γ catenin

Manufactured by Santa Cruz Biotechnology

Sourced in United States

γ-CATENIN is a cytoplasmic protein that plays a role in cell-cell adhesion and the Wnt signaling pathway. It is a key component of the cadherin-catenin complex, which is involved in the regulation of cell-cell adhesion and cell signaling.

Automatically generated - may contain errors

Lab products found in correlation

Ecl detection kit, by Cytiva (1 mentions) Fibronectin, by BD (1 mentions) E cadherin, by BD (1 mentions) Vimentin, by BD (1 mentions) Myd88, by Santa Cruz Biotechnology (1 mentions) Phospho β catenin, by Cell Signaling Technology (1 mentions) Tfiib, by Santa Cruz Biotechnology (1 mentions) C myc, by BD (1 mentions) Notch1, by Abcam (1 mentions) Nanog, by Abcam (1 mentions) β catenin, by BD (1 mentions) Twist, by Santa Cruz Biotechnology (1 mentions) Aldh1, by Abcam (1 mentions) Snail1, by Santa Cruz Biotechnology (1 mentions) Foxm1, by Santa Cruz Biotechnology (1 mentions) N cadherin, by Cell Signaling Technology (1 mentions) Vascular endothelial growth factor (vegf), by R&D Systems (1 mentions) β catenin, by Santa Cruz Biotechnology (1 mentions) Endothelial cell basal medium ebm, by Lonza (1 mentions) Ve cadherin, by Santa Cruz Biotechnology (1 mentions)

2 protocols using γ catenin

Western Blot Analysis of EMT and Stemness Markers

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Cell lysates were harvested in ice-cold modified radioimmune precipitation assay buffer containing a protease inhibitor cocktail^TM (Roche). Lysates normalized for amount protein were separated on 10% SDS-polyacrylamide gels and electrophoretically transferred to nitrocellulose. The blots were incubated with primary antibodies to E-CADHERIN, VIMENTIN, FIBRONECTIN, β-CATENIN (BD), c-MYC, FOXM1, TFIIB, MYD88, γ-CATENIN, SNAIL1, SLUG, TWIST, hCTR1 (Santa Cruz), BMI1, CD44, NANOG, ALDH-1, OCT-4, NOTCH-1, SFRP5 (Abcam), ZO-1 (Invitrogen), phospho-β-CATENIN, N-CADHERIN (Cell Signaling), or SOX-2 (Sigma). Immunocomplexes were detected with horseradish peroxidase-conjugated IgG and visualized via enhanced chemiluminescence (ECL detection kit, Amersham).

Chiu W.T., Huang Y.F., Tsai H.Y., Chen C.C., Chang C.H., Huang S.C., Hsu K.F, & Chou C.Y. (2014). FOXM1 confers to epithelial-mesenchymal transition, stemness and chemoresistance in epithelial ovarian carcinoma cells. Oncotarget, 6(4), 2349-2365.

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Investigating VEGF-Induced Signaling Pathways

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VEGF was purchased from R&D Systems (Minneapolis, MN). PMA, histamine, and antibody against Flag tag (Cat. No. F-3165) were purchased from Sigma (St. Louis, MO). Antibodies against TR3/Nur77, cyclin D1, VE-cadherin, β-catenin, γ-catenin, p120, and claudin 5 were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Endothelial cell basal medium (EBM) and EGM-MV BulletKit were obtained from Lonza (Allendale, NJ). Vitrogen 100 was purchased from Collagen Biomaterials (Palo Alto, CA, USA). BAPTA/AM, cyclosporine-A, W-7, KN62, U-73122, GF, CID2011756, PD98059, SB203580, JNK inhibitor II, AG1024, IP3R inhibitor, 2-APB, and rottlerin are the products of Calbiochem (Billerica, MA, USA). Anti-phospho-IGF-1Rβ antibodies were obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA). Anti-IGF-1Rα antibody was obtained from EMD Millipore (Billerica, MA, USA).

Zhou L., Cui P., Zhao S., Ye T., Li Y., Peng J., Niu G., Zhao D, & Zeng H. (2015). Differential function and regulation of orphan nuclear receptor TR3 isoforms in endothelial cells. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 37(3), 3307-3320.

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