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Cd5 alexafluor 700

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The CD5 Alexa Fluor 700 is a fluorescent-conjugated antibody used in flow cytometry analysis. It binds to the CD5 cell surface antigen, which is expressed on T cells and a subset of B cells. The Alexa Fluor 700 dye attached to the antibody emits light in the near-infrared spectrum, allowing for multicolor flow cytometry experiments.

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Lab products found in correlation

Facs lyse solution, by BD (1 mentions) Cd14 allophycocyanin, by BD (1 mentions) Recombinant human il 4, by R&D Systems (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Phosphate buffered saline (pbs), by Merck Group (1 mentions) Methanol, by Merck Group (1 mentions) Donkey anti human igm, by Jackson ImmunoResearch (1 mentions) Lsr fortessa cytometer, by BD (1 mentions) Prism 6, by GraphPad (1 mentions) Cd4 apc, by BD (1 mentions) Cd3 apc h7, by BD (1 mentions) Cd8 v500, by BD (1 mentions) Cd8 v450 rpa t8, by BD (1 mentions) Cd21 pe, by BD (1 mentions) Cd27 v450, by BD (1 mentions) Cd38 pe cy7, by BD (1 mentions) Cd45ra alexa fluor 700, by BD (1 mentions)

2 protocols using cd5 alexafluor 700

1

Multiparameter Flow Cytometry Analysis

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Antibodies obtained from BD Biosciences (San Jose, CA) include mouse anti-human CD19 peridinin-chlorophyll protein, CD5 alexafluor 700, CD20 phycoerythrin cyanine 7, CD14 allophycocyanin, CD3 phycoerythrin cyanine fluorescent 594, STAT6 tyrosine 641 phycoerythrin, and pAKTS473 phycoerythrin cyanine fluorescent 594. SYKY525/526 phycoerythrin and ERKY204 allophycocyanin were obtained from Cell Signaling Technologies (Danvers, MA). Goat anti-human IgD (Bethyl Laboratories, Montgomery, TX), donkey anti-human IgM (Jackson Immunoresearch, (West Grove, PA) and recombinant human IL-4 (R&D Systems, Minneapolis, MN) were used for whole blood stimulations. Phosphate buffered saline (PBS), bovine serum albumin (BSA), and methanol were obtained from Sigma-Aldrich (St. Louis, MO). FACS/Lyse solution was obtained from BD Biosciences.
Blunt M.D., Koehrer S., Dobson R., Larrayoz M., Wilmore S., Hayman A., Parnell J., Smith L., Davies A., Johnson P.W., Conley P.B., Pandey A., Strefford J.C., Stevenson F.K., Packham G., Forconi F., Coffey G., Burger J.A, & Steele A.J. (2016). The dual Syk/JAK inhibitor cerdulatinib antagonises B-cell receptor and microenvironmental signaling in chronic lymphocytic leukemia. Clinical cancer research : an official journal of the American Association for Cancer Research, 23(9), 2313-2324.
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2

Multiparametric Flow Cytometry of Immune Cell Subsets

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Healthy donor and patient PBMCs were obtained by using Ficoll density centrifugation and stained for 30 minutes at 48C with the following mouse anti-human antibodies: CXCR5-allophycocyanin (APC; 51505) from R&D Systems (Minneapolis, Minn); CCR7-phycoerythrin (PE)-CF594 (150503), CD27 V450 (M-T271), CD27 Brilliant Violet (M-T271), CD38-PE-Cy7 (HIT2), CD21-PE (B-ly4), CD3-APC-H7 (SK7), CD5-Alexa Fluor 700 (UCHT), CD4-APC (RPA-T4), CD4-Brilliant Violet 605 (RPA-T4), CD45RA-Alexa Fluor 700 (HI100), CD8-V500 (RPA-T8), CD8-fluorescein isothiocyanate (FITC; HIT8a), IgM-APC (G20-1273), IgD-FITC (IA6-2), and CD95-PE-Cy7 (DX2) from BD PharMingen (San Jose, Calif); CD19-peridinin-chlorophyll-protein complex-Cy5.5 (HIB19), CD10-APC (CB-CALLA), and CD31-APC (WM59) from eBioscience (San Diego, Calif); CD14-PE-Cy7 (RMO52), CD56-PE (N901), CD4-PE (13B8.2), T-cell receptor (TCR)-Vb11-FITC (C21), and TCR-Va24-PC-7 (C15) from Beckman Coulter; and CD8-V450 (RPA-T8) from BD Horizon (BD Biosciences, Franklin Lakes, NJ). Stained cells were analyzed on a BD LSR Fortessa cytometer. Data were processed with FlowJo X software (TreeStar, Ashland, Ore) and sketched with Prism 6.0 software (GraphPad Software, La Jolla, Calif).
Pfajfer L., Mair N.K., Jiménez-Heredia R., Genel F., Gulez N., Ardeniz Ö., Hoeger B., Bal S.K., Madritsch C., Kalinichenko A., Chandra Ardy R., Gerçeker B., Rey-Barroso J., Ijspeert H., Tangye S.G., Simonitsch-Klupp I., Huppa J.B., van der Burg M., Dupré L, & Boztug K. (2018). Mutations affecting the actin regulator WD repeat-containing protein 1 lead to aberrant lymphoid immunity. The Journal of allergy and clinical immunology, 142(5).
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