Eagle 4k ccd digital camera

Mice were euthanized and perfused with sodium phosphate buffer (PB, 100 mM, pH7.4) and pre-fixed solution (2.5% (vol/vol) glutaraldehyde, 1% paraformaldehyde in PB). Soleus muscle was dissected, cut into small pieces and fixed in the same pre-fixed solution overnight at 4 °C. After rinsing with PB, tissues were immersed in 0.2 M imidazole in PB for 15 min, and then post-fixed with 1% osmium tetraoxide in PB. After rinsing with high-purity water, the samples were stained with 1% aqueous lead at 4 °C overnight. Gradient dehydration was accomplished by incrementing the concentration of acetone and embedded in epoxy resin (60 °C for 24 h). Samples were sectioned using Leica EM UC7 and placed on copper grids. Images were taken on a FEI Tecnai G2 20 Twin electron microscope equipped with an Eagle 4k CCD digital camera (FEI; USA) in a double-blind manner.

Mice were euthanized and perfused with sodium phosphate buffer (PB, 100mM, pH7.4) and pre-fixed solution (2.5% (vol/vol) glutaraldehyde, 1% paraformaldehyde in PB). Tibialis anterior (TA) muscle was dissected, cut into small pieces and fixed in the same pre-fixed solution overnight at 4°C. After rinsing with PB, tissues were immersed in 0.2 M imidazole in PB for 15 minutes, and then post-fixed with 1% osmium tetraoxide in PB. After rinsing with high-purity water, the samples were stained with 1% aqueous lead at 4°C overnight. Gradient dehydration was accomplished by incrementing the concentration of acetone and embedded in epoxy resin (60°C for 24 h). Samples were sectioned using Leica EM UC7 and placed on copper grids. Images were taken on a FEI Tecnai G2 20 Twin electron microscope equipped with an Eagle 4k CCD digital camera (FEI; USA) in a double-blind manner.