L tryptophan d5

Manufactured by Merck Group

Sourced in United States, Netherlands, Sao Tome and Principe

L-tryptophan-d5 is a stable isotope-labeled amino acid. It is a deuterium-labeled version of the essential amino acid L-tryptophan, with five deuterium atoms substituted for hydrogen atoms in the indole ring. This compound is used as a labeled internal standard in analytical methods for the quantification of L-tryptophan in biological samples.

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Lab products found in correlation

4 protocols using l tryptophan d5

HPLC-MS/MS Bioanalysis of Neurotransmitters

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HPLC grade methanol (MeOH), acetonitrile (ACN), and formic acid (FA) were purchased from Merck (Darmstadt, Germany), and pure water was obtained from Wahaha Group Co., Ltd (Hangzhou, China). Other chemicals were of analytical grade. Standard compounds, including l-tryptophan (Trp), l-tyrosine (Tyr), glutamine (Gln), 5-hydroxyindoleacetic acid (5-HIAA), 5-hydroxytryptophan (5-HTP), glutamate (Glu), taurine, l-3,4-dihydroxyphenylalanine (l-DOP), l-asparagine (Asn), 5-hydroxytryptamine (5-HT), acetylcholine (Ach), γ-aminobutyric acid (GABA), kynurenine, and ten stable isotope-labeled internal standards: γ-aminobutyric acid-d₂, choline-d₉, 5-hydroxytryptamine-d₄, taurine-¹⁵N, indoleacetic acid-d₂, glutamine-¹⁵N, glutamate-d₅, l-tryptophan-d₅, l-tyrosine-d₂, l-asparagine-d₁ were purchased from Sigma-Aldrich (St. Louis, MO, USA), and olanzapine (OLZ) bulk drug were purchased from Shanghai Yuan Ye Biotechnology Co., Ltd.

Liu D., An Z., Li P., Chen Y., Zhang R., Liu L., He J, & Abliz Z. (2020). A targeted neurotransmitter quantification and nontargeted metabolic profiling method for pharmacometabolomics analysis of olanzapine by using UPLC-HRMS. RSC Advances, 10(31), 18305-18314.

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LC-MS Metabolite Quantification Standards

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MS-grade acetonitrile (ACN) and methanol (MeOH) were purchased from Thermo Fisher Scientific, USA. Formic acid was obtained from DIMKA, and ammonium formate was purchased from Fluka (Honeywell Fluka, USA). Ultrapure water was filtered through the Milli-Q system (Millipore, Billerica, MA). The internal standard (IS) mixture contained L-Leucine-d3 (Toronto Research Chemicals, TRC), L-Phenylalanine (¹³C9, 99%) (Cambridge Isotope Laboratories, CIL), L-Tryptophan-d5 (TRC), Progesterone-2,3,4-¹³C3 (Sigma).

Bai Y., Zhang H., Wu Z., Huang S., Luo Z., Wu K., Hu L, & Chen C. (2022). Use of ultra high performance liquid chromatography with high resolution mass spectrometry to analyze urinary metabolome alterations following acute kidney injury in post-cardiac surgery patients. Journal of Mass Spectrometry and Advances in the Clinical Lab, 24, 31-40.

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Quantifying IDO1 Activity in CLL Cells

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To assess the effect of IFN-γ on IDO1 activity, purified CD19⁺ CLL cells were resuspended in 0.5 ml of RPMI-1640 medium + 10% FBS and cultured at a density of 12 × 10⁶/well in 12-well plates. Cells were pretreated (or not) with INCB018424 1 µM for 1 h before the incubation with IFN-γ for 24 h. Similarly, 0.5 ml of conditioned media was collected from 5 × 10⁶ CLL cells 24 h post transfection with the IDO1 vector, or the corresponding empty vector. Conditioned media were collected by centrifugation at 2,000 × g for 15 min and stored at −80°C until assayed. Fifty µl of these supernatants was added with an equal volume of ice-cold 1 M perchloric acid (HClO₄) fortified with a mix of the following stable isotope-labeled internal standard (final concentration 1 µM): L-kynurenine-d4 (Buchem BV, Apeldoorn, Netherlands) and L-tryptophan-d5 (Sigma-Aldrich). Samples were centrifuged (15,000 × g, 15 min), and the supernatants were collected and directly injected into LC-MS/MS.

Atene C.G., Fiorcari S., Mesini N., Alboni S., Martinelli S., Maccaferri M., Leonardi G., Potenza L., Luppi M., Maffei R, & Marasca R. (2022). Indoleamine 2, 3-Dioxygenase 1 Mediates Survival Signals in Chronic Lymphocytic Leukemia via Kynurenine/Aryl Hydrocarbon Receptor-Mediated MCL1 Modulation. Frontiers in Immunology, 13, 832263.

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Deuterated Standards for Metabolomics

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Citric acid-d₄, L-leucine-d₁₀, D-fructose -¹³C₆, L-tryptophan-d₅, L-valine-d₃, glycerol-d₃, benzoic acid-d₅, stearic acid-d₃₅, Norvaline, HPLC grade acetone and HPLC grade methanol were all purchased from Sigma Aldrich (St. Louis, MO). L-Alanine-d₄ was purchased from Isotec (Miamisburg, OH). Optima grade acetonitrile was purchased from Fisher Scientific (Fair Lawn, NJ).
Two solutions were prepared to serve as internal standards using all deuterated compounds. The first solution, deuterated standard solution, contained all compounds except for stearic acid-d₃₅ at a concentration of 15 ng/μL dissolved in 50:50 MeOH:dH₂O. The second solution contained stearic acid-d₃₅ at a concentration of 60 ng/μL dissolved in acetone. A reconstitution solution was prepared as 4:1 acetonitrile:H₂O (milliQ) with Norvaline added at a concentration of 1.5 ng/μL.

Goldberg E., Ievari-Shariati S., Kidane B., Kim J., Banerji S., Qing G., Srinathan S., Murphy L, & Aliani M. (2021). Comparative metabolomics studies of blood collected in streck and heparin tubes from lung cancer patients. PLoS ONE, 16(4), e0249648.

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