Mouse anti gfp

Manufactured by Developmental Studies Hybridoma Bank

Sourced in United States

Mouse anti-GFP is a monoclonal antibody specifically designed to detect and bind to the green fluorescent protein (GFP) epitope. This antibody can be used in various biological applications that involve the detection and visualization of GFP-tagged proteins.

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Lab products found in correlation

Goat anti mouse igg alexa 568, by Thermo Fisher Scientific (1 mentions) Goat anti rabbit igg alexa 568, by Thermo Fisher Scientific (1 mentions) Mouse anti gfp, by Merck Group (1 mentions) Formaldehyde, by Merck Group (1 mentions) Rabbit anti myc, by Merck Group (1 mentions) Mouse anti β tubulin, by Merck Group (1 mentions) Goat anti mouse igg alexa 488, by Thermo Fisher Scientific (1 mentions) Goat anti rabbit igg alexa 647, by Thermo Fisher Scientific (1 mentions) Rabbit anti phospho smad1 5, by Cell Signaling Technology (1 mentions) Gfp booster, by Proteintech (1 mentions) Mouse anti dlg1, by Developmental Studies Hybridoma Bank (1 mentions) Mouse anti rfp, by Proteintech (1 mentions) Rabbit anti apkc, by Santa Cruz Biotechnology (1 mentions) Rabbit anti rab5, by Abcam (1 mentions) Rabbit anti rfp, by Abcam (1 mentions) Rat anti de cadherin, by Developmental Studies Hybridoma Bank (1 mentions) Alexa 633, by Thermo Fisher Scientific (1 mentions) Alexa 555, by Thermo Fisher Scientific (1 mentions) Triton x 100, by Merck Group (1 mentions) Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Anti-Gfp (2 citations)

4 protocols using mouse anti gfp

Immunohistochemical Analysis of Pupal Wings

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Pupal wings were fixed in 3.7% formaldehyde (Sigma-Aldrich) at 4°C overnight. Wing imaginal discs were fixed in 3.7% formaldehyde at room temperature (RT) for 20 minutes. All immunostaining and in situ hybridizations were performed as described previously [7 (link),9 (link)]. The primary antibodies used are as follows: mouse anti-DLG1, rat anti-DE-Cadherin and mouse anti-GFP (for immunohistochemistry; all at 1:50) were obtained from Developmental Studies Hybridoma Bank, rabbit anti-phospho-SMAD1/5 (1: 200 for IF, 1:2000 for Western blotting) from Cell Signaling Technology (CST), rabbit anti-Rab5 (1:600) and rabbit anti-RFP (1:5000 for Western blotting) from Abcam, mouse anti-RFP (1:5000 for Western blotting) from Chromotek, mouse anti-GFP (1: 5000 for Western blotting) from Millipore, mouse anti-β-tubulin (1:5000) from Sigma-Aldrich, rabbit anti-MYC (1:500), goat anti-Scrib (1:100), rabbit anti-aPKC (1:100) and mouse anti-LGL (1:200) from Santa Cruz Biotechnology, and rabbit anti-Scrib (1:2000) from C. Doe. Secondary antibodies were as follows: goat anti-mouse IgG Alexa 488, goat anti-mouse IgG Alexa 568, goat anti-mouse IgG Alexa 647, goat anti-rabbit IgG Alexa 568, goat anti-rabbit IgG Alexa 647, goat anti-rat IgG Alexa 488 and goat anti-mouse IgG Cy5, all from Molecular Probes (1:200). GFP-booster (1:200, ChromoTek) was used to enhance the YFP signal in Fig 3F and S2C Fig.

Gui J., Huang Y, & Shimmi O. (2016). Scribbled Optimizes BMP Signaling through Its Receptor Internalization to the Rab5 Endosome and Promote Robust Epithelial Morphogenesis. PLoS Genetics, 12(11), e1006424.

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Visualization of Histone Acetylation and Sex Chromosomes

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Hydrated, fixed embryos were incubated in the following primary antibodies overnight at 4°C: mouse anti-GFP (1:50, clone number 4C9, Developmental Studies Hybridoma Bank); goat anti-MOF (1:100, clone number dn-17, Santa Cruz Biotech); rabbit anti-acetyl-Histone H4 Lysine 16 (1:100, catalog number 07-329, Millipore). Following incubation with primary antibodies, embryos were washed 3×15min in 1xPBT before secondary antibody staining. Anti-mouse, anti-rabbit, and anti-goat secondary antibodies were conjugated with either Alexa555 or Alexa633 (Molecular Probes-Invitrogen) and used at a dilution of 1:300. Secondary staining was conducted out of light for 1hr at room temperature and washed three times subsequently.
Fluorescence in situ hybridization was conducted with an oligonucleotide probe specific for a satellite DNA sequence unique to the Y chromosome [35 (link)]. The probe consisted of the sequence 5’-AAT ACA ATA CAA TAC AAT ACA ATA CAA TAC-‘3 and was synthesized commercially (IDT, Inc.) with Cy2 conjugated at the 5’ end. Whole mount embryo hybridizations were conducted as described [35 (link)], except that embryos used for this purpose were stained first with antibodies, re-fixed in 4% paraformaldehyde for 45 minutes, and washed 3X in 2xSSCT buffer before probe hybridization.

