Fmoc ala oh

Fmoc-Phe-OH, Fmoc-Pro-OH, Fmoc-His(Trt)-OH, Fmoc-Cys(Trt)-OH, Fmoc-Asn(Trt)-OH, Fmoc-Lys(Boc)-OH, Fmoc-Ala-OH, Rink amide-AM resin, 1-hydroxybenzotriazole, benzotriazole-1-yl-oxytripyrrolidinophosphonium hexafluorophosphate, and N-methylmorpholine were obtained from GL Biochem (Shanghai, China). Dimethylformamide, methanol, dichloromethane, and n-hexane were obtained from Beijing Chemical Factory (Beijing, China). Pluronic® F-127 and DMSO were obtained from Sigma-Aldrich (St. Louis, MO, USA). Dess–Martin periodinane was provided by Aladdin (Shanghai, China). DMEM medium was purchased from Thermo Fisher Scientific (Shanghai, China). Fetal bovine serum was purchased from Kang Yuan Biology (Tianjin, China). Trypsin and Hoechst 33258 nuclear dye were obtained from Solarbio (Beijing, China). Thiazolyl blue tetrazolium bromide (MTT) was purchased from Genview (Beijing, China). WST-1 kit and Lysotraker Green procured from Beyotime (Shanghai, China). Annexin V-FITC/PI apoptosis detection kit was purchased from BestBio (Shanghai, China).

PL-Wang resin (0.4 mmole/g) was obtained from Varian, Inc. (Shropshire, UK). Fmoc-Ser(tBu)-OH, Fmoc-Met-OH, Fmoc-Leu-OH, Fmoc-Ile-OH, Fmoc-Ala-OH, Fmoc-Lys(Boc)-OH, Fmoc-Asp(OtBu)-OH, Fmoc-Asn(trt)-OH, Fmoc-Glu(OtBu)-OH, Fmoc-Phe-OH, Fmoc-Gln(trt)-OH, Fmoc-Tyr(tBu)-OH, Fmoc-Arg(pbf)-OH, N,N′-diisopropylcarbodiimide (DIC), 4-dimethylaminopyridine (DMAP) and triisopropylsilane (TIS) were obtained from GL Biochem Ltd. (Shanghai, China). Fmoc-Val-OH, Fmoc-Gly-OH, Fmoc-His(trt)-OH and N,N,N′,N′-tetramethyl-uronium-hexafluoro-phosphate (HBTU) were obtained from CS Bio Co. (Menlo Park, USA). Dimethylformamide (DMF), dichloromethane (DCM) and dimethyl sulfoxide (DMSO) were obtained from Daejung Chemicals & Metals Co., Ltd. (Siheung, Korea). Anisole, piperidine and N,N-diisopropylethylamine (DIEA) were obtained from Sigma-Aldrich (St. Louis, USA). Trifluoroacetic acid (TFA) was obtained from Yakuri Pure Chemicals Co., Ltd. (Kyoto, Japan). Ether, Anhydrous was obtained from J.T. Baker (Center Valley, USA). Zoletil® (Virbac) and Rompun® (Bayer Parma) were obtained from SMP animal medicine (Suwon, Korea). Protease inhibitor cocktail was obtained from Roche Diagnostics (IN, USA). EDTA treated BD vacutainer® was obtained from Becton, Dickinson and Company (NJ, USA). Human Aβ42 Ultrasensitive ELISA kit was obtained from Invitrogen (CA, USA).