Mice expressing GFP in their microglia were obtained by crossing G2019S-LRRK2 heterozygous mice (GS/-) with Cx3cr1–GFP (GFP/GFP) mice. For open-skull craniotomy surgery, mice were anaesthetized with Zoletil 50 (Virbac, intramuscular injection, 30 μl), fixed on the stereotactic heating plate (Live Cell Instruments, Seoul, Korea). After removing the scalp and the periosteum, the region (1 mm from bregma, 1 mm from sagittal suture; diameter, 3 mm) of the skull was drilled with a microdrill. Then, the bone was removed elaborately. Three-millimetre-round coverslip was attached to the region with Loctite 454 (Loctite, Rock Hill, CT, USA). Last, emerged portions of the skull were covered with dental acryl. Multiphoton imaging was performed using a LSM 7 MP two-photon laser-scanning microscope (Carl Zeiss Microscopy GmbH, Oberkochen, Germany)71 (link). Briefly, laser (920-nm wavelength, 30-mW intensity) was transiently applied to the brain (135-μm depth from the surface). The areas uncovered by microglia were measured, and plotted time-dependent changes in the area. Image Analysis Software (Perkin-Elmer, Waltham, MA, USA) was used to process the images and track the movements of microglia.
Image analysis software
Manufactured by PerkinElmer
Sourced in United States
The Image Analysis Software is a digital imaging platform designed to capture, analyze, and interpret visual data. It provides a suite of tools for image processing, measurement, and quantification to support scientific and research applications.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using image analysis software
1
Visualizing Microglia Dynamics in Parkinsonian Mice
2
Evaluating DNCaNPs' Cell Killing Ability
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To evaluate the cell killing ability of such DNCaNPs, CT26 cells preseeded in the 96-well plate at a density of 1 × 104 cells/well were incubated with CaNPs, aNLG919, free DOX, DCaNPs, and DNCaNPs at a series concentration of DOX and corresponding concentrations of aNLG919. 48 h later, the relative cell viabilities after various treatments were determined by the standard methyl thiazolyl tetrazolium (MTT) assay.
Additionally, when their diameter approached to ~ 400 μm, these CT26 MCSs (n = 5) were incubated with DNCaNPs, DCaNPS, or DNNPs (DOX = 10 μM) at pH 7.4 and 6.5 for 4 days. The optical images of these treated CT26 MCSs were recorded every day, and their diameters were measured using the ImageAnalysis software (PerkinElmer).
Additionally, when their diameter approached to ~ 400 μm, these CT26 MCSs (n = 5) were incubated with DNCaNPs, DCaNPS, or DNNPs (DOX = 10 μM) at pH 7.4 and 6.5 for 4 days. The optical images of these treated CT26 MCSs were recorded every day, and their diameters were measured using the ImageAnalysis software (PerkinElmer).
3
Monitoring DD-regulated Luciferase In Vivo
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Example 94
DD luciferase constructs can be utilized as an optical reporter to asses if the ligand and/or the cells expressing the DD constructs reach the targeted tissue. It may also be utilized to study pharmacokinetic and pharmacodynamic (PK/PD) relationships in the context of DD. PK/PD depends on (i) the PK of the stabilizing ligand (ii) behavior of the DD in a specific cell type (iii) cargo protein behavior; some of which may be studied by utilizing the DD regulated luciferase constructs. DD luciferase constructs are expressed or co-expressed in cells of interest such as primary T cells or cell lines e.g. HCT-116, SKOV-3 cells and injected into immune compromised mice e.g. via tail vein, intra peritoneal, or subcutaneous injections. Mice are treated with the corresponding ligand or vehicle control. 8-24 hours following ligand injection, mice are injected with D-Luciferin when the payload is firefly luciferase and Coelentrazin when the payload is Renilla luciferase. Animals are anesthetized and imaged using the Bioluminescence imager (PerkinElmer, Mass.). The luciferase output of ligand injected mice is compared to control mice and the signal is quantitated using image analysis software (PerkinElmer, Mass.). Luciferase signal is expected to be much higher than background in mice treated with ligand compared to mice treated with vehicle control.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!