BG levels were determined by using the Fungitell® kit (Associates of Cape Cod, Inc., Cape Cod, MA) according to the manufacturer’s instructions. The BG concentrations in samples were calculated automatically by using a calibration curve established with standard solutions ranging from 31 to 500 pg/ml. A cut-off BG level of ≥80 pg/ml was considered as a positive test result as defined by the manufacturer. Assays were performed in duplicate. Samples with levels higher than 500 pg/ml were 10-fold diluted and reassayed. After dilution, samples with levels higher than 5,000 pg/ml were not diluted again and the value 5,000 pg/ml was considered for statistical analysis. BG levels below 31 pg/ml (lower quantification limit) were systematically set at 31.
Fungitell kit
Manufactured by Associates of Cape Cod
Sourced in United States
The Fungitell kit is a laboratory diagnostic product manufactured by Associates of Cape Cod. The kit is designed to detect the presence of (1->3)-β-D-Glucan, a component of the cell wall of various fungi. The Fungitell kit provides a quantitative measurement of (1->3)-β-D-Glucan levels in biological samples.
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Lab products found in correlation
8 protocols using fungitell kit
1
Fungitell® Assay for Beta-Glucan Quantification
2
Serum Biomarkers for Fungal Infections
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The BDG concentration was measured by using the Fungitell kit (Associates of Cape Cod Inc., Falmouth, MA, USA) according to the manufacturer's instructions. The recommended cutoff of 80 pg/ml was used to determine clinical relevance. Measurement of serum Mnn levels was performed by using the Platelia Candida Ag+ test (Bio-Rad, Marnes la Coquette, France) according to the manufacturer's instructions. The recommended cutoff of 62.5 pg/ml was used to determine clinical relevance.
For both tests, serum samples with positive results of >500 pg/ml were diluted and retested.
For both tests, serum samples with positive results of >500 pg/ml were diluted and retested.
3
Biomarkers of Inflammation and Intestinal Damage
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Biomarkers were measured on EDTA plasma or serum. Intestinal fatty acid binding protein (I-FABP) was measured using the DuoSet ELISA Kit; sCD14 and IL-6 were measured by ELISA using Quantikine kits; sCD163 and sTNFRII were measured using Luminex high-performance assays (all R&D systems, Minneapolis MN). Serum (1,3)-β-D-glucan was measured using the Fungitell® kit (Associates of Cape Cod, Inc., Falmouth, MA). Other inflammation biomarkers were measured using the Luminex platform (Bio-Plex Pro™ Human Inflammation Panel 1, Bio-Rad Laboratories, Inc., Hercules, CA).
4
Fungitell Kit for β-glucan Measurement
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β-glucan levels were determined for all subjects on several time points using the Fungitell kit (Associates of Cape Cod, Inc., Cape Cod, MA), according to the manufacturer's instructions [11] (link). Lower and upper detection limits were 31.25 and 500 pg/ml, respectively.
5
Fungal Biomarker Detection Protocol
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For the diagnosis of IC and IA, detection of mannan (Man) or galactomannan (GM) and (1,3)-β-d -glucan (BDG) was performed in the participating centers during routine patient screening or in the Lille Clinical Mycology Laboratory if they had not been tested previously.
BDG was measured using a Fungitell kit (Associates of Cape Cod Inc., Falmouth, MA, USA) following the manufacturer’s instructions. The recommended cutoff of 80 pg/ml was used to determine clinical relevance.
Measurement of serum Man and GM was performed using a Platelia Candida Ag Plus test and Platelia Aspergillus Ag test (Bio-Rad, Marnes la Coquette, France), respectively, according to the manufacturer's instructions. The recommended cutoffs of a concentration of 62.5 pg/ml and an index of ≥0.5, respectively, were used.
For the diagnosis of MM, quantitative real-time PCR (qPCR) was performed as described previously (24 (link), 31 (link)).
BDG was measured using a Fungitell kit (Associates of Cape Cod Inc., Falmouth, MA, USA) following the manufacturer’s instructions. The recommended cutoff of 80 pg/ml was used to determine clinical relevance.
Measurement of serum Man and GM was performed using a Platelia Candida Ag Plus test and Platelia Aspergillus Ag test (Bio-Rad, Marnes la Coquette, France), respectively, according to the manufacturer's instructions. The recommended cutoffs of a concentration of 62.5 pg/ml and an index of ≥0.5, respectively, were used.
For the diagnosis of MM, quantitative real-time PCR (qPCR) was performed as described previously (24 (link), 31 (link)).
6
Serum (1,3)-β-D-Glucan Measurement
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BDG in serum was measured using the Fungitell® kit (Associates of Cape Cod Inc., Falmouth, MA, USA), following the manufacturer’s instructions. The recommended cutoff value of 80 pg/ml was used to define positivity. Samples with BDG levels >500 pg/ml were diluted and retested.
7
Assay for CSF beta-D-glucan (BDG)
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The assay for CSF beta-D-glucan (BDG) relied on the Fungitell kit (Associates of Cape Cod, Falmouth, MA, USA). The BDG data included here, for comparative purposes with present results on the same specimens, were previously described [7 (link)], in a coccidioidal meningitis study. A positive is 31 pg/mL.
PCR. PCR data (3 samples), for comparative purposes with present results on the same specimens, was derived from a prior study [11 (link)].
PCR. PCR data (3 samples), for comparative purposes with present results on the same specimens, was derived from a prior study [11 (link)].
8
Fungitell and Platelia Candida Ag Plus Assay
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The BDG levels were determined by using the Fungitell kit (Associates of Cape Cod Inc., Falmouth, MA, USA) according to the manufacturer's instructions. The recommended cut-off of 80 pg/ml was used to determine clinical relevance. Measurement of serum Mnn levels was performed by using the Platelia Candida Ag Plus test (Bio-Rad, Marnes la Coquette, France) according to the manufacturer's instructions. The recommended cut-off was used to determine clinical relevance. For both tests, serum samples with positive results of >500pg/ml were diluted and tested again.
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