We used the following primary antibodies: mouse monoclonal anti-PV (1:2000, Sigma-Aldrich), CB (1:2000, Sigma-Aldrich), calretinin (CR; 1:10000, Millipore, Billerica, MA, USA), and glutamate decarboxylase 67 (GAD67) (1:10,000, Millipore); rat monoclonal anti-BrdU (1:100; Abcam, Cambridge, MA, USA); rabbit polyclonal anti-gamma-aminobutyric acid (GABA; 1:1000, Sigma-Aldrich) and anti-Ki67 (1: 10, Ylem, Rome, Italy), and neuropeptide Y (NPY, 1: 2000, Sigma-Aldrich); and goat polyclonal anti-DCX (1:200, Santa Cruz Biotechnology, Dallas, TX, USA). We also used the following secondary antibodies: Alexa Fluor 488 and 594 goat anti-mouse IgG (both 1:200, Life Technologies, Carlsbad, CA, USA), Cy3 goat anti-mouse IgM (1:200, Millipore), and Alexa Fluor 594 goat anti-rabbit IgG and anti-rat IgG (both 1:200, Life Technologies). Biotinylated Wisteria floribunda agglutinin (1:200, Sigma-Aldrich), followed by Alexa Fluor 488 conjugated to streptavidin (10 μg/ml), was used to label PNNs using the method described above [12 (link)].
Mouse monoclonal anti pv
Manufactured by Merck Group
Sourced in United States
The Mouse monoclonal anti-PV is a laboratory equipment product that is used for the detection and identification of the parvalbumin (PV) protein in biological samples. It is a mouse-derived monoclonal antibody that specifically binds to the PV protein, allowing for its visualization and quantification through various immunoassay techniques.
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Lab products found in correlation
Gad67, by Merck Group (1 mentions)
Anti dcx, by Santa Cruz Biotechnology (1 mentions)
Rat monoclonal anti brdu, by Abcam (1 mentions)
Alexa fluor 594 goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Digital sight ds l2, by Nikon (1 mentions)
Mouse monoclonal anti cb, by Swant (1 mentions)
Mouse monoclonal anti cr, by Swant (1 mentions)
2 protocols using mouse monoclonal anti pv
1
Immunohistochemical Labeling of Neurons
2
Quantification of GABA, PV, CB, and CR Neurons in Spinal Cord
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For quantitative analysis of GABA-expressing neurons, serial coronal sections (7 µm) from the location of the open defect (exposed area) of the lumbar cord in the SBA chicks, and from a similar location in the normal chicks, were stained with a rabbit polyclonal anti-GABA antibody (1 : 1500; Sigma). The tissues were observed under a Nikon Eclipse E800 light microscope, and images were acquired using a charge-coupled device (CCD) camera attached to the microscope (Nikon Digital Sight DS-L2). For analysis of GABAexpressing neurons, all GABA-immunopositive cells with a rounded profile in superficial dorsal horn laminae I-III were considered and counted. The total num-ber of neurons in the superficial dorsal horn on each side of the spinal cord was calculated and averaged across six cross sections per chick at each age; each group comprised six chicks of each age.
For quantitative analysis of PV-, CB-, and CR-expressing neurons, serial coronal sections were stained with mouse monoclonal anti-PV (1 : 500; Sigma), mouse monoclonal anti-CB, or mouse monoclonal anti-CR (1 : 500; SWANT) antibody, and PV-, CB-, and CR-immunopositive cells were enumerated as described above.
For quantitative analysis of PV-, CB-, and CR-expressing neurons, serial coronal sections were stained with mouse monoclonal anti-PV (1 : 500; Sigma), mouse monoclonal anti-CB, or mouse monoclonal anti-CR (1 : 500; SWANT) antibody, and PV-, CB-, and CR-immunopositive cells were enumerated as described above.
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