Solvents used for synthesis (dichloromethane, ethyl acetate) were purchased from Lach-ner s.r.o. Tetrahydrofuran was dried with sodium and freshly distilled before use in the presence of benzophenone. Acetone was dried with calcium(II)chloride, distilled from phosphorus(V)oxide, and stored above molecular sieves. Chemicals used for synthesis were purchased from Merck KGaA (Darmstadt, Germany) and Fluorochem Ltd. (Hadfield, UK). Deuterated methanol (MeOD-d4, min. 99.8% D) for vibrational circular dichroism (VCD) analysis was purchased from ISOSAR GmbH (Saarbrücken, Germany) and methanol for electron circular dichroism (ECD) analysis with the purity of 99.8% from Sigma-Aldrich Inc. (St. Louis, MO, USA). Hexane, heptane, and propan-2-ol (2-PrOH) for liquid chromatography were purchased from Honeywell (Charlotte, NC, USA), ethanol (EtOH) was obtained from Fischer Scientific (Hampton, NY, USA), and diethylamine (DEA) from Merck KGaA (Darmstadt, Germany).
Ethanol etoh
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom, United States
Ethanol (EtOH) is a clear, colorless liquid compound commonly used in various laboratory applications. It serves as a solvent, disinfectant, and fuel source. Ethanol has a distinct alcohol-like odor and is highly flammable. It is a versatile chemical that can be utilized in a wide range of laboratory procedures and experiments.
Automatically generated - may contain errors
Lab products found in correlation
Acetonitrile acn, by Thermo Fisher Scientific (3 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Ethyl acetate etoac, by Merck Group (2 mentions)
Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (2 mentions)
Hydrochloric acid (hcl), by Thermo Fisher Scientific (2 mentions)
Sodium hydroxide (naoh), by Merck Group (2 mentions)
Oleic acid, by Merck Group (2 mentions)
Methanol, by Merck Group (1 mentions)
Hexane, by Honeywell (1 mentions)
Dichloromethane, by Lach-Ner (1 mentions)
Heptane, by Honeywell (1 mentions)
Cremophor el, by Merck Group (1 mentions)
Methanol meoh, by Avantor (1 mentions)
Distilled water, by Tedia (1 mentions)
Tert butanol, by Merck Group (1 mentions)
Dimethylacetamide dma, by Merck Group (1 mentions)
Genistein, by LC Laboratories (1 mentions)
Formic acid, by Biosolve (1 mentions)
Water of uhplc ms grade, by Thermo Fisher Scientific (1 mentions)
Astaxanthin, by Merck Group (1 mentions)
23 protocols using ethanol etoh
1
Synthesis and Characterization of Compounds
2
Formulation and Characterization of Polymer-Based Drug Delivery Systems
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Methoxy poly(ethylene glycol)-b-poly(D,L-lactide) (mPEG [4000]-b-PLA [2200]) was purchased from Advanced Polymer Materials, Inc. (Montreal, QC, Canada). The mPEG-b-PCL (MW 2000:2000 Da) was purchased from PolySciTech® (West Lafayette, IN, USA). Soluplus® [Polyvinyl caprolactam-polyvinyl acetate-polyethylene glycol graft copolymer (PCL-PVAc-PEG)] was obtained from BASF (Ludwigshafen, Rhineland-Palatinate, Germany). RAPA and genistein were purchased from LC Laboratories® (Woburn, MA, USA). FEN, dimethyl sulfoxide (DMSO), thiazolyl blue tetrazolium bromide (MTT), tert-butanol, cremophor EL®, and dimethylacetamide (DMA) were purchased from Sigma-Aldrich Corp. (St. Louis, MO, USA). Distilled water (DW) was purchased from Tedia (Fairfield, OH, USA). Acetonitrile (ACN) and ethanol (EtOH) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Methanol (MeOH) was purchased from Honeywell, Burdick & Jackson (Ulsan, Korea). All other reagents were used as received without additional purification and were at least HPLC grade.
