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Desalted dna oligonucleotides

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Desalted DNA oligonucleotides are single-stranded synthetic DNA molecules designed for various applications in molecular biology and biotechnology. They are produced by removing excess salts and other impurities from the DNA synthesis process, resulting in a purified product.

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Lab products found in correlation

T4 dna ligase, by New England Biolabs (1 mentions) Phusion and taq dna polymerases, by New England Biolabs (1 mentions) T4 polynucleotide kinase, by New England Biolabs (1 mentions) Trypsin, by Promega (1 mentions)

Spelling variants of this product

Oligonucleotides (424 citations) DNA oligonucleotides (166 citations) Desalted oligonucleotides (6 citations) Standard desalted DNA oligonucleotides (2 citations)

2 protocols using desalted dna oligonucleotides

1

Biochemicals and Molecular Biology Reagents

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Biochemicals were purchased from Sigma-Aldrich (St. Louis, MO). Other organic and inorganic chemicals were analytical-grade from Fisher Scientific (Atlanta, GA) and Bio-Rad (Hercules, CA). Desalted DNA oligonucleotides were from Integrated DNA Technologies (Coralville, IN). Phusion and Taq DNA polymerases, restriction enzymes, T4 polynucleotide kinase and T4 DNA ligase were from New England Biolabs (Ipswich, MA). Hi-Lo DNA standards were from Minnesota Molecular, Inc. (Minneapolis, MN). Trypsin was sequencing grade from Promega (Madison, WI).
Chavarria N.E., Hwang S., Cao S., Fu X., Holman M., Elbanna D., Rodriguez S., Arrington D., Englert M., Uthandi S., Söll D, & Maupin-Furlow J.A. (2014). Archaeal Tuc1/Ncs6 Homolog Required for Wobble Uridine tRNA Thiolation Is Associated with Ubiquitin-Proteasome, Translation, and RNA Processing System Homologs. PLoS ONE, 9(6), e99104.
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2

Oligonucleotide Design and Purification

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Desalted DNA oligonucleotides were purchased (Integrated DNA Technologies) at 1 μmol synthesis scale and dissolved in one of the buffers described below to give 10 mM solutions. The modifications ordered are outlined in Figure 1 and a table listing all oligonucleotides is included in the Supplementary Material. Chemicals were purchased from Sigma or as indicated. A zinc determination kit (Abcam) was used to select only zinc-containing A3A–E72A in chromatographic separations.
Harjes S., Jameson G.B., Filichev V.V., Edwards P.J, & Harjes E. (2017). NMR-based method of small changes reveals how DNA mutator APOBEC3A interacts with its single-stranded DNA substrate. Nucleic Acids Research, 45(9), 5602-5613.
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