V 770 uv visible absorbance spectrophotometer

The characterization of encapsulated apigenin was assessed by Transmission Electron Microscopy (TEM) (JEOL 2100, Ltd., Tokyo, Japan) operating at an accelerating voltage of 200 kV, Scanning Electron Microscope (SEM) (JEOL, JSM–IT 100), and mages were processed by using the software SEM/JSM 5000 and UV–vis spectroscopy (Jasco V-770 UV Visible Absorbance Spectrophotometer. Zeta Potential was determined by Brookhaven using a Zeta Nano Sizer and Zeta Potential. Fourier Transform Infrared Spectroscopy (FTIR) JASCO, Tokyo, Japan, model no. AUP1200343) was used to detect the surface molecular structures in the range of 500–4000 cm⁻¹ by using the KBr pellet method. X-ray diffraction was detected by Shimadzu (XRD 6100) diffractometer with CuKa1 radiation (k = 1.54056 A°).

UV-vis spectroscopy was used to assess the wavelength and to identify the specific absorption for MOLE, BSA and Encap. For MOLE, 1 mL of the samples was added to 3 mL of distilled water, which was then measured using the UV-Vis spectrophotometer (Jasco V-770 UV Visible Absorbance Spectrophotometer) in the 200–800 nm wavelength range. Additionally, the fluorescence spectrophotometer was used for the same procedure. The obtained results were analyzed utilizing Origin 8.