Clark type polarographic oxygen electrode

Manufactured by Hansatech

Sourced in United Kingdom

The Clark-type polarographic oxygen electrode is a device used for the measurement of dissolved oxygen concentrations in liquids. It operates on the principle of electrochemical reduction of oxygen, providing a direct electrical signal proportional to the partial pressure of oxygen in the sample.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Clark-type oxygen electrode (132 citations) Clark-type electrode (58 citations) Clark electrode (25 citations) Oxygen electrode (22 citations) Clark oxygen electrode (18 citations)

3 protocols using clark type polarographic oxygen electrode

Analyzing Mitochondrial Function and OXPHOS Complexes

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Endogenous cell respiration was assayed in whole cells in the presence of galactose using a Clark-type polarographic oxygen electrode from Hansatech Instruments (Norfolk, UK) at 30°C as described (12 (link)).
Mitochondria were prepared from the different strains as described (13 (link)) and used for spectrophotometric assays performed at 24°C to measure KCN-sensitive COX activity and antimycin A-sensitive NADH cytochrome c reductase, as described (12 (link)).
The abundance of OXPHOS complexes in mitochondrial extracts obtained in the presence of 1% lauryl-maltoside was analyzed by Blue Native polyacrylamide gel electrophoresis (BN-PAGE) using a linear 3–12% acrylamide gradient gel (14 ).

Mays J.N., Camacho-Villasana Y., Garcia-Villegas R., Perez-Martinez X., Barrientos A, & Fontanesi F. (2019). The mitoribosome-specific protein mS38 is preferentially required for synthesis of cytochrome c oxidase subunits. Nucleic Acids Research, 47(11), 5746-5760.

+ Open protocol

+ Expand

Endogenous KCN-sensitive Cell Respiration

Check if the same lab product or an alternative is used in the 5 most similar protocols

Endogenous potassium cyanide (KCN)-sensitive cell respiration was assayed in whole cells in the presence of galactose using a Clark-type polarographic oxygen electrode from Hansatech Instruments (Norfolk, UK) at 24 °C as described (Barrientos et al., 2002 (link)).

Zeng R., Smith E, & Barrientos A. (2018). Assembly of the yeast mitoribosome large subunit proceeds by the hierarchical incorporation of protein clusters and modules on the inner membrane. Cell metabolism, 27(3), 645-656.e7.

+ Open protocol

+ Expand

Quantifying Mitochondrial Function and OXPHOS

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Endogenous cell respiration was assayed in whole cells in the presence of galactose using a Clark-type polarographic oxygen electrode from Hansatech Instruments (Norfolk, UK) at 24°C as described (32 (link)).
Mitochondria were prepared from the different strains as described (6 (link)) and used for spectrophotometric assays performed at 24°C to measure KCN-sensitive COX activity, antimycin A-sensitive NADH cytochrome c reductase, and succinate cytochrome c reductase activities and oligomycin-sensitive ATP synthase activity, as described (32 (link)). Total mitochondrial cytochrome spectra were obtained as reported (32 (link)).
The abundance of OXPHOS complexes was analyzed by Blue Native polyacrylamide gel electrophoresis (BN-PAGE) using a linear 3–12% gradient gel (33 ).

De Silva D., Poliquin S., Zeng R., Zamudio-Ochoa A., Marrero N., Perez-Martinez X., Fontanesi F, & Barrientos A. (2017). The DEAD-box helicase Mss116 plays distinct roles in mitochondrial ribogenesis and mRNA-specific translation. Nucleic Acids Research, 45(11), 6628-6643.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!