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Orca fusion cmos camera

Manufactured by Hamamatsu Photonics
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The ORCA Fusion CMOS camera is a high-performance imaging device designed for scientific and industrial applications. It features a CMOS image sensor that provides fast readout speeds, low noise, and high quantum efficiency. The camera is capable of capturing images and video at high resolutions and frame rates, making it suitable for a variety of imaging tasks.

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Lab products found in correlation

Eclipse ti2 e inverted, by Nikon (1 mentions) Nis elements ar software, by Nikon (1 mentions) Csu w1, by Hamamatsu Photonics (1 mentions) 35 mm glass bottom petri dish, by MatTek (1 mentions) Dmi8 inverted microscope, by Leica camera (1 mentions) Ti2 microscope, by Nikon (1 mentions) Gelzan, by Merck Group (1 mentions) Spectrax illuminator, by Lumencor (1 mentions)

5 protocols using orca fusion cmos camera

1

Fluorescence Microscopy of Transwell Samples

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Transwell slides were imaged on a motorized Eclipse Ti2-E inverted microscope (Nikon Instruments) with a SOLA LED engine (Lumencor) and an ORCA Fusion CMOS camera (Hamamatsu). Semrock filter sets were used for individual DAPI, GFP, TRITC, and Cy5 channels. Multichannel images were captured as Z-stacks using NIS-Elements AR software (Nikon Instruments, v. 5.30) with 40x Plan Apochromat Lambda objectives (Nikon Instruments) for a final resolution of 0.32 μm/pixel and 0.16 μm/pixel, respectively. The Z-stacks were compressed into 2D images using the Extended Depth of Focus (EDF) module in NIS-Elements.
Kanke K.L., Rayner R.E., Abel E., Venugopalan A., Suu M., Stack J.T., Nouri R., Guo G., Vetter T.A., Cormet-Boyaka E., Hester M.E, & Vaidyanathan S. (2024). Single-Stranded DNA with Internal Base Modifications Mediates Highly Efficient Gene Insertion in Primary Cells. bioRxiv.
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2

Spinning Disk Confocal Microscopy for Super-Resolution Imaging

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Sixteen-bit fluorescence images were acquired using an Olympus IXplore spinning disk confocal microscope (equipped with the Yokogawa CSU-W1 with 50 µm pinhole disk and a Hamamatsu ORCA Fusion CMOS camera). A 60× oil immersion objective (NA 1.42) in combination with a 3.2× magnification lens (equalling 192× total magnification) was used for super-resolution imaging of fixed cells and z-stacks with a 0.24 µm slice interval were acquired. These z-stacks were then processed using the Olympus 3D deconvolution software (cellSens Dimension 3.1) (constrained iterative deconvolution, using automatic background removal and noise reduction, filter using advanced maximum likelihood algorithm and five iterations). Finally, ‘maximum-z’ projection images of the deconvoluted z-stacks were generated. Olympus 3D deconvolution software (cellSens Dimension 3.1) was used for analysis. Nuclear foci were counted manually and at least 50 cells per condition were imaged in each experiment. Quantification of the foci was performed manually based on maximum intensity projections.
Ferreira da Silva J., Oliveira G.P., Arasa-Verge E.A., Kagiou C., Moretton A., Timelthaler G., Jiricny J, & Loizou J.I. (2022). Prime editing efficiency and fidelity are enhanced in the absence of mismatch repair. Nature Communications, 13, 760.
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3

Visualizing Pupal Fluid Secretion

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For imaging, 13- to 17-day-old melanized pupae were placed in the microwell of a 35 mm glass bottom Petri dish (MatTek) and covered with a coverslip. Abiotic conditions (100% humidity, 25 °C) were maintained throughout the recording using an environmental stage chamber (Okolab). Bright-field images of pupal fluid droplets secreted from the abdominal tip were acquired with a DMi8 inverted microscope (Leica), an Orca fusion CMOS camera (Hamamatsu) and the VisiTech InstantSIM (iSIM) (VisiTech International) real-time superresolution system with a 20×/0.75 water objective at a resolution of 3.08 pixels per µm. Imaging was performed at different depths. The z-plane depths were selected to optimize visualization of secretion droplets, the rectal invagination, and the genital opening. Images were acquired using VisiView acquisition software version 4.5.0.13 (VisiTech International) and image processing was performed using FIJI/ImageJ version 1.52p32 (link).
Snir O., Alwaseem H., Heissel S., Sharma A., Valdés-Rodríguez S., Carroll T.S., Jiang C.S., Razzauti J, & Kronauer D.J. (2022). The pupal moulting fluid has evolved social functions in ants. Nature, 612(7940), 488-494.
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4

Characterizing 3D Printed Microfluidic Valve Performance

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After 3D printing valve-integrated microfluidic devices, the performance of the valves was tested by first closing valves controlling fluid flow to the separation channel (Figure S1a) while leaving valves connected to the injection channel open. Next, fluorescent dye was pulled with vacuum into the injection intersection. Injection valve leakage testing was performed using 532 nm laser excitation with a Hamamatsu ORCA-Fusion CMOS camera (Bridgewater, NJ) to monitor flow of fluorescent dye into the separation channel. In an additional experiment, both valves 2 were closed, vacuum was turned on, and the injection intersection was observed for flow of fluorescent dye. Valve tests were performed with pressures between 25–45 psi. To further investigate the integrity of the valves, a point detection experiment was performed, wherein fluorescent dye was flowed into the injection channel (valves 2 open), and fluorescence was recorded in the separation channel (Figure S1a) using a Hamamatsu photomultiplier tube (PMT, Bridgewater, NJ).
Esene J.E., Boaks M., Bickham A.V., Nordin G.P, & Woolley A.T. (2022). 3D Printed Microfluidic Device for Automated, Pressure-driven, Valve-injected Microchip Electrophoresis of Preterm Birth Biomarkers. Mikrochimica acta, 189(5), 204.
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5

Fluorescent Bacterial Imaging Protocol

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Fluo-castalagin–labeled bacteria were loaded onto a slide with 0.2% Gelzan (Sigma) pads. Epifluorescence microscopy imaging of fluo-castalagin–labeled bacteria was performed using a Nikon Ti2 microscope equipped with a 60× CFI Plan Apochromat phase contrast objective with a numerical aperture of 1.40. Fluorescein imaging used a SpectraX illuminator (Lumencor) with an FITC filter set (470/24 nm excitation, 525/50 nm emission). Images were captured with a Hamamatsu Orca Fusion CMOS camera. Images were processed in FIJI as follows: images were cropped, the background from the fluorescence channel was subtracted, and an identical lookup table was applied to the fluorescence channels.
Messaoudene M., Pidgeon R., Richard C., Ponce M., Diop K., Benlaifaoui M., Nolin-Lapalme A., Cauchois F., Malo J., Belkaid W., Isnard S., Fradet Y., Dridi L., Velin D., Oster P., Raoult D., Ghiringhelli F., Boidot R., Chevrier S., Kysela D.T., Brun Y.V., Falcone E.L., Pilon G., Oñate F.P., Gitton-Quent O., Le Chatelier E., Durand S., Kroemer G., Elkrief A., Marette A., Castagner B, & Routy B. (2022). A Natural Polyphenol Exerts Antitumor Activity and Circumvents Anti–PD-1 Resistance through Effects on the Gut Microbiota. Cancer Discovery, 12(4), 1070-1087.
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