Animal experiments were performed as described previously [49 (link)]. Briefly, BALB/c-nu mice (female, 6–8 weeks old; Orient Bio, Seongnam, South Korea) were used to establish a tumor xenograft model. NTERA-2 Tet-on-shOCT4 OCT WT- and S236D-rescued cells (5.0 × 106/each in opposite flanks) were inoculated subcutaneously. After 4–5 weeks, when the tumor sizes reached 50–100 mm3, the mice were randomly divided into two groups, a control group and a Dox group. Dox (1 mg/mL, #631311; Clontech Laboratories, Mountain View, CA, USA) was fed 5% sucrose drinking water. The primary tumor size and body weight were measured every week using calipers and a balance, respectively. The tumor volume was calculated using the formula V = (A × B2)/2, where V is the volume (mm3), A is the long diameter, and B is the short diameter. Mice were killed in a 7.5% CO2 chamber, and the tumors were harvested for further analysis. All mice were attended under specific pathogen-free conditions at the National Cancer Center Research Institute Animal Facility. This study was reviewed and approved by the Institutional Animal Care and Use Committee of the National Cancer Center Research Institute (NCC-17-401), an Association for Assessment and Accreditation of Laboratory Animal Care International-accredited facility.
Balb c nu mice
Manufactured by Orient Bio
BALB/c-nu mice are a strain of nude mice, which are immunodeficient and lack a functional thymus. These mice are commonly used in biomedical research due to their inability to reject xenografts, making them useful for the study of human tumor growth and the evaluation of novel cancer therapies.
Automatically generated - may contain errors
Lab products found in correlation
Male sprague dawley rats, by Orient Bio (2 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions)
Pbs matrigel, by BD (1 mentions)
Anti alpha smooth muscle actin α sma, by Agilent Technologies (1 mentions)
Anti human cd31, by Santa Cruz Biotechnology (1 mentions)
Target retrieval solution, by Agilent Technologies (1 mentions)
Matrigel, by BD (1 mentions)
7 protocols using balb c nu mice
1
Xenograft Tumor Model for OCT4 Regulation
2
Synergistic anti-tumor effects of gossypol and phenformin
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Balb/c-nu mice (Orientbio, Seongnam, Korea), aged between 6 and 8 weeks before tumor induction, were used for this model. This study was reviewed and approved by the Institutional Animal Care and Use Committee of the National Cancer Center Research Institute, which is an Association for Assessment and Accreditation of Laboratory Animal Care International (AAALAC International) accredited facility that abides by the Institute of Laboratory Animal Resources guide (protocols: NCC-17-397). SNU-638 cells (1.5 × 107) and SK4 cells (1 × 107) in 100 μL PBS were inoculated subcutaneously into mice using a 1 mL syringe. After 1 week, mice were divided into four groups: a control group treated with vehicle only, groups treated with gossypol or phenformin, and a group treated with both gossypol and phenformin. Vehicle (5% DMSO and 5% Cremophor in PBS; 100 μL) alone, gossypol (80 mg/kg/100 μL), and phenformin (100 mg/kg/100 μL) were administered orally once per day, 6 days/week, for 49 days (n = 6, mice per group). The size of the primary tumor was measured every week using calipers. Tumor volume was calculated using the formula, V u (A × B2)/2, where V is the volume (mm3), A is the long diameter, and B is the short diameter.