3
Quantification of Phytocannabinoids by LC-MS
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The phytocannabinoid standard solutions at 1 mg mL -1 in ethanol (for THC) and in 2-isopropanol (for THCA-A) used for this study were purchased from Lipomed AG (Arlesheim, Switzerland). Ethanol (EtOH) and acetonitrile (ACN) of OPTIMA LC-MS grade, as well as water of UHPLC-MS grade, were purchased from Fischer Scientific (Loughborough, UK). Formic acid was obtained from Biosolve (Valkenswaald, Netherlands).
4
Carotenoid Extraction and Quantification
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All solvents used for the extractions and separations were of analytical grade. Specifically, ethanol (EtOH) was purchased from Acros Organics, methanol (MeOH), hexane and petroleum ether were provided by Carlo Erba, while isopropanol (IPA) and methyl-tert-butyl-ether (MTBE) were obtained from Fisher Chemicals. The HPLC grade solvents acetonitrile (ACN), chloroform and dichloromethane (DCM) were also obtained from Fisher Chemicals. Water, acetonitrile and methanol used for the LC–MS/MS determinations were obtained from J.T. Baker as LC-MS grade solvents. Finally, dimethyl sulfoxide (DMSO), potassium hydroxide (KOH), ethyl acetate (EtOAc) and diethyl ether (Et2O) were purchased from Sigma-Aldrich. Silica gel 60 (230–400 mesh) was obtained from Merck.
Zeaxanthin (≥98%), lutein (≥95%), and β-cryptoxanthin (≥97%) standards were purchased from Extra Synthese; (E/Z) phytoene (≥95%) and α-carotene (≥95%) standards were obtained from CaroteNature. Astaxanthin (≥95%), lycopene (≥95%), α-carotene (≥95%) and fucoxanthin (≥95%) standards were provided by Sigma-Aldrich. These carotenoid standards were selected as the most significant carotenoids in different matrices, such as plant material, animal feed, aquafeed and various tissues.
Zeaxanthin (≥98%), lutein (≥95%), and β-cryptoxanthin (≥97%) standards were purchased from Extra Synthese; (E/Z) phytoene (≥95%) and α-carotene (≥95%) standards were obtained from CaroteNature. Astaxanthin (≥95%), lycopene (≥95%), α-carotene (≥95%) and fucoxanthin (≥95%) standards were provided by Sigma-Aldrich. These carotenoid standards were selected as the most significant carotenoids in different matrices, such as plant material, animal feed, aquafeed and various tissues.
5
Aptamer Selection and Protein Binding Assays
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Dithiothreitol (DTT), NaCl, MgCl2, 1× PBS buffer, glacial acetic acid, hydrochloric acid, DMSO, titanium (IV) isopropoxide, guanidine HCl (Gu-HCl) and potassium chloride (KCl) were obtained from Fisher Scientific. Polyvinylpyrrolidone (PVP) was acquired from Sigma-Aldrich. Tris base, EDTA and ethanol (EtOH) was obtained from Acros Organics, Promega and Decon Labs, respectively. The 5× Taq polymerase and the 25-mM magnesium chloride (MgCl2) solution used for PCR were from New England Biolabs. The ssDNAs, including the random libraries used for SELEX, the anti-IgE aptamer, as well as those found binding to DNMT1 (Supplementary Tables S1 and S3 ) were attained from Integrated DNA Technologies, Inc. Beta-casein, cytochrome C, ovalbumin, and hemoglobin were purchased from Sigma-Aldrich. IgE was acquired from Athens Research & Technology, Inc.
6
Platinum-based Nanoparticle Synthesis and Characterization
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Cur (Purity: >75%), Pluronic F-127, [3-(2-aminoethylamino)propyl]trimethoxysilane (Purity: 97%), glutaric anhydride (GA, Purity: 95%), cis-diamine dichloro-platinum (II) (Purity: 99.9%), 1,3-diphenylisobenzofuran (DPBF, Purity: 97%), trypan blue solution (0.4%), o-phenylenediamine (OPDA, Purity: ≥98.0%), ninhydrin, and Thiazolyl Blue Tetrazolium Bromide (MTT, Purity: 98%) were purchased from Sigma Aldrich Co. Ltd. (St. Louis, MO, USA). Dimethyl sulfoxide (DMSO, Purity: ≥99.9%), dimethylformamide (DMF Purity: ≥99.9%), ethanol (EtOH), and toluene (Purity: 99%) were obtained from Acros Organics (Loughborough, UK). JC-1 assay kit was purchased from Invitrogen (Waltham, MA, USA).
