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P jak1 tyr1022 1023

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P-JAK1 (Tyr1022/1023) is a lab equipment product that detects phosphorylation of Janus Kinase 1 (JAK1) at tyrosine residues 1022 and 1023. This product is used to measure the activation state of JAK1, a key signaling protein involved in various cellular processes.

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Lab products found in correlation

P jak2 tyr1007 1008, by Cell Signaling Technology (3 mentions) P tyk2 tyr1054 1055, by Cell Signaling Technology (3 mentions) C myc, by Cell Signaling Technology (2 mentions) Bcl xl, by Cell Signaling Technology (2 mentions) Ptyr705 stat3, by Cell Signaling Technology (2 mentions) P jak, by Cell Signaling Technology (1 mentions) P tyk2, by Cell Signaling Technology (1 mentions) P nf κb p65 ser536, by Cell Signaling Technology (1 mentions) P ikkα β ser176 180, by Cell Signaling Technology (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Actin, by Cell Signaling Technology (1 mentions) Pstat1 tyr701, by Cell Signaling Technology (1 mentions) P stat5 tyr694, by Cell Signaling Technology (1 mentions) Alexa fluor 488 donkey anti mouse igg antibody, by Thermo Fisher Scientific (1 mentions) Antibodies against tubulin, by Santa Cruz Biotechnology (1 mentions) Stattic, by Selleck Chemicals (1 mentions) P stat3 tyr705, by Cell Signaling Technology (1 mentions) P stat3 ser727, by Cell Signaling Technology (1 mentions) Sodium orthovanadate, by Merck Group (1 mentions) Protease inhibitors and phosphatase inhibitors, by Merck Group (1 mentions)

4 protocols using p jak1 tyr1022 1023

1

Signaling Pathway Antibody Analysis

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Antibodies against STAT3, p-STAT3 (Tyr705), JAK1, JAK2, JAK3, TYK2, Bcl-xl, C-Myc, CyclinD1, p-JAK1 (Tyr1022/1023), p-JAK2 (Tyr1007/1008), p-TYK2 (Tyr1054/1055), p-JAK(Tyr980/981), p-IKK-α/β(Ser176/180), p-NF-κB p65(Ser536)(93H1), p-Akt (Ser473) (D9E) XP®, p-GSK-3β(Ser9)(D85E12), p-p38 (Thr180/Tyr182), p-JNK (Thr183/Tyr185), and β-Actin were all obtained from Cell Signaling Technology (Danvers, MA, USA). Obtained recombinant human IL6 (Cat. 216-16) was from PeproTech. Protease and phosphatase inhibitors A and B were purchased from Millipore (Billerica, MA, USA). Bioactive drugs and compounds used for high-throughput screening were provided by TargetMol (Shanghai, China). Aldisine derivatives were obtained as mentioned in the chemical part.
Wang D.P., Wu L.H., Li R., He N., Zhang Q.Y., Zhao C.Y, & Jiang T. (2023). A Novel Aldisine Derivative Exhibits Potential Antitumor Effects by Targeting JAK/STAT3 Signaling. Marine Drugs, 21(4), 218.
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2

Screening Chinese Herb Compound Library

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The Chinese medicinal herb compound library was a collection of 182 natural compounds isolated from 69 traditional Chinese medicinal herbs (unpublished results). The compounds were dissolved in DMSO at a concentration of 10 mM. The final concentration of a compound in the screening assay was 10 μM. The library screening was performed using a cellular luciferase gene reporter assay as described below. EB (>98% purity) was purchased from Shanghai Boylechem Co.ltD. Stattic was purchased from Selleck Chemicals. Sodium orthovanadate and DAPI (Diamidino-phenyl-indole) were purchased from SIGMA. Antibodies against STAT3, p-STAT3 Tyr705, p-STAT3 Ser727, STAT1, p-STAT1 Tyr701, STAT5, p-STAT5 Tyr694, JAK2, p-JAK2 Tyr1007/1008, JAK1, p-JAK1 Tyr1022/1023, Tyk2, p-Tyk2 Tyr1054/1055, PARP and actin were purchased from CST (Cell Signaling Technology). Antibodies against tubulin were purchased from Santa Cruz Biotechnology. Secondary HRP-conjugated antibody was purchased from MultiSciences Biotech. Alexa Fluor 488 donkey anti-mouse IgG antibody was purchased from Invitrogen.
Yu X., He L., Cao P, & Yu Q. (2015). Eriocalyxin B Inhibits STAT3 Signaling by Covalently Targeting STAT3 and Blocking Phosphorylation and Activation of STAT3. PLoS ONE, 10(5), e0128406.
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3

