Fully differentiated ADMSCs-derived adipocytes were transfected using the Viromer GREEN transfection reagent (Lipocalyx, Halle, Germany) according to the manufacturer’s instruction. Briefly, siRNA targeting AS160/TBC1D4 at a concentration of 25 nM (Silencer Select Pre-designed siRNAs for AS160/TBC1D4—ID: s19140 and ID: s19142) and non-targeting control siRNA (Silencer Select Negative Control #1 siRNA) were used. The standard complexation protocol was employed using Viromer GREEN (Lipocalyx) according to the manufacturer's instruction.
Viromer green transfection reagent
Manufactured by BioNTech
Sourced in Germany
Viromer GREEN is a lipid-based transfection reagent designed for efficient delivery of nucleic acids, including plasmid DNA, mRNA, and siRNA, into a variety of cell types. It facilitates the uptake of these molecules into the target cells, enabling their study or therapeutic applications. The core function of Viromer GREEN is to enhance the transfection efficiency, allowing researchers to effectively introduce genetic material into their cell cultures.
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Lab products found in correlation
2 protocols using viromer green transfection reagent
1
Adipocyte Silencing of AS160/TBC1D4
2
Mincle Gene Knockdown in THP-1 Cells
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Gene knockdown was carried out using Trilencer-27 siRNA pool against Mincle or a scrambled siRNA control (OriGene Technologies, MD). Briefly, prior to transfection differentiated THP-1 cells (2×105) seeded in 24-well plates were incubated in serum free RPMI 1640 medium for 24 h. The cells were then transfected with Mincle or control siRNA using the Viromer Green transfection reagent as per the manufacturers recommendation (Lipocalyx, Halle, Germany). Five hours post transfection, the medium was replaced with fresh complete RPMI, and cells were maintained for another 72h.
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