Alexa fluor 700 antihuman ki67 antibody

Manufactured by BioLegend

The Alexa Fluor 700 antihuman Ki67 antibody is a fluorescently labeled antibody that specifically binds to the Ki67 protein, which is a marker of cell proliferation. This antibody can be used in flow cytometry and other immunoassays to detect and quantify Ki67-expressing cells.

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Lab products found in correlation

Cd152 pe cy5, by BD (1 mentions) Fixable viability dye efluor 450, by BioLegend (1 mentions) Cd4 bv605, by BioLegend (1 mentions) Pd1 apc, by BioLegend (1 mentions) Ebioscience foxp3 transcription factor staining buffer set, by Thermo Fisher Scientific (1 mentions) Lung dissociation kit, by Miltenyi Biotec (1 mentions) Lsrfortessa flow cytometer, by BD (1 mentions)

Close spelling variants of this product

Alexa Fluor 700 (29 citations) Ki67 antibody (7 citations)

2 protocols using alexa fluor 700 antihuman ki67 antibody

Immune Phenotyping of PBMC Samples

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Cryopreserved peripheral blood mononuclear cell (PBMC) samples from baseline and post-treatment (cycles 1–12) were thawed and rested overnight at 37°C. Then cells were stained with a viability dye (eBioscience Fixable Viability Dye eFluor 450) and a master mix of antibodies for surface stains including CD4-BV605 (BioLegend Cat# 317438, RRID:AB_11218995), CD3-PE-Cyanine5.5 (Invitrogen Cat# 35-0036-42, RRID: AB_11220085), CD8a-PE-Cyanine7 (Invitrogen Cat# 25-0088-42, RRID: AB_1659702), PD1-APC (BioLegend Cat# 329908, RRID: AB_940475), CD152-PE-Cy5 (BD Biosciences Cat# 555854, RRID:AB_396177). Cells were next fixed and permeabilized with the eBioscience Foxp3/Transcription Factor Staining Buffer Set (ThermoFisher) and subsequently stained intracellularly with Alexa Fluor 700 antihuman Ki67 antibody (BioLegend Cat# 350530, RRID: AB_2564040). Stained cells were acquired on a BD Canto RUO and analyzed with FlowJo software (FlowJo, RRID:SCR_008520).

Liao J.B., Gwin W.R., Urban R.R., Hitchcock-Bernhardt K.M., Coveler A.L., Higgins D.M., Childs J.S., Shakalia H.N., Swensen R.E., Stanton S.E., Tinker A.V., Wahl T.A., Ancheta R.G., McGonigle K.F., Dai J.Y., Disis M.L, & Goff B.A. (2021). Pembrolizumab with low-dose carboplatin for recurrent platinum-resistant ovarian, fallopian tube, and primary peritoneal cancer: survival and immune correlates. Journal for Immunotherapy of Cancer, 9(9), e003122.

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Quantifying Luciferase-Positive Melanoma Cells

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Mouse lung tissues were dissociated into single cell suspensions using Lung Dissociation Kit (Miltenyi Biotec, #130-095-027) according to the manufacturer’s instructions. The cells were fixed and permeabilized with cold 70% ethanol, washed with PBS twice, and stained with fluorochrome-conjugated antibodies for 1 hr at RT in dark condition. Alexa Fluor 647 anti-firefly luciferase antibody was purchased from Abcam (Cambridge, MA), Alexa Fluor 700 anti-human Ki-67 antibody was purchased from Biolegend (San Diego, CA), and Alexa Fluor 488 anti-human p27 antibody was purchased from Santa Cruz (Dallas, TX). Each Ki-67 and p27 fluorescence signal was gated and analyzed the signal intensity from the first gating of melanoma cells with high fluorescence intensity of Alexa Fluor 647 luciferase signal. Flow cytometry was performed with BD LSR Fortessa™ Flow Cytometer (BD Biosciences) and data was analyzed with FlowJo (FlowJo Inc.).

Min D., Byun J., Lee E.J., Khan A.A., Liu C., Loudig O., Hu W., Zhao Y., Herlyn M., Tycko B., Cole P.A, & Ryu B. (2021). Epigenetic Silencing of BMP6 by the SIN3A-HDAC1/2 Repressor Complex Drives Melanoma Metastasis via FAM83G/PAWS1. Molecular cancer research : MCR, 20(2), 217-230.

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