Therapeutic Antibodies and Mouse Model

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Antibodies against p-Tyr701-STAT1 (product #9167), p-Tyr690-STAT2 (product #88410), p-Tyr705-STAT3 (product #9145), p-Tyr1022/1023-JAK1 (product #3331), p-Tyr1007/1008-JAK2 (product #3776), and p-Tyr1054/1055-Tyk2 (product #9321) were purchased from Cell Signaling Technology, USA. Recombinant human STAT3 was obtained from Abcam, UK (product #ab268982). Protease inhibitors and phosphatase inhibitors were obtained from Merck Life Science, USA. Marine natural products library was built in house in the Laboratory for Marine Resources Biology at Ocean University of China.
Six-week old female NRMI congenitally athymic nude (nu/nu) mice (SPF degree, 17–20 g weight) were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd.
Li R., Zhou Y., Zhang X., Yang L., Liu J., Wightman S.M., Lv L., Liu Z., Wang C.Y, & Zhao C. (2023). Identification of marine natural product Pretrichodermamide B as a STAT3 inhibitor for efficient anticancer therapy. Marine Life Science & Technology, 5(1), 94-101.
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4

Signaling Pathway Antibody Panel for JAK-STAT Analysis

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Antibodies against p-Tyr1022/1023-JAK1 (#3331), p-Tyr1007/1008-JAK2 (#3776), p-Tyr980/981-JAK3 (#5031), p-Tyr1054/1055-TYK2 (#9321), p-Tyr701-STAT1 (#9167), p-Tyr705-STAT3 (#9145), p-Tyr694-STAT5 (#9359), JAK1 (#3332S), JAK2 (#3230), JAK3 (#8863), TYK2 (#9312), STAT1 (#14994), STAT3 (#12640), STAT5 (#25656), c-Myc (#13987), cyclin D1 (#AF0931), Bcl-xL (#2764), p-Ser536-NF-κB (#3033), p-Thr308-AKT (#9275), p-Ser9-GSK-3β (#5558), p-Thr183/Tyr185-SAPK/JNK (#4668), NF-κB (#8242), AKT (#4691), GSK-3β (#9832), and SAPK/JNK (#9252) were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies against α-tubulin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Protease inhibitor (B14001) and phosphatase inhibitor (B15001) were purchased from Bimake (Houston, TX, USA). Recombinant human STAT3 protein (ab268982) was obtained from Abcam (Cambridge, Britain). Recombinant human interleukin-6 (IL-6) protein (200-06) was purchased from PeproTech (Rocky Hill, CT, USA). Nitrocellulose membranes and chemiluminescent horseradish peroxidase (HRP) substrate were obtained from Millipore (Billerica, MA, USA). Gefitinib was purchased from Selleckchem (Houston, TX, USA). DAB color solution, hematoxylin solution, and eosin staining solution were purchased from Servicebio (Wuhan, China).
He N., Li L., Li R., Zhang S.Q., Wu L.H., Guan X., Zhang Q.Y., Jiang T, & Yang J.B. (2023). A Novel Ageladine A Derivative Acts as a STAT3 Inhibitor and Exhibits Potential Antitumor Effects. International Journal of Molecular Sciences, 24(10), 8859.
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