3
Xenograft Mouse Model for Colorectal Cancer
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In vivo animal studies were approved by the Animal Care Committee of Korea Atomic Energy Research Institute (KAERI-IACUC-2016-030), and all animal care was performed in accordance with the guidelines of the Korea Council on Animal Care. HCT116 xenografts were prepared using 5-week-old female BALB/c-nu mice (15±2 g) obtained from Orient Bio, Inc. (Seongnam, Korea). The mice were housed at 2–3 animals/cage under conditions of controlled temperature (22°C±2°C), humidity (55%±15%), and lighting (illuminance of 150–300 Lux). To generate xenografts, 1×107 HCT119 cells/mL suspended in 200 μL of 1:1 (v/v) PBS/Matrigel (Becton Dickinson Biosciences, Franklin Lakes, NJ, USA) were injected into the flanks of the mice. After injecting the tumor cells, the subcutaneous tumor volume (V) was measured with digital calipers and calculated using the formula V = (ab2)/2, where a is the biggest orthogonal tumor diameter and b is the smallest. When the tumor diameters reached 4–6 mm, the indicated polyplex samples were delivered by subcutaneous injection into the tumor masses. The tumor size was monitored daily for 20 days. Histological observations were performed by H&E and immunohistochemical staining, according to standard procedures.
4
Evaluating Anti-Tumor Effects of G-Rh2 in Xenograft Mice
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BALB/c-nu mice (male, aged 5–6 weeks) were purchased from Orient Bio Inc. (Seongnam, Gyeonggi, Korea). All animal experiments were performed under protocols approved by and accordance with the guidelines of the Kyungpook National University Animal Use and Care Committee (2015-0135). HCT116 cells were collected in PBS at a final concentration of 1 × 107 cells/mL. The cells (200 μL) were subcutaneously injected into the flanks of mice. Twelve days after injection, the mice were randomly divided into three groups (n = 6 mice/group) and treated with an intraperitoneal injection of G-Rh2 (10 mg/kg and 50 mg/kg) or vehicle (PBS) three times a week for 21 days. For Axl knockdown xenograft assays, the mice were randomly divided into two groups (n = 4 mice/group) and sh-Mock or sh-Axl#2 HCT116 cells (5 × 106 cells in 0.2 mL PBS) were subcutaneously injected into the flanks of each mouse. Tumor volumes and body weights were recorded every 4 days. Tumor volumes were assessed using the following formula: (length × width × height × 0.52).
5
Islet Transplantation in Nude Mice
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Male Sprague-Dawley rats (8 weeks, 200–250 g) and BALB/c-nu mice (8 weeks, 20–25 g) purchased from Orient Bio (Gyeonggi-do, Korea) were used as donors and recipients, respectively. In our study, rats were selected as the animal donors because they provide a higher yield of islet. In addition, we used congenitally athymic nude mice as recipients to avoid any interference of immune rejection on the outcome of results.
6
Angiogenic Potential of HUVEC and ahMNC Co-cultures
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Cells (total 2 × 106) were suspended in 200 µL of ice-cold phenol red-free Matrigel (BD Bioscience, Franklin Lakes, NJ, USA) at ratios of 100:0, 50:50, and 0:100 (HUVECs:ahMNCs). The mixtures were then transplanted subcutaneously into the dorsal surface of Balb-c/nu mice (6-week-old, male) (Orient Bio, Seongnam, Korea) in accordance with previous reports [23 (link),47 (link),48 (link)]. At 4 days after injection, the Matrigel plugs were recovered, fixed with 10% buffered formalin, and then embedded in paraffin to prepare 4-µm-thick sections. For histological analysis, H&E staining was applied. For immunofluorescence, deparaffinized and rehydrated sections were incubated in target retrieval solution (Dako, Carpentaria, CA, USA) at 125 °C for 30 min. Anti-human CD31 (Santa Cruz Biotechnology) and anti-alpha smooth muscle actin (αSMA) (Dako) antibody were applied to slides at 4 °C overnight. Nuclei were counter-stained by DAPI (1:1000, ThermoFischer Scientific, Carlsbad, CA, USA) for 5 mins at RT.
7
Xenograft Transplantation Procedure
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Male Sprague-Dawley rats and BALB/c-nu mice purchased from Orient Bio (Seongnam, Korea) were used as donors and recipients, respectively. All animal experiments were approved by the Institutional Animal Care and Use Committee of Asan Medical center (IACUC-2014-13-217).
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