Tunel apoptosis detection kit

Manufactured by RiboBio

The TUNEL apoptosis detection kit is a laboratory tool used to detect and quantify apoptosis, a type of programmed cell death, in biological samples. The kit utilizes the TUNEL (Terminal deoxynucleotidyl transferase dUTP Nick End Labeling) assay, which labels the fragmented DNA that is characteristic of apoptotic cells. This allows for the identification and analysis of apoptotic cells through various detection methods.

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Lab products found in correlation

Cm1850, by Leica camera (1 mentions) Mitosox reagent, by Thermo Fisher Scientific (1 mentions) Gsh gssg detection kit, by Beyotime (1 mentions) Nadp nadph detection kit, by Beyotime (1 mentions) Rhod 2, by Abcam (1 mentions) Annexin 5 fitc pi staining, by BD (1 mentions) Bcl 2, by Abcam (1 mentions) Caspase 3, by Abcam (1 mentions) Atp detection kit, by Beyotime (1 mentions) Jc 1 mitochondrial membrane potential detection kit, by Beyotime (1 mentions)

3 protocols using tunel apoptosis detection kit

PC12 Cell Apoptosis Assay

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A total of 1x10⁴ PC12 cells/well were made into a suspension and stained with 50 µg/mL Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) in the dark for 15 min. Then, the cell apoptosis was determined using an Annexin V apoptosis kit I (BD Biosciences) and a flow cytometer (Becton Dickinson).
TUNELWell-growing PC12 cells or the rat brain tissue sections were washed with D-Hank three times, and then the apoptosis of cells was further washed using a TUNEL apoptosis detection kit (Guangzhou RiboBio Co., Ltd.) as per the manufacturer’s protocol. A total of five randomly selected areas were observed under the microscope, under which the TUNEL-positive cells present greenery nuclei. All procedures were performed in triplicate.

Bu X., Li D., Wang F., Sun Q, & Zhang Z. (2020). Protective Role of Astrocyte-Derived Exosomal microRNA-361 in Cerebral Ischemic-Reperfusion Injury by Regulating the AMPK/mTOR Signaling Pathway and Targeting CTSB. Neuropsychiatric Disease and Treatment, 16, 1863-1877.

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Apoptosis Detection in Retinal Sections

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The eyeballs were fixed in 4% PFA at room temperature for 12 h, immersed in 30% sucrose solution for 12 h, and embedded in optimum cutting temperature compound. The retinas were then enucleated and sectioned at 10-μm thickness using a cryostat (Leica CM1850). For each eye, 3–4 serial sections were taken with a step of 50 μm. The apoptosis of retinal sections was detected by a TUNEL Apoptosis Detection Kit (Ribobio, Guangzhou, China) according to the manufacturer’s protocols. Briefly, retinal sections were incubated with the blocking buffer (3% H₂O₂ in CH₃OH) for 30 min at room temperature. After washing with PBS three times, retinal sections were incubated with TUNEL reaction mixture for 1 h at 37°C. Following washing with PBS three times, DAPI was added for 1 min to label nuclei. The slides were mounted and examined by a fluorescence microscope.

Wang T., Li S., Li X.M., Li C., Wang F, & Jiang Q. (2023). Targeting circular RNA-Glra2 alleviates retinal neurodegeneration induced by ocular hypertension. Aging (Albany NY), 15(19), 10705-10731.

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Comprehensive Analysis of Mitochondrial Function

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Step RT-qPCR Kit (TIANGEN) was used to detect MCU mRNA expression, and Western blot was used to detect MCU protein expression. JC-1 Mitochondrial membrane potential detection kit (Beyotime) detects mitochondrial membrane potential, ATP detection kit (Beyotime) detects cardiomyocyte mitochondrial ATP activity, mitochondrial calcium dye Rhod-2 (Abcam) was used to detect myocardial cell mitochondrial calcium uptake capacity, and Mito-SOX reagent (Thermo Fisher Scientific) was used to detect Changes of reactive oxygen species (ROS) in cardiac mitochondria. NADP + /NADPH detection kit (Beyotime) and GSH/GSSG detection kit (Beyotime) were used to detect myocardial cells NADP + /NADPH, GSH/GSSG. Western blot was used to detect the expression of caspase-3 (Abcam), cleared caspase-9 (Abcam) and Bcl-2 (Abcam). Flow cytometry combined with Annexin V-FITC/PI staining (BD) and TUNEL apoptosis detection kit (RIBO) was used to detect cardiomyocyte apoptosis.

Chen X., Guo W., Jing Z., Zhang T., Wu Z., Cui H., Zheng W., Chen Y., & Chen H. (2020). The mitochondrial calcium uniporter induces apoptosis in cardiomyocytes cultured with high-glucose medium by affecting mitochondrial function.

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