Human cervical cancer cell pellet, supernatant, and medium blank samples were analyzed by high‐performance liquid chromatography‐positive electrospray ionization‐tandem mass spectrometry (HPLC‐(ESI+)‐MS/MS; Shimadzu LC‐10ADvp HPLC (Columbia, MD, USA) coupled with a QTrap 5500 mass spectrometer (Sciex Applied Biosystem, Concord, ON, Canada) with a turbo V‐spray ionization source. HPLC‐(ESI+)‐MS/MS methods and multiple reaction monitoring (MRM) channels, specific for each CK, were carried out as described by Farrow and Emery.34 A 20 µL sample aliquot was injected on a reversed‐phase C18 column (Kinetex 2.6u C18 100 A, 2.1 × 50mm ; Phenomenex, Torrance, CA), and CKs were eluted with an increasing gradient of solvent B (0.08% CH3CO2H in C2H3N) mixed with solvent A (0.08% CH3CO2H in H2O), at a flow rate of 0.4 mL/min. The HPLC method for CK separation involved a multistep gradient as follows: starting conditions were 5% solution B, which increased linearly to 10% over a 2‐minute window; over the following 6.5 minutes, solution B increased to 95% and was held constant for 1.5 minutes before returning back to starting conditions for 5 minutes of column re‐equilibration.
Lc 10advp hplc
Manufactured by Shimadzu
Sourced in Japan
The Shimadzu LC-10ADvp is a high-performance liquid chromatography (HPLC) system. It is designed to perform liquid chromatography analysis with high precision and reliability. The LC-10ADvp features a solvent delivery unit, an autosampler, and a UV-Vis detector.
Automatically generated - may contain errors
4 protocols using lc 10advp hplc
1
HPLC-MS/MS Analysis of Cervical Cancer Cells
2
Quantifying PFOS and OS in Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A nitrogen evaporator was used to remove the organic solvents in the samples, and the residues were dissolved in methanol to reach constant volume. All PFOS and OS concentrations were measured using external standard method, and their calibration curves are shown in Figure S17 . High PFOS concentrations (>1 mg/L) and OS concentration were determined by a LC-10ADvp HPLC with a CDD-6A conductivity detector from Shimadzu (Japan). A mixture of methanol/0.02 mol/L NaH2PO4 solution (70/30, v/v) was selected as the mobile phase, and the limit of detection (LOD) was 0.149 mg/L for PFOS and 0.033 mg/L for OS. Low PFOS concentrations (<1 mg/L) were determined by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) with an UltiMate 3000 HPLC (Dionex by Thermo Fisher Scientific Inc., MA, USA) equipped with an API 3200 triple quadrupole mass spectrometer (AB SCIEX, ON, Canada). The mobile phase consisted of a binary mixture of 10 mmol/L ammonium acetate and methanol with a total flow rate of 0.3 mL/min. The LOD was measured to be 0.112 μg/L.
3
HPLC Quantification of Suspected Pesticides
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Following the cleaning of extract, aliquots of the final solution were quantified using a (Shimadzu) LC-10 ADvp HPLC, equipped with an SPD-M 10 Avp attached to a photodiode array detector (Shimadzu SPD-M 10 Avp, Japan) (200–800 nm). The analytical column was a C18 Reverse Phase from Alltech (250 × 4.6 mm, 5 μm) that was maintained at 30°C in a column oven. A combination of 70% ACN and 30% water was used as the mobile phase, running with a flow rate of 1.0 mL/min. All solvents were of HPLC grade and were filtered using a cellulose filter (0.45 μm) prior to use.
Prior to HPLC analysis, the samples were passed through a 0.45 μm nylon syringe filter (Alltech Assoc) before being manually injected (20 μL) each time. Suspected pesticides were identified based on the retention times of the pure analytical standards. Quantification was performed based on the method described by [28 ] (Figure 1 ).
Prior to HPLC analysis, the samples were passed through a 0.45 μm nylon syringe filter (Alltech Assoc) before being manually injected (20 μL) each time. Suspected pesticides were identified based on the retention times of the pure analytical standards. Quantification was performed based on the method described by [28 ] (
4
PK Assay for WX-554 in Plasma
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The concentration of WX-554 in plasma was measured in all patients in cycle 1 on day 1 and day 8 at 0, 1, 2, 4, 6, 8, 10 and 24 hours, and before dosing on day 1 of each subsequent cycle. Measurement was by an LC-MS/MS method developed and validated by Wilex AG (Munich, Germany) on an API 3200 with Shimadzu LC-10ADvp HPLC. After solid phase extraction of 60 l plasma on an Oasis WCX plate and elution with 2 x 250 l 2% formic acid in methanol, the samples were evaporated to dryness and re-suspended in 100 µl acetonitrile/methanol (25/75). A 10 l volume (equivalent of 6 l plasma) was injected onto an Atlantis HILIC, 2.1 x 50mm, 3 m column (Waters, Eschborn, Germany). Mobile phase was: A, 10mM ammonium formate, pH3.8/acetonitrile (95/5 v/v) and B, 10mM ammonium formate, pH3.8/acetonitrile (5/95 v/v). Elution occurred over a 2 minute gradient from 10:90 A:B to 60:40 A:B. WX-554 was eluted at 1.5 minutes. Upper and lower limits of quantification were 1000 and 1 ng/ml respectively. Both intra-assay and inter-assay coefficient of variation (CV) were <4% for all QC samples analysed.
Pharmacokinetic parameters were calculated from drug concentrations in plasma vs. time curves using Phoenix WinNonlin version 6.2 (Certara, Princeton, USA) non-compartmental analysis.
Pharmacokinetic parameters were calculated from drug concentrations in plasma vs. time curves using Phoenix WinNonlin version 6.2 (Certara, Princeton, USA) non-compartmental analysis.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!