Rabbit igg 7074p2

NaF (S6776) was purchased from Sigma Aldrich, UK. Lymphocyte separation medium (DKW33-R0100) were supplied by Dakewe Biotech Company, China. JC-1 mitochondrial membrane potential assay kit (C2006), ROS Assay kit (S0033), RIPA lysis buffer (P0013C), BCA Protein Assay Kit (P0012), Cell Counting Kit-8 (CCK-8) (C0038) and Hoechst 33258 (C1011) were obtained from Beyotime Biotechnology, China. RPMI 1640 (11875119) and fetal bovine serum (16000044) were supplied by Gibco, UK. The mouse Bc1-2 (3498T), Bcl-xl (2764T), Bax (14796S), Bak (12105T), caspase-9 (9508T), cleaved caspase-8 (8592T), cleaved caspase-7 (8438S), cleaved caspase-6 (9761T), cleaved caspase-3 (9664T) antibodies, rabbit IgG (7074P2) and mouse IgG (7076P2) were obtained from Cell Signaling Technology, UK. Fas (ab82419) and FasL (ab16104) were purchased from Abcam, UK. All other reagents used were analytical grade.

NaF (S6776) was purchased from Sigma Aldrich, UK. Lymphocyte separation medium (DKW33-R0100) were supplied by Dakewe Biotech Company, China. RIPA lysis buffer (P0013C), BCA Protein Assay Kit (P0012), Cell Counting Kit-8 (CCK-8) (C0038) and Annexin V-PE/7-AAD Apoptosis Detection Kit I (559763) were obtained from BD, USA. RPMI 1640 (11875119) and fetal bovine serum (16000044) were supplied by Gibco, UK. The mouse Bip (3177T), GRP94 (20292T), CHOP (2895P), ATF6 (65880S), p-JNK (4668T), p-eIF2a (3398P), ATF4 (11815S), cleaved caspase-12 (ab18766) antibodies, mouse IgG (7076P2) and rabbit IgG (7074P2) were obtained from Cell Signaling Technology, UK. p-PERK (sc-32577) were obtained from Santa Cruz Biotechnology, USA, p-IRE1 (ab48187) were obtained from Abcam, UK.