Ire1α 14c10

Manufactured by Cell Signaling Technology

Sourced in United States

IRE1α (14C10) is a rabbit monoclonal antibody that recognizes the endoplasmic reticulum (ER) stress sensor, Inositol-Requiring Enzyme 1α (IRE1α). The core function of IRE1α is to initiate the unfolded protein response (UPR) pathway in cells experiencing ER stress.

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Lab products found in correlation

Bip c50b12, by Cell Signaling Technology (2 mentions) Tomm20, by Merck Group (1 mentions) Gapdh, by Abcam (1 mentions) Parkin, by Abcam (1 mentions) Cox 4, by Abcam (1 mentions) Traf2, by Abcam (1 mentions) Facl4, by Abcam (1 mentions) α sarcomeric actin, by Abcam (1 mentions) Ab11433, by Abcam (1 mentions) Goat anti rabbit igg hrp, by Santa Cruz Biotechnology (1 mentions) Goat anti mouse igg hrp, by Santa Cruz Biotechnology (1 mentions) β actin km9001t, by Sungene Biotech (1 mentions) P eif2α, by Cell Signaling Technology (1 mentions) Anti mouse 7076, by Cell Signaling Technology (1 mentions) Ripa buffer, by Merck Group (1 mentions) Mitosox, by Thermo Fisher Scientific (1 mentions) Annexin 5 binding buffer, by Thermo Fisher Scientific (1 mentions) Propidium iodide, by Merck Group (1 mentions) Fitc conjugated annexin 5, by Thermo Fisher Scientific (1 mentions) Er tracker green, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

IRE1α (166 citations) Rabbit anti-IRE1α (14C10) (2 citations)

4 protocols using ire1α 14c10

Immunoblotting Techniques for Cellular Protein Analysis

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Immunoblotting was performed as previously described.²⁵ Specific antibodies employed are as follows: LC3 (Novus Biologicals, NB100-2220); p62 (Abcam, ab56416); TRAF2 (Abcam, ab126758); COX IV (Abcam, ab14744); TOMM20 (Sigma, WH0009804M1); VDAC (Cell Signaling Technology, 4661S); PARKIN (Abcam, ab15954); FACL4 (Abcam, ab155282); calreticulin Antibody #2891; VAPB (Thermo Fisher Scientific, A302-894A); IRE1α (14C10) (Cell Signaling Technology, 3294S); GAPDH (Abcam, ab22555); TLR9 (Novus Biologicals, NBP2-24729); actin (Sigma, A2066); PINK1 (MRC PPU products and reagents, S774C [DU17570] and S086D [DU34559]); and α-sarcomeric actin (Abcam, ab52219).

Ma X., Rawnsley D.R., Kovacs A., Islam M., Murphy J.T., Zhao C., Kumari M., Foroughi L., Liu H., Qi K., Diwan A., Hyrc K., Evans S., Satoh T., French B.A., Margulies K.B., Javaheri A., Razani B., Mann D.L., Mani K, & Diwan A. (2021). TRAF2, an Innate Immune Sensor, Reciprocally Regulates Mitophagy and Inflammation to Maintain Cardiac Myocyte Homeostasis. JACC: Basic to Translational Science, 7(3), 223-243.

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Molecular Markers of UPR Activation

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NMS‐873 (purity >98%) was purchased from Dalian Meilun Biotechnology (CAS: 1418013‐75‐8), the compound was dissolved in DMSO and stored at 4°C. Primary Abs used for the western blot analysis included inositol‐requiring enzyme 1α (IRE1α) (14C10; Cell Signaling Technology), X‐box binding protein 1 (XBP‐1(s)) (D2C1F; Cell Signaling Technology), phospho‐protein kinase RNA‐like ER kinase (p‐PERK) (DF7576; Affinity Biotechnology), phospho‐eukaryotic initiation factor 2α (p‐eIF2α) (119A11, Cell Signaling Technology), activating transcription factor 6 (ATF6) (D4Z8V; Santa Cruz Biotechnology), binding immunoglobulin protein (BiP) (C50B12; Cell Signaling Technology), and C/EBP homologous protein (CHOP) (D46F1; Cell Signaling Technology). Antibody to detect VCP was purchased from Abcam (ab11433), the GAPDH (KM9002T) and β‐actin (KM9001T) were obtained from Sungene Biotech. Secondary Ab included goat anti‐mouse IgG‐HRP (sc‐2005) and goat anti‐rabbit IgG‐HRP (sc‐2004) were from Santa Cruz Biotechnology.

