Sk gt 4

Human cell lines (OE33, SK-GT-4) were purchased from European Collection of Cell Cultures. OE33, SK-GT-4 were grown in complete RPMI 1640 medium with 2 mM L-glutamine (Gibco) and supplemented with 1% (v/v) penicillin–streptomycin (50 U/ml penicillin 100 μg/ml streptomycin) and 10% (v/v) foetal bovine serum (Gibco) and maintained in a humidified chamber at 37 °C 5% CO₂. Cell lines were tested regularly to ensure mycoplasma negativity. The OE33 cell line was established from a poorly differentiated stage IIA adenocarcinoma of the lower oesophagus (Barrett’s metaplasia) of a 73-year old female patient. SK-GT-4 cell line originated from a well differentiated oesophageal adenocarcinoma arising in Barrett’s epithelium from an 89-year old Caucasian male.

OE33 and SK-GT-4 cell lines were purchased from the European Collection of Cell Cultures (ECACC, UK). All cell lines were cultured in RPMI 1640 with 10% FCS and 1mM L-Glutamine and Penicillin/Streptomycin at 37°C and 5% CO₂. The SK-GT-4 pcmv and miR-145 cell lines were established as previously described [15 (link)].

Five EGJ adenocarcinoma cell lines, OACM5.1C, OE19, OE33, SK-GT-4 and FLO-1, were purchased from the European Collection of Cell Cultures (ECACC; Salisbury, UK). FLO-1 was cultured in DMEM (Sigma-Aldrich, St Louis, MO, USA) containing 10% fetal bovine serum (FBS) in a 5% CO₂ air-humidified atmosphere at 37°C. The other cell lines (OACM5.1C, OE19, OE33 and SK-GT-4) were cultured in RPMI-1640 (Life Technologies, Carlsbad, CA, USA) under the same conditions, according to the manufacturer's recommendations.