Ns1643

Manufactured by Alomone

Sourced in Israel

NS1643 is a potassium channel activator. It has been used in experimental research settings.

Automatically generated - may contain errors

Lab products found in correlation

4 protocols using ns1643

Breast Cancer Cell Lines Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

MDA-MB-231, SKBR3, and MCF7 human BC cell lines were obtained from the American Type Culture Collection (ATCC) (Manassas, VA) and maintained 231 cells (ATCC) was maintained in Dulbecco’s modified Eagle’s medium (DMEM) (4.5 g/L glucose) supplemented with 10% fetal bovine serum, Penicillin (100 µg/ml)/streptomycin (100 µg/mL) antibiotics. Cells were incubated under humidified conditions with 5% CO₂ at 37 °C.
Antibodies against phospho-β-catenin^S552, phospho-β-catenin^S33/S37/T41, β-catenin, E-cadherin, N-cadherin, Vimentin, CD44, phospho-GSK3β^S9, GSK3β, phospho-AKT^T308, AKT, pan-cytokeratin and Actin were purchased from Cell Signaling Technologies, Inc (Boston, MA). An anti-HNA antibody was purchased from Abcam (Cambridge, UK). p16^INK4a was purchased from Abcam.
Cycloheximide (CHX) was purchased from Sigma-Aldrich (St. Louis, MO) and NS1643 was purchased from Alomone Labs (Jerusalem, Israel).

Breuer E.K., Fukushiro-Lopes D., Dalheim A., Burnette M., Zartman J., Kaja S., Wells C., Campo L., Curtis K.J., Romero-Moreno R., Littlepage L.E., Niebur G.L., Hoskins K., Nishimura M.I, & Gentile S. (2019). Potassium channel activity controls breast cancer metastasis by affecting β-catenin signaling. Cell Death & Disease, 10(3), 180.

+ Open protocol

+ Expand

Cell Culture and Reagent Procurement Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

HT29 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (4.5 g/L glucose) (Corning, Manassas, VA, USA) with 1% GlutaMax (Gibco, Waltham, MA, USA), FET cells in DMEM/F12 (50:50) media (Corning, Manassas, VA, USA), and SW480 cells in DMEM media, supplemented with 10% fetal bovine serum (FBS) (Gemini Bio, West Sacramento, CA, USA) and 1% penicillin (10,000 units/mL)/streptomycin (10,000 µg/mL) (Gibco, Waltham, MA, USA) at 37 °C and 5% CO₂. All antibodies were purchased from Cell Signaling Technologies, Inc (Boston, MA, USA). An anti-KCNH2 antibody was purchased from Santa Cruz (Dallas, Texas, USA) or Alomone Lab (Islrael). Recombinant human TGF-β1 and anti-hTGF-β RII FITC conjugated were purchased from R&D Systems (Minneapolis, MN, USA). E4031 and NS1643 were purchased from Alomone Labs (Jerusalem, Israel). Sanguinarine chloride was purchased from Tocris (Bristol, UK). Cyclosporine A was purchased from LC Laboratories (Woburn, MA, USA).

Eskandari N., Senyuk V., Moore J., Kalik Z., Luan Q., Papautsky I., Moshiri A., Bocchetta M., Salami S.A., Oryan S, & Gentile S. (2021). Molecular Activation of the Kv11.1 Channel Reprograms EMT in Colon Cancer by Inhibiting TGFβ Signaling via Activation of Calcineurin. Cancers, 13(23), 6025.

+ Open protocol

+ Expand

Investigating Cell Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols

Rabbit Anti AMPK, p-AMPK ^T172, Pro-Caspase 3, ULK, p-ULK ^S555, p21^WAF, cyclin E, D, & B, MEK, p-MEK, ERK 1/2, p-ERK ½ and pWEE antibodies were purchased from Cell Signaling Technologies, Inc. (Boston, MA, USA). p16INK4A was purchased from Abgent (San Diego, USA, CA). The Kv 11 agonist NS1643 was purchased form Alomone Labs (Jerusalem, Israel).

Perez-Neut M., Haar L., Rao V., Santha S., Lansu K., Rana B., Jones W.K, & Gentile S. (2016). Activation of hERG3 channel stimulates autophagy and promotes cellular senescence in melanoma. Oncotarget, 7(16), 21991-22004.

+ Open protocol

+ Expand

Cell Line Maintenance and Antibody Validation

Check if the same lab product or an alternative is used in the 5 most similar protocols

All cell lines were purchased from ATCC and maintained at 37°C and 5% CO₂ in Dulbecco’s modified Eagle’s medium (DMEM, 4.5 g/L glucose), supplemented with 10% fetal bovine serum, non-essential amino acids, 1% Penicillin (100 mg/ml) and streptomycin (100 mg/mL) antibiotics. Mouse anti-Ki-67, rabbit anti-phospho-ERK1/2, ERK1/2, p21^waf/cip, and HMGA2 antibodies were purchased from Cell Signaling Technologies, Inc (Boston, MA, USA). Mouse anti-p16^INK4A antibody was purchased from Sigma (St. Louis, MO, USA), Mouse anti-phospho H2AX antibody was purchased from EMD Millipore (Darmstadt, Germany), and rabbit anti-H2AX antibody was purchased from Abcam (Cambridge, MA, USA). Horse anti-mouse and goat anti-rabbit secondary antibodies were purchased from Cell Signaling Technologies, Inc. (Boston, MA, USA). NS1643 was purchased from Alomone Labs (Jerusalem, Israel), EGTA was purchased from Calbiochem (San Diego, CA, USA), TEMPOL was purchased from Sigma (St. Louis, MO, USA) and E-4031 was purchased from Abcam (Cambridge, MA, USA).

Fukushiro-Lopes D.F., Hegel A.D., Rao V., Wyatt D., Baker A., Breuer E.K., Osipo C., Zartman J.J., Burnette M., Kaja S., Kouzoukas D., Burris S., Jones W.K, & Gentile S. (2017). Preclinical study of a Kv11.1 potassium channel activator as antineoplastic approach for breast cancer. Oncotarget, 9(3), 3321-3337.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!