Lox tdtomato

Wild-type C57BL/6J (000664), Glp1r-ires-Cre (029283), Gpr65-ires-Cre (029282), Npy2r-ires-Cre (029285), P2ry1-ires-Cre (029284), Agtr1a-Cre (030553), Calb2-ires-Cre (010774), Nts-Cre (017525), Vglut2-ires-Cre (016963), Sst-ires-Cre (013044), Twist2-Cre (008712), Vip-ires-Cre (010908), Piezo2-GFP-ires-Cre (027719), Trpv1-Cre (017769), Pvalb-Cre (017320), Vglut1-ires2-Cre (023527), Chat-ires-Cre (031661), lox-ChR2 (024109), lox-tdTomato (007914), and Snap25–2A-GCaMP6s-D (025111) were from the Jackson Laboratory. Drd2-Cre (032108-UCD) mice were from the Mutant Mouse Resource and Research Center (MMRRC). lox-L10-GFP mice were described before^{11 (link),12 (link)}.

All animal procedures complied with institutional animal care and use committee guidelines. P2ry1-ires-Cre, Npy2r-ires-Cre, and Gpr65-ires-Cre were prepared using standard bacterial artificial chromosome (BAC) recombineering approaches (Figure S1), as previously described (Krashes et al., 2014 (link)) (see Extended Experimental Procedures). Chat-ires-Cre (006410), Chat-GFP (007902), lox-ChR2 (012569), and lox-tdTomato (007914) were purchased (Jackson). Vglut2-ires-Cre and lox-L10-GFP mice (Krashes et al., 2014 (link); Vong et al., 2011 (link)) were generous gifts from Bradford Lowell (Beth Israel Deaconess Medical Center). All Cre driver lines used are viable and fertile and abnormal phenotypes were not detected.