Gentamicin

Manufactured by Henry Schein

Gentamicin is a broad-spectrum antibiotic used in laboratory settings. It is effective against a variety of gram-negative and some gram-positive bacteria. Gentamicin functions by inhibiting bacterial protein synthesis, leading to cell death.

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Lab products found in correlation

Heparin, by Henry Schein (1 mentions)

5 protocols using gentamicin

Surgical Implantation of Jugular Catheters in Rats

Cited 3 times

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Two weeks after the non-contingent saline or METH injection, rats were anesthetized with an intraperitoneal injection of ketamine (50 mg/kg) and xylazine (5 mg/kg) for surgical insertion of polyurethane catheters (SAI Infusion Technologies, Lake Villa, IL) into the jugular vein as previously described [5 (link)]. The other end of the catheter was attached to an external back mount device that allowed for access to the catheter. These ports were closed with dust caps (Plastics One, Roanoke, VA). After surgery, rats were given subcutaneous injections of buprenorphine (0.1 mg/kg) to relieve pain. Rats were allowed one week to recover post-surgery. During recovery, and throughout the SA experiment, catheters were flushed every 48 h with 1 mL gentamicin (0.05 mg/ml, Henry Schein, Melville, NY) in sterile saline solution.
We selected to use the 3-week time-point to perform SA experiments after the non-contingent METH injection because of previous studies in which we had documented enhanced changes in gene expression in rats that were euthanized one month after a single injection of METH (10 mg/kg) [40 (link)]. Increased expression of stress-related genes was apparent after 2 weeks and remained elevated for one month [72 (link)].

Jayanthi S., Torres O.V., Ladenheim B, & Cadet J.L. (2019). A Single Prior Injection of Methamphetamine Enhances Methamphetamine Self-Administration (SA) and Blocks SA-Induced Changes in DNA Methylation and mRNA Expression of Potassium Channels in the Rat Nucleus Accumbens. Molecular Neurobiology, 57(3), 1459-1472.

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Post-Operative Catheter Maintenance

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On the two days following surgery, rats were administered Rimadyl (5 mg/kg) subcutaneously. Catheter patency was maintained by daily infusion of 0.2–0.4 ml of a 0.99% sterile saline solution containing Gentamicin (3 mg/ml; Henry Schein), heparin (30 USP/ml; Henry Schein), and, for the week following surgery only, streptokinase (9.33 USP/ml; MP Biomedicals).

Bender B.N, & Torregrossa M.M. (2020). Dorsolateral striatum dopamine-dependent cocaine seeking is resistant to Pavlovian cue extinction in male and female rats. Neuropharmacology, 182, 108403.

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Surgical Implantation of Intravenous Catheters in Rats

Cited 1 time

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Surgical implantations of intravenous catheter were done as previously described [34 (link)]. Briefly, we anesthetized the rats with an intraperitoneal injection of ketamine (50 mg/kg) and xylazine (5 mg/kg) and inserted polyurethane catheters (SAI Infusion Technologies, Lake Villa, IL) into the jugular vein. One end of the catheters was in the jugular vein while the other end was attached to modified 22-gauge cannulas that were mounted to the rats’ backs with dental cement to serve as catheter externalized infusion ports. The catheter infusion ports were closed using dust caps (PlasticOne, Roanoke, VA). We used subcutaneous injections of buprenorphine (0.1 mg/kg) after surgery to relieve pain and allowed the rats to recover for 5–7 days before oxycodone self-administration (SA) training. Thereafter, the catheters were flushed every 48 h with gentamicin (0.05 mg/kg, Henry Schein, Melville, NY) and sterile saline to maintain patency.

Blackwood C.A., Hoerle R., Leary M., Schroeder J., Job M.O., McCoy M.T., Ladenheim B., Jayanthi S, & Cadet J.L. (2018). Molecular Adaptations in the Rat Dorsal Striatum and Hippocampus Following Abstinence-Induced Incubation of Drug Seeking After Escalated Oxycodone Self-Administration. Molecular Neurobiology, 56(5), 3603-3615.

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Implantation of Jugular Vein Catheter in Rats

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Surgery for the implantation of intravenous catheter was done essentially as described before ^{26 , 31 (link)}. Rats were anesthetized with ketamine (50 mg/kg, intraperitoneal) and xylazine (5 mg/kg, intraperitoneal) and inserted with silastic catheters into the jugular vein. The catheters were attached to a modified 22-gauge cannula that was mounted to the rats’ skulls with dental cement. We injected buprenorphine (0.1 mg/kg, subcutaneous) after surgery to relieve pain and allowed the rats to recover for 7 days before METH SA training. During the recovery, training and punishment phases, we flushed the catheters every 24–48 h with gentamicin (Henry Schein; 5 mg/ml) and sterile saline.

Cadet J.L., Brannock C., Krasnova I.N., Jayanthi S., Ladenheim B., McCoy M.T., Walther D., Godino A., Pirooznia M, & Lee R.S. (2016). Genome-wide DNA hydroxymethylation identifies potassium channels in the nucleus accumbens as discriminators of methamphetamine addiction and abstinence. Molecular psychiatry, 22(8), 1196-1204.

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Intravenous Catheter Placement and Maintenance

Cited 1 time

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Placement of intravenous catheter was done as described previously (Cadet et al., 2017 (link)). Aseptic techniques were used for surgical procedures. Two weeks after the pre-exposure injections, animals were anesthetized with an intraperitoneal injection of ketamine and xylazine for insertion of polyurethane catheters (SAI Infusion Technologies, Lake Villa, IL) into their jugular veins. Post-surgical subcutaneous injections of buprenorphine (0.05 mg/kg) were given for analgesia. Rats were allowed one week to recover post-surgery prior to the SA experiment. During recovery and throughout the experiment, catheters were flushed every 48 h with gentamicin (5 mg/ml, 0.05 ml, Henry Schein, Melville, NY)-containing sterile saline.

Chojnacki M.R., Jayanthi S, & Cadet J.L. (2020). Methamphetamine pre-exposure induces steeper escalation of methamphetamine self-administration with consequent alterations in hippocampal glutamate AMPA receptor mRNAs. European journal of pharmacology, 889, 173732.

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