Cheng B., Kuppanda N., Aldrich J.C., Akbari O.S, & Ferree P.M. (2016). Male-killing Spiroplasma Alters Behavior of the Dosage Compensation Complex during Drosophila melanogaster embryogenesis. Current biology : CB, 26(10), 1339-1345.

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Immunostaining of Neuronal Cultures

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Neurons (10 DIV) were fixed in 4% paraformaldehyde in PBS for 20 min or, in the case of pan-Nav1 stainings, for 2 min in 2% paraformaldehyde in PBS followed by 10 min in 100% methanol at −20°C. Blocking of unspecific binding was performed for 30 min with 0.2% fish skin gelatin (Sigma-Aldrich) in PBS for surface-staining or in 0.1% Triton X-100 (Sigma) in PBS (PBST) for total-staining for 30 min at room temperature (RT). Next, neurons were incubated with primary antibodies diluted in 0.2% fish skin gelatin in PBS or PBST for 1 h at RT. Lastly the neurons were incubated with secondary antibodies diluted in 0.2% fish skin gelatin in PBS or PBST for 45 min at RT. Primary antibodies used: mouse anti-CD4 (1:25–1:50 dilution; clone 18–46; Santa Cruz Biotechnology), mouse anti-CD4 (1:50 dilution, MT310, Santa Cruz Biotechnology), rabbit anti-MAP2 (1:100 dilution, H-300, Santa Cruz Biotechnology), mouse anti-pan-Nav1 (1:100 dilution, clone N419/40, RRID:AB_2491098, Neuromab), mouse anti-ankG (1:5, clone N106/65, RRID: AB_10673449, Neuromab), mouse anti-GFP (1:5, clone 4C9, Developmental studies Hybridoma Bank). Primary antibodies were detected using AlexaFluor^®-conjugated secondary antibodies (Thermo Fischer Scientific). Coverslips were mounted on microscope slides using ProLong Gold or Diamond Antifade Reagent (Thermo Fischer Scientific).

Hefting L.L., D’Este E., Arvedsen E., Benned-Jensen T, & Rasmussen H.B. (2020). Multiple Domains in the Kv7.3 C-Terminus Can Regulate Localization to the Axon Initial Segment. Frontiers in Cellular Neuroscience, 14, 10.

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Dissection and Immunolabeling of Larval Nervous System

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Larvae were dissected as filets preparations to preserve the fine structure of the whole nervous system and fixed for 20 min in 4% paraformaldehyde (PFA) or for 3 min in Bouins’s solution (Sigma-Aldrich) followed by standard immuno-labeling procedures. The following antibodies were used: rabbit anti-GFP (1:1000, LifeTechnologies), mouse anti-repo (1D48D12, 1:100), mouse anti-GFP (12A6,1:500), mouse anti-Brp (nc82,1:250), mouse anti-Futsch (22C10,1:250) all from Developmental Studies Hybridoma Bank; rabbit anti-p24-1 (kind gift from G. Carnery; Texas A&M University, US), mouse anti-HA (F7, 1:100, Santa Cruz) and rabbit anti-HA (#9110,1:250, Abcam), and anti-HRP-Alexa637 (1:300, Jackson Immuno Research). Secondary antibodies with Alexa 488, Alexa555 or Alexa647 conjugate (LifeTechnologies) were used in a dilution of 1:1000. Samples were mounted in VectaShield anti fade reagent (H-1000, Vector Laboratories). Confocal images were taken with Zeiss LSM 510 Meta microscope.

Mukherjee C., Kling T., Russo B., Miebach K., Kess E., Schifferer M., Pedro L.D., Weikert U., Fard M.K., Kannaiyan N., Rossner M., Aicher M.L., Goebbels S., Nave K.A., Krämer-Albers E.M., Schneider A, & Simons M. (2020). Oligodendrocytes Provide Antioxidant Defense Function for Neurons by Secreting Ferritin Heavy Chain. Cell metabolism, 32(2), 259-272.e10.

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