lipoid (Germany). Cholesterol and fluorescein labeled PEG-DSPE were from Avanti Polar Lipids (AL, USA). All-trans retinoic acid (ATRA) was obtained from Shandong Liangfu Pharmaceutical Co., Ltd. Other reagents including ethanol, calcium acetate, sodium chloride, potassium chloride and sucrose were of analytical reagent grades and purchased from Sinopharm (Shanghai, China). Fetal bovine serum and hydroxypropyl-β-cyclodextrin (HPCD) were from Sigma-Aldrich (Steinheim, Germany). Acetonitrile and methanol (HPLC grade) were from Thermo Fisher Scientific (Waltham, USA). Therapeutic mouse anti-PD-1 were purchased from BIO X CELL™(USA), InVivoMAb Antibodies,anti-mouse PD-1(CD279,Clone: 29F.1A12).
+ Open protocol
+ Expand
2

Synthesis of Cobalt Ferrite Nanocomposites

Check if the same lab product or an alternative is used in the 5 most similar protocols
All the chemical reagents used in the preparation of materials are of analytical grade. Barium acetate, calcium acetate, tetrabutyl titanate, ethylene glycol, and glacial acetic acid were purchased from Sinopharm Group Chemical Reagent Co., Ltd. CoFe2O4 material with an average nanoparticles diameter of ∼40 nm was purchased from Beijing Dk Nano Technology Co., LTD. PVP was purchased from Zhangjiakou Kerma Fine Chemicals Co., Ltd.
+ Open protocol
+ Expand
3

Copper-Alginate Hydrogel Synthesis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sodium alginate (NaAlg) was purchased from the Guangfu Fine Chemical Research Institute (Tianjin, China). Pentahydrate copper sulfate (CuSO4·5H2O) was obtained from the Guangcheng Chemical Reagent Co., Ltd. (Tianjin, China). Both ammonia water (NH3·H2O) and sodium hydroxide (NaOH) were supplied by the fine chemical plant of the Laiyang Economic and Technological Development Zone (Yantai, China). Calcium acetate and ascorbic acid were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). All the chemicals were of analytical grade and not further purified, and all the solutions were prepared using deionized water.
+ Open protocol
+ Expand
4

Synthesis of Inorganic Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sodium hydroxide (NaOH), aluminum hydroxide (Al(OH)3), calcium chloride (CaCl2), calcium acetate ((CH3COO)2Ca), sodium carbonate (Na2CO3) and sucrose (C12H22O11) were obtained from Sinopharm Chemical Reagent Co., Ltd., China. All the reagents used were of analytical grade. The ordinary ground calcium carbonate powder was obtained from a factory in Hezhou city Guangxi Province in China. Deionized water was applied throughout this study.
+ Open protocol
+ Expand
5

Hydrothermal Synthesis of α-CSH and OCP

Check if the same lab product or an alternative is used in the 5 most similar protocols
α-CSH was prepared using the hydrothermal method. In brief, 20 mL of crystal modifier containing 2 wt% magnesium sulfate heptahydrate (Sinopharm Chemical Reagent Co., Ltd.) and 0.15 wt% sodium citrate (Sinopharm Chemical Reagent Co., Ltd.) was added to 6.7 g of calcium sulfate dihydrate (Sinopharm Chemical Reagent Co., Ltd.) at 0.15 MPa and 130°C for 5 hours.46 (link) The material was dried in the oven for 4 hours at 110°C, and a powder was generated by grinding and sifting.
OCP was prepared by the homogeneous precipitation method.47 The 0.04 M calcium acetate (Sinopharm Chemical Reagent Co., Ltd.) solution was trickled slowly into the 0.04 M sodium dihydrogen phosphate (Sinopharm Chemical Reagent Co., Ltd.) solution with a drop funnel, and the mixture was stirred at 68°C for 1 hour and reacted for 2 hours. The sediment was filtered, and the powder was washed with distilled water and dried.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!