Succinic anhydride

Manufactured by J&K Scientific

Sourced in China

Succinic anhydride is a chemical compound with the molecular formula (CH2CO)2O. It is a cyclic anhydride that serves as a versatile intermediate in organic synthesis, particularly in the production of various chemicals and pharmaceuticals.

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Lab products found in correlation

7 protocols using succinic anhydride

Synthesis and Characterization of Multifunctional Polymer Conjugates

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(3-Mercaptopropyl) trimethoxysilane (97%, J&K), 2,2'-dithiodipyridine (98%, Energy Chemical), poly(ethylene glycol) methyl ether (PEG-OH, M_n = 750 g/mol, 98%, Energy Chemical), thionyl chloride (97%, J&K), 2-mercaptoethylamine hydrochloride (MCA, 99%, Energy Chemical), 1-(2-aminoethyl)piperazine (AEPZ, 97%, Aldrich), diisopropanolamine (DIAD, 98%, Energy Chemical), phthalimide (97%, Energy Chemical), triphenylphosphine (99%, Energy Chemical), hydrazine hydrate (95%, J&K), triethylamine (TEA, 99%, Beijing Chemical Works), succinic anhydride (99%, J&K), N, N'-bis(acryloyl)cystamine (BAC, 98%, Fluka), β-CD ( > 95%, TCI) was dried at 70 °C under vacuum for 24 h before use. Cell counting kit-8 was purchased from Beyotime Biotechnology. DMEM (Corning) with 4.5 g /L glucose, l-glutamine, without sodium pyruvate was purchased from Beijing BioDee Biotechnology Co. Ltd. MCF-7 cell lines were purchased from the American type Culture Collection (ATCC, Rockville, MD). Other reagents were commercially obtained from Beijing Chem. Co. and used without further purification.

Wang X., Gao P., Yang Y., Guo H, & Wu D. (2018). Dynamic and programmable morphology and size evolution via a living hierarchical self-assembly strategy. Nature Communications, 9, 2772.

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Zeb1/2 Gene Silencing Nano-Delivery

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Branched polyethyleneimine (PEI, Mw 2 kDa), 2-(N,N-diethylamino)ethyl methacrylate (DEA), Cysteamine (95%), Azobisisobutyronitrile (AIBN), Succinic anhydride (SA), cis-Aconitic anhydride, dimethyl sulfoxide (DMSO), Triethylamine (TEA), pyridine, and acetone were purchased from J&K Scientific Ltd. (Beijing, China). ZrOCl2∙8H2O, Tetrakis (4-carboxyphenyl)porphyrin (TCPP), N,N-Dimethylformamide (DMF), N-ethyl-N-(3-(dimethylamino)-propyl) carbodiimide hydrochloride (EDC), and N-hydroxysuccinimide (NHS) were supplied by Aladdin Industrial Co., Ltd. (Shanghai, China). The siZEB1 (GTTGTTCTGCCAACAGTTG) and siZEB2 (GGCCGAATGAGAAACAATA) target both human and mice’s genes, and Cy5-labeled siZEB1/2 were designed and synthesized by RiboBio (Guangzhou, China). Lipofectamine 2000 was purchased from Invitrogen (Carlsbad, United States), and the LPS was obtained from Sigma-Aldrich (Missouri, United States). The Cell Counting Kit-8 (CCK-8) was bought from Boster Biological Technology (Wuhan, China). DAPI and total RNA Reagent Trizol were purchased from Life Technologies (New York, United States). The PrimeScript®RT Reagent Kit with gDNA Eraser was provided by TaKaRa (Beijing, China). Quantitative real-time qPCR kits (iTaq™ universal SYBR^® Green Supermix) were purchased from Bio-Rad (California, United States).

Weng C., Li G., Zhang D., Duan Z., Chen K., Zhang J., Li T, & Wang J. (2022). Nanoscale Porphyrin Metal-Organic Frameworks Deliver siRNA for Alleviating Early Pulmonary Fibrosis in Acute Lung Injury. Frontiers in Bioengineering and Biotechnology, 10, 939312.

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Anhydride Synthesis and Cytokine Analysis

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Dex and succinic anhydride were purchased from J&K Scientific Ltd (Beijing, China). Glutaric anhydride was purchased from Beijing Bailingwei Technology Co., Ltd. (Beijing, China). Adpic anhydride was purchased from Nanjing Kangmanlin Biology Technology Co., Ltd. (Nanjing, China). Phthalic anhydride was purchased from Adamas-beta Co., Ltd. (Shanghai, China). Mouse TNF-α DuoSet ELISA (DY410-05) and mouse IL-6 DuoSet ELISA (DY406-05) were obtained from R&D SYSTEMS® (Minneapolis, MN, USA). All other used agents were of analytical grade.

Liu H., Yu A., Dai M., Lin D., Lin D., Xu X., Li X, & Wang Y. (2020). Effects of Terminal Motif on the Self-Assembly of Dexamethasone Derivatives. Frontiers in Chemistry, 8, 9.