Luo H., Song H., Mao R., Gao Q., Feng Z., Wang N., Song S., Jiao R., Ni P, & Ge H. (2019). Targeting valosin‐containing protein enhances the efficacy of radiation therapy in esophageal squamous cell carcinoma. Cancer Science, 110(11), 3464-3475.

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Mitochondrial Dysfunction and ER Stress Assay

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Tunicamycin (Tuni, #sc-3506) and JC-1 (#sc-364116A) were purchased from Santa Cruz Biotechnology. RIPA Buffer (#R0278), Protease Inhibitor Cocktail (PIC, #P8340), Propidium Iodide (PI, #P4864) were purchased from Sigma; 3- [4, 5-dimethylthiazol-2-yl]− 2,5-di-phenyltetrazolium bromide (MTT, #33611) was obtained from SRL; beta-cyclodextrin (β-CD, #C0900), and 3-(2-Benzothiazolyl)− 7-(diethylamino), coumarin (C6, #B2088) were purchased from TCI Chemicals. MitoSOX (#M36008), Fura-2 AM cell permeant(#F1221), ER Tracker Green (#E34251) was purchased from Invitrogen. FITC conjugated AnnexinV (#A13199), AnnexinV binding buffer (#V13246), Enhanced Chemiluminescence (ECL, #32106) and Antifademountant (4′-6-diamidino-2-phenylindole, #P36962) were procured from Thermo Fisher Scientific. Antibodies (TOM20 #D8T4N, DRP1 #D6C7, phospho-DRP1 #D9A1, IRE1α #14C10, BiP #C50B12, Calnexin #C5C9) were obtained from Cell Signaling Technology (CST, USA) and Santa Cruz Biotechnology (β-actin #sc-69879, GAPDH #sc-365062) and secondary antibodies- (anti-mouse #7076 S and anti-rabbit #7074P2) were procured from Cell Signaling Technology.

Dey S., Fageria L., Sharma A., Mukherjee S., Pande S., Chowdhury R, & Chowdhury S. (2022). Silver nanoparticle-induced alteration of mitochondrial and ER homeostasis affects human breast cancer cell fate. Toxicology Reports, 9, 1977-1984.

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Evaluating Notch1 and EGFR Signaling

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NXD30001 was obtained from NexGenix Pharmaceuticals, 17AAG was generously provided by Kosan Bioscience, Inc. The antibodies used in this study including Phospho(p)-EGFR (1H12, #2236), EGFR (D6B6, #2085), pS473-Akt (D9E, #4060), Akt (40D4, #2920), Cleaved-Notch1 (D3B8, #4147), Notch1 (D1E11, #3608), c-Myc (D84C12, #5605), p-MEK (Thr286, #9127), p-ERK1/2 (D13.14.4E, #4370), ERK1/2 (L34F12, #4696), p-ATR (Ser428, #2853), p-CHK2 (Thr68, #2661), p-p53 (Ser15, #9286), p-H2AX (D7T2V, #80312), PERK (D11A8, #5683), IRE1α (14C10, #3294), BiP (C50B12, #3177), and CHOP (L63F7, #2895) were purchased from Cell signaling Technology. Mouse monoclonal antibody against actin (#MAB1501) was purchased from Millipore. Secondary antibodies were obtained from Santa Cruz Biotechnology, Inc.

Chen H., Gong Y., Ma Y., Thompson R.C., Wang J., Cheng Z, & Xue L. (2020). A Brain-Penetrating Hsp90 Inhibitor NXD30001 Inhibits Glioblastoma as a Monotherapy or in Combination With Radiation. Frontiers in Pharmacology, 11, 974.

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