Phenylmethylsulfonyl fluoride

Manufactured by Selleck Chemicals

Sourced in United States

Phenylmethylsulfonyl fluoride is a colorless crystalline solid used as a serine protease inhibitor in laboratory settings. It irreversibly binds to and inhibits the activity of serine proteases, enzymes that play a crucial role in various biological processes.

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Lab products found in correlation

Anti vegfc, by Proteintech (1 mentions) Anti fasn, by Proteintech (1 mentions) Anti soat1, by Abcam (1 mentions) Anti srebp1, by Proteintech (1 mentions) Anti srebp2, by Proteintech (1 mentions) Anti gapdh, by Proteintech (1 mentions) Anti hnrnpa2b1, by Proteintech (1 mentions) Anti gapdh, by Beyotime (1 mentions)

2 protocols using phenylmethylsulfonyl fluoride

Comprehensive Western Blot Procedure

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The Western blot protocol was performed as previously described(Wang et al., 2020 (link)). The antibodies used were listed as follows: anti-SOAT1 (Immunoway, YN1370); anti-SOAT1 (Abcam, ab39327); anti-VEGFC (Proteintech, 14517-1-AP); anti-FASN (Proteintech, 10624-2-AP); anti-SREBP1 (Proteintech, 66875-1-AP); anti-SREBP2 (Proteintech, 28212-1-AP); and anti-GAPDH (Proteintech, 60004-1-Ig). Phenylmethylsulfonyl fluoride was purchased from Selleck (Houston, TX, United States).

Zhu T., Wang Z., Zou T., Xu L., Zhang S., Chen Y., Chen C., Zhang W., Wang S., Ding Q, & Xu G. (2021). SOAT1 Promotes Gastric Cancer Lymph Node Metastasis Through Lipid Synthesis. Frontiers in Pharmacology, 12, 769647.

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Western Blot Antibody Validation

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Western blot assays were performed as previously reported (23 (link)). The following antibodies were used: anti-GAPDH (1:2,000; Beyotime, Shanghai, China) and anti-HNRNPA2B1 (1:1,000, Proteintech Group, Rosemont, IL, USA). Phenylmethylsulfonyl fluoride used in Western blot assay was from Selleck (Houston, TX, USA).

Guo H., Wang B., Xu K., Nie L., Fu Y., Wang Z., Wang Q., Wang S, & Zou X. (2020). m6A Reader HNRNPA2B1 Promotes Esophageal Cancer Progression via Up-Regulation of ACLY and ACC1. Frontiers in Oncology, 10, 553045.

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