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Dextran Uptake and KRASG12V Expression in BxPC-3 Cells

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Reagents were purchased from commercial sources: FITC-labeled 4 kDa, 70 kDa, 150 kDa dextrans (Sigma, USA), TMR 70 kDa-labeled dextran (Life Technologies), TP (Chengdu Purifa, China), succinic anhydride (J&K Scientific, China), DMAP (Macklin, China), CDI (Macklin, China), esterase from porcine liver (Sigma, USA), EIPA (MedChemExpress, USA), and LysoTracker (Life Technologies).
Plasmids were obtained from Addgene (USA): pBABE-Puro-KRAS^G12V was a gift from Christopher Counter and the pBABE-Puro vehicle control was a gift from Hartmut Land, Jay Morgenstern and Bob Weinberg. All plasmids were packaged into lentiviruses with RSV, VSV, and pMDL and transduced into BxPC-3 cells via infection and selected under puromycin pressure (~1.5 µg/ml).
Antibodies used for immunohistochemical staining were from Servicebio (China): anti-Ki67 (GB111141), anti-EpCAM (GB11274), anti-CD3 (GB13014), anti-CD4 (GB13064-2), anti-CD8 (GB13429), anti-CD45 (GB11066), anti-IFN-γ (GB11107-1) and anti-TNF-α (GB11188).

Yuan F., Sun M., Liu Z., Liu H., Kong W., Wang R, & Qian F. (2022). Macropinocytic dextran facilitates KRAS-targeted delivery while reducing drug-induced tumor immunity depletion in pancreatic cancer. Theranostics, 12(3), 1061-1073.

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Paclitaxel-Loaded Polymeric Nanoparticles

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Paclitaxel was purchased from Nanjing Jingzhu Bio-technology Co., Ltd. (Nanjing, China). Pyrene (purity 97%) was purchased from Shanghai Jingchun Bio-technology Co., Ltd. (Shanghai, China). Dialysis tube (MWCO 2000/350) was obtained from Shanghai Lvniao Technologies, Inc. (Shanghai, China). Glutathione (GSH), 4-(dimethylamino-pyridin (DMAP, purity > 98%), 3, 3′-Dithiodipropionic acid (DTDP, purity > 98%), Acetyl chloride (purity > 98%), CaH₂ (purity > 98.5%) and N, N'-Dicyclohexyl-carbodiimide (DCC, purity > 98%) were purchased from Aladdin Reagent Co., Ltd. (Beijing, China). Succinic anhydride (purity > 99% GC), Acetonitrile hyper grade for HPLC, DMSO-d₆, and CDCl₃ were purchased from J & K Scientific Ltd. (Beijing, China). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl Tetrazolium bromide (MTT, 5 mg/mL Sigma-Aldrich), Dulbecco's modified Eagle medium (DMEM), penicillin-streptomycin (Amresco, USA), 0.25% (w/v) trypsin-EDTA solution and fetal bovine serum (FBS, Gibco, USA) were purchased from Sigma-Aldrich. All chemicals were analytical grade and used without further treatment. The mPEG-PBLA was synthesized according to the literature (41 (link)) and dithiodipropionic anhydride was also synthesized according to previous report (42 (link)).

Chang S., Wang Y., Zhang T., Pu X., Zong L., Zhu H., Zhao L, & Feng B. (2019). Redox-Responsive Disulfide Bond-Bridged mPEG-PBLA Prodrug Micelles for Enhanced Paclitaxel Biosafety and Antitumor Efficacy. Frontiers in Oncology, 9, 823.

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Chitosan-Dopamine Hydrogel Synthesis

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Chitosan (M_n = 100 000–300 000 Da), dopamine hydrochloride (DA) (purity 98%) and succinic anhydride were obtained from J&K. Polyethylene glycol monomethyl ether (mPEG), methacrylate anhydride (94%), glycidyl methacrylate (≥97%) and zinc chloride were purchased from Sigma-Aldrich. All other reagents were used without further purification.

Yang Y., Liang Y., Chen J., Duan X, & Guo B. (2021). Mussel-inspired adhesive antioxidant antibacterial hemostatic composite hydrogel wound dressing via photo-polymerization for infected skin wound healing. Bioactive Materials, 8, 341-354.

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Synthesis of Fluorescent Polymer Conjugates

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N,N-Dimethylformamide, 9-anthracenemethanol, toluene, poly(ether diamine) (Jeffamine D230), and 4-methylbenzenesulfonic acid were purchased from Adams-Beta Co. Ltd. (Shanghai, China). 2-Mercaptoethanol and succinic anhydride were purchased from J&K Scientific Ltd. Chloroform, polybutadiene (PB) (Mw ∼ 200000), and 1,4,5,8-naphthalenetetracarboxylic dianhydride were purchased from Sigma-Aldrich. All the reagents were used as received.

Bai J., Zhang L., Hou H., Shi Z., Yin J, & Jiang X. (2019). Light-Written Reversible 3D Fluorescence and Topography Dual-Pattern with Memory and Self-Healing Abilities. Research, 2019, 2389254.

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