The following reagents and antibodies were purchased from the indicated suppliers: anti-TBK1 and anti-p-TBK1 antibodies from Abcam, Cambridge, MA, USA; anti-STING, anti-β-actin, anti-IRF3, and anti-STING antibodies from Proteintech, Wuchan, China; anti-CARMA3 and anti-p-IRF3 antibodies from ABclonal, Wuhan, China.
Anti irf3
Manufactured by Proteintech
Sourced in United States
Anti-IRF3 is a primary antibody product designed to detect the interferon regulatory factor 3 (IRF3) protein. IRF3 is a transcription factor that plays a central role in the induction of type I interferon genes in response to viral infection. This antibody can be used for various research applications, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the expression and localization of IRF3 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti β actin, by Proteintech (2 mentions)
Anti p tbk1, by Cell Signaling Technology (2 mentions)
Anti p irf3, by Cell Signaling Technology (2 mentions)
Anti tbk1, by Proteintech (2 mentions)
Anti sting, by Proteintech (1 mentions)
Anti flag, by Proteintech (1 mentions)
Anti ha, by Proteintech (1 mentions)
Anti gst, by Proteintech (1 mentions)
Coralite 488 conjugated igg, by Proteintech (1 mentions)
Anti lamin a c antibody, by Transgene (1 mentions)
Anti ikkε, by Cell Signaling Technology (1 mentions)
Anti gapdh, by Proteintech (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Sgc 7901, by National Collection of Authenticated Cell Cultures (1 mentions)
Bgc823, by National Collection of Authenticated Cell Cultures (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Anti akt, by Cell Signaling Technology (1 mentions)
Anti p akt, by Cell Signaling Technology (1 mentions)
Hrp conjugated goat anti rabbit, by Proteintech (1 mentions)
Metformin hcl, by Selleck Chemicals (1 mentions)
5 protocols using anti irf3
1
Immunoblotting Analysis of TBK1, STING, and IRF3
2
Cell Line Maintenance and Antibody Validation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human embryonic kidney cell HEK293T, hepatic carcinoma cell HepG2, human lung carcinoma cell A549, and human colorectal adenocarcinoma Caco-2 were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (HyClone, Logan, UT) containing 10% inactivated fetal bovine serum (BBI, Shanghai, China), penicillin (100 IU/ml), and streptomycin (100 mg/ml) at 37 °C in a 5% CO2 atmosphere. Sg25 cell is derived from HepG2 cell, in which TRIM25 has been knocked out through the CRISPR/Cas9 system.45 (link)Antibodies against STAT1, p-STAT1, and STAT2 were purchased from Abcam (Cambridge, MA, USA); anti-IRF3, anti-TBK1, anti-HA, anti-GST, anti-Flag, anti-GAPDH, CoraLite 594-conjugated IgG, and CoraLite 488-conjugated IgG secondary antibodies were obtained from Proteintech (Rosemont, IL, USA); anti-TRIM25, anti-IKKε, anti-pIKKε, anti-pTBK1, and anti-pIRF3 antibodies were from Cell Signaling technology (Danvers, MA, USA); anti-pSTAT2 antibody was from Sigma-Aldrich (St. Louis, MO, USA); anti-Lamin A/C antibody was from TransGen (Beijing, China); anti-SeV antibody was from MBL (Beijing, China); Human anti-SARS-CoV & CoV-2 NP antibody was from ZENBIO (Chengdu, China).
3
Investigating Gastric Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human gastric cancer cell lines BGC823, AGS, and SGC7901 were purchased from the National Collection of Authenticated Cell Cultures of China and were authenticated using Short Tandem Repeat (STR) analysis by the users’ lab. Cells were cultured in RPMI-1640 medium (Gibco, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, USA) and 1% penicillin-streptomycin in a humid incubator with 5% CO2 at 37 °C. The primary antibodies used were anti-p-TBK1 (1:1000; #5483, Cell Signaling Technology), anti-TBK1 (1:1000; #67211-1-Ig, Proteintech), anti-p-IRF3 (1:1000; #29047, Cell Signaling Technology), anti-IRF3 (1:1000; #11312-1-AP, Proteintech), anti-p-AKT (1:1000; #4060, Cell Signaling Technology), anti-AKT (1:1000; #9272, Cell Signaling Technology), anti-SOX2 (1:1000; #11064-1-AP, Proteintech), anti-STING (1:1000; #13647, Cell Signaling Technology), anti-cGAS (1:1000; #26416-1-AP, Proteintech), anti-FLAG (1:5000; #66008-4-Ig, Proteintech), and anti-GAPDH (1:3000; #60004-1-Ig, Proteintech). The secondary antibodies used were HRP-conjugated goat anti-rabbit (1:3000; #SA00001–15, Proteintech) and anti-mouse (1:3000; #SA00001–1, Proteintech). Metformin HCl (#S1950) and SC79 (#S7863) were purchased from SELLECK (TX, USA).
4
PDCoV Infection Pathway Protein Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The cells were lysed with radioimmunoprecipitation assay (RIPA) lysis buffer (Solarbio, Beijing China) containing a protease/phosphatase inhibitor cocktail (Cell Signaling Technology, MA, USA). The protein concentration was quantified using a BCA Protein Assay kit (23227, TermoFisher Scientifc, Waltham, MA, USA); the protein was separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and then transfered onto polyvinylidene fluoride membranes. After blocking the membranes with 5% skim milk at room temperature for 1.5 h and incubation at 4 °C overnight, different membranes were incubated with the following corresponding primary antibodies: anti-PDCoV N (1:1000, Medgene, South Dakota, USA), anti-RIG-I, anti-IRF3, anti-TRAF6 (1:1000, ProteinTech Group, Chicago, IL, USA), anti-NF-κB (1:1000, Cell Signaling Technology, USA) and anti-β-actin (1:1000, ProteinTech Group, USA). Horseradish peroxidase conjugated to AffiniPure goat anti-mouse IgG (1:5000, ProteinTech Group, USA) or goat anti-rabbit IgG (1:5000, ProteinTech Group, USA) was used as secondary antibodies.
5
Western Blot Analysis of Immune Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were lysed in radioimmunoprecipitation assay (RIPA) lysis buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, NP-40, 1 mM EDTA, and 1× protease inhibitor cocktail [Roche, Mannheim, Germany]) according to the manufacturer’s protocol. The samples were separated with sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis, transferred onto nitrocellulose membrane, and probed with the indicated antibodies. Where indicated, Western blot signals were quantified by densitometry as previously described (51 (link), 52 (link)). The following antibodies were used: anti-polyclonal-NP (produced in our lab), anti-phospho-IRF3 (Abcam, Cambridge, UK), anti-IRF3 (Proteintech, Wuhan, China), anti-STAT1 (Abcam), anti-phospho-STAT1 (Abcam), anti-β-actin (TranGens Biotech, Beijing, China), anti-p65 (Abclonal, Wuhan, China) and anti-phospho-p65 (Abclonal).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!