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Cyanine 5.5 cy5.5

Manufactured by Merck Group
Sourced in United States

Cyanine 5.5 (Cy5.5) is a fluorescent dye used in various laboratory applications. It has an excitation maximum at 675 nm and an emission maximum at 694 nm, making it suitable for detection and analysis in the near-infrared range. Cyanine 5.5 is commonly used for labeling biomolecules, such as proteins and nucleic acids, in techniques like flow cytometry, fluorescence microscopy, and microarray analysis.

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2 protocols using cyanine 5.5 cy5.5

1

Establishing Hyaluronic Acid-Boronic Acid Conjugates

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CUR (> 98.0% purity) was obtained from Solarbio (China). 4-Hydroxyphenylboronic acid pinacol ester (PBAP), N,N’-carbonyldiimidazole (CDI), and sodium hyaluronate (HA) were acquired from Macklin (China). The reverse transcription kit was purchased from Accurate Biology (Hunan, China). The RNAeasy™ Animal RNA Extraction Kit and Reactive Oxygen Species Assay Kit were provided by Beyotime (China). The SYBR Prime qPCR Set and Cell Counting Kit-8 (CCK-8) were procured from Bioground (China). Hematoxylin and eosin (H&E) staining kits and tartrate-resistant acid phosphatase staining kits (TRAP Stain Kit) were purchased from Solarbio (China). Cyanine 5.5 (Cy5.5) and lipopolysaccharide (LPS) were sourced from Sigma-Aldrich (USA). Live/Dead BacLight Bacterial Viability Kits were provided by Bestbio (China). All other reagents used were of analytical grade.
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2

Synthesis and Characterization of Fullerene Nanomaterials

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Solid C60 and Gd@C82 (99% purity) were purchased from Xiamen Funano Co. Ltd. (Xiamen, China). Anhydrous ethanol, methanol, NaOH, H2O2, HNO3, NaH2PO4, and Na2HPO4 were purchased from Beijing Chemical Works (Beijing, China). NaCl, β-alanine, OA, glutaraldehyde, osmium tetroxide, dithiothreitol, iodoacetamide, pentobarbital sodium, urea, acetonitrile, formic acid, dithiothreitol, iodoacetamide, tetraethylammonium bromide (TEAB), Cyanine5.5 (Cy5.5), and 5, 5-dimethyl-1-pyrroline N-oxide (DMPO) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Nile Red, Hoechst 33342, hematoxylin, eosin, oil red O, and optimal cutting temperature (OCT) compound were purchased from Solarbio (Beijing, China). All reagents and solvents were obtained commercially and used without further purification.
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