Log In Sign up
The largest database of trusted experimental protocols

Pecy7 conjugated anti cd27

Manufactured by Beckman Coulter
Visit Supplier
Sourced in United States

PECy7-conjugated anti-CD27 is a fluorochrome-conjugated monoclonal antibody that binds to the CD27 surface antigen. CD27 is a member of the tumor necrosis factor receptor superfamily and is expressed on various immune cell types. The PECy7 fluorochrome allows for the detection and analysis of CD27-positive cells using flow cytometry.

Automatically generated - may contain errors

Lab products found in correlation

Fetal calf serum (fcs), by Merck Group (1 mentions) Sodium azide nan3, by Merck Group (1 mentions) 7 aminoactinomycin d 7 aad, by Merck Group (1 mentions) Cxp analysis, by Beckman Coulter (1 mentions) Navios flow cytometer, by Beckman Coulter (1 mentions)

2 protocols using pecy7 conjugated anti cd27

1

Multicolor Flow Cytometry Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols
Staining protocol was performed as previously described [7 ]. Briefly, ex vivo or in vitro assay’s cells were stained for surface markers with a panel of monoclonal antibodies, as follows: FITC-conjugated anti-CD3; APC-conjugated anti-CD8; PECy7-conjugated anti-CD27; ECD-conjugated anti-CD45 (all Beckman Coulter, Miami, FL, USA) in PBS containing 0.1% sodium azide (NaN3; Sigma Aldrich) and 2% fetal calf serum (Sigma Aldrich) and incubated for 20 minutes on ice. Afterwards, these samples were incubated with 20 μg/mL of 7-aminoactinomycin D (7-AAD; Sigma Aldrich) for 30 minutes at 4°C, for apoptosis evaluation, as described in elsewhere. The samples were kept on 7-AAD solution, protected from light, until the flow cytometry acquisition.
Ferraz R., Cunha C.F., Gomes-Silva A., Schubach A.O., Pimentel M.I., Lyra M.R., Mendonça S.C., Valete-Rosalino C.M., Da-Cruz A.M, & Bertho Á.L. (2015). Apoptosis and frequency of total and effector CD8+ T lymphocytes from cutaneous leishmaniasis patients during antimonial therapy. BMC Infectious Diseases, 15, 74.
+ Open protocol
+ Expand
2

Phenotypic Analysis of Human PBMC Subsets

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Fresh PBMCs were stained with the following conjugated anti-human monoclonal antibodies (mAb): fluorescein isothiocyanate (FITC)–conjugated anti-CD21, energy-coupled dye–conjugated anti-CD19, allophyocyanin (APC)–conjugated anti-IgM, and phycoerythrin (PE)–Cy7–conjugated anti-CD27 (Beckman Coulter). PBMCs were also stained with PE-conjugated anti–FCRL-1, anti–FCRL-2, anti–FCRL-3, anti–FCRL-4, and anti–FCRL-5 mAb. We prepared these mAb as described previously (31 (link)–34 (link)). All of these FCRL-specific mAb were authorized as reference antibodies for FCRL proteins 1–5 (CD307a–e) in previous international human leukocyte differentiation antigens workshops (33 (link),35 (link)). We also used PE-conjugated anti–FCRL-4 mAb (clone 413D12) from BioLegend. PE-conjugated IgG1 (Beckman Coulter) was used as an isotype control. Flow cytometry was performed on a Navios flow cytometer (Beckman Coulter) and analyzed using CXP analysis (Beckman Coulter) and FlowJo software (Tree Star).
Terrier B., Nagata S., Ise T., Rosenzwajg M., Pastan I., Klatzmann D., Saadoun D, & Cacoub P. (2014). CD21−/low Marginal Zone B Cells Highly Express Fc Receptor–like 5 Protein and Are Killed by Anti–Fc Receptor–like 5 Immunotoxins in Hepatitis C Virus–Associated Mixed Cryoglobulinemia Vasculitis. Arthritis & rheumatology (Hoboken, N.J.), 66(2), 433-443.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!