Tc 100 insect medium

Bombyx mori 5 (Bm5) cells were cultured at 27 °C and pH 6.4 in TC-100 insect medium (WelGENE, Gyeongsan, GB, Korea) that was supplemented with 10% fetal bovine serum [18 (link)]. The wild-type (K1) and recombinant B. mori nucleopolyhedroviruses (BmNPVs) used in this study were propagated in Bm5 cells. The classical swine fever virus (CSFV LOM strain, GenBank accession number EU789580) and porcine parvovirus (PPV VRI-1 strain, GenBank accession number AY390557) provided from Optipharm Inc. were used in this study. Routine cell culture maintenance and virus production procedures were performed according to the published procedures [19 (link)].

Spodoptera frugiperda 9 (Sf9) and Bombyx mori 5 (Bm5) cells were maintained at 27°C in SF900 II serum-free medium (Gibco, USA) and TC-100 insect medium (WelGENE, Korea) supplemented with 10% fetal bovine serum. Wild-type Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) C6 and Bombyx mori NPV (BmNPV) K1 strains were used in this study (S1 Fig). Recombinant vApEGFP and vBpEGFP expressing the enhanced green fluorescent protein (EGFP) gene under the control of the polyhedrin promoter were used as controls (S1 Fig). Routine cell culture maintenance and virus production procedures were performed according to published procedures [4 ]