Hrp conjugated secondary antibodies 7074

Oxaliplatin and alantolactone were obtained from Aladdin (Shanghai, China). N-Acetyl-L-Cysteine (NAC) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Antibodies against primary antibodies including p-p38 (sc-9211), p38 (sc-9212), p-JNK (sc-4668), JNK (sc-9252) and HRP-conjugated secondary antibodies (7074) were obtained from Cell Signaling Technology (Danvers, MA). Ki-67 (ab15580) was obtained from Abcam (Cambridge, England). Fluorescein isothiocyanate (FITC) Annexin V Apoptosis Detection Kit I and Propidium Iodide (PI) were purchased from BD Pharmingen (Franklin Lakes, NJ). Reactive oxygen species probe 2′, 7′- dichlorodihydrofluorescin diacetate (DCFH-DA) was purchased from Thermo Fisher (Carlsbad, CA, USA). 3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyl-2 tetrazolium bromide (MTT) was obtained from Beyotime Biotech (Nantong, China).

Regorafenib and cisplatin were obtained from MedChemExpress (USA). N-Acety-l-Cysteine (NAC) were purchased from Sigma-Aldrich (St. Louis, MO, USA). DCFH-DA ROS detection kit were obtained from Beyotime (Shanghai, China). Antibodies of eukaryotic initiation factor-2α (eIF2α, 9722S), phosphorylated (p)-eIF2α (3398S), caspase-3 (9662S), cleaved caspase-3 (9664S), activating transcription factor 4 (ATF4, 11815S), p-JNK (4668S), JNK (9252T), p-p38 (9211S), p38 (9212S), GAPDH (5174S) and HRP-conjugated secondary antibodies (7074) were purchased from Cell Signaling Technology (Danvers, USA). Antibodies of NOX5 (25350-1-AP) and Bcl-2 (60178-1-Ig) were purchased from Proteintech (Wuhan, China).