7
Electrochemical Aptamer Biosensor Synthesis
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All reagents and solvents were used without further purification. 3,4-dimethoxythiophene was purchased from Matrix scientific. 3,4-Ethylenedioxythiophene (EDOT), 3-chloro-1,2-propanediol, p-toluene sulfonic acid monohydrate, toluene, tetrahydrofuran (THF), hexane, 3-mercaptopropionic acid (MPA), 2,2-Dimethoxy-2-phenylacetophenone (DMPA), Sodium Hydroxide (NaOH), Potassium hydroxide (KOH), Tris(2-carboxyethyl) phosphine hydrochloride (TCEP), Methanol (MeOH) and dichloromethane were purchased from Sigma. Potassium tert-butoxide (tBuOK) was purchased from ACROS. Ethanol (EtOH) was purchased through Fisher. Aptamer 5′-HS-(CH2)6-AGACAAGGAAAATCCTTCAATGAAGTGGGTCG-(CH2)7-MB-3′ with a 5′ thiol group as covalent linkage site and 3′ methylene blue (MB) as electrochemical reporter was purchased through Biosearch Technologies, Inc., Platinum/Iridium wires were purchased from A-M systems.
8
Antioxidant Compound Sourcing and Characterization
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Caftaric acid, 2,2-azino-bis-3-ethylbenzthioazolin-6-sulfonic acid, Trolox, butylated hydroxytoluene, 2,2-diphenyl-1-picrylhydrazyl, fluorescein, 2,2′-azobis(2-amidinopropane)dihydrochloride (AAPH), disodium phosphate, and sodium chloride were purchased from Sigma-Aldrich (Saint-Quentin-Fallavier, France). Potassium chloride and potassium persulfate were purchased from VWR (Fontenay-sous-Bois, France), and potassium dihydrogen phosphate was purchased from Merck (Darmstadt, Germany). Isoquercitrin, rutin, luteolin-7-O-glucoside, isorhamnetin-3-O-rutinoside, and apigenin-7-O-glucoside were purchased from Extrasynthese (Genay, France).
Ultrapure water (18.2 MΩ‧cm) was used for extraction and chromatography and was prepared using a Millipore Synergy system (Molsheim, France). Hydrochloric acid, acetonitrile (ACN), ethyl acetate, and ethanol (EtOH) were purchased from Fisher Scientific (Illkirch-Graffenstaden, France). Methanol (MeOH) and chloroform were obtained from Carlo Erba (Val-de-Reuil, France). Formic acid was purchased from Sigma-Aldrich (Saint Quentin Fallavier, France).
Ultrapure water (18.2 MΩ‧cm) was used for extraction and chromatography and was prepared using a Millipore Synergy system (Molsheim, France). Hydrochloric acid, acetonitrile (ACN), ethyl acetate, and ethanol (EtOH) were purchased from Fisher Scientific (Illkirch-Graffenstaden, France). Methanol (MeOH) and chloroform were obtained from Carlo Erba (Val-de-Reuil, France). Formic acid was purchased from Sigma-Aldrich (Saint Quentin Fallavier, France).
9
HPLC-Grade Solvent Acquisition
10
Synthesis of Gold Nanoparticles
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Gold salt (HAuCl4·3H2O), trisodium citrate dihydrate, N,N’-methylene-bis-acrylamide (BIS), ammonium persulfate (APS), potassium carbonate (K2CO3), phenylboronic acid, cetyltrimethylammonium bromide solution (CTAB), L-ascorbic acid (ASA), and ethanol (EtOH) were purchased from Fisher Scientific. N-isopropylacrylamide (NIPAM) was purchased from Sigma-Aldrich and recrystallized in hexanes prior to use. Water used in all reactions was obtained from a Nanopure water system equipped with a 0.2 µm membrane filter (Barnstead/Thermolyne). All glassware was cleaned with a strong acid (3:1 ratio of HCl:HNO3) and a base (KOH in isopropanol).
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