CD4+ T cells, Teffs or Tregs (105 cells per well) were incubated for 30 min at room temperature with BT-061, OKT-3, RPA-T4, SK3, QS4120, B-A1, MT441, MT310, EDU-2 or OKT-4 (1 μg ml−1). The mAbs were cross-linked for 5, 10, 30 or 60 min at 37 °C either by anti-human IgG (20 μg ml−1) (Life Technologies) or anti-mouse IgG (amIgG) (10 μg ml−1) (Thermo Scientific, Waltham, MA, USA). For intracellular staining, cells were treated with fixation buffer, permeabilized with perm buffer III and stained with anti-Zap-70 (pY319)/Syk (pY352), anti-PLC-γ2 (pY759), anti-ERK1/2 (pT202/pY204), anti-NF-κB p65 (pS529), anti-PKCα (pT497), anti-p38 MAPK (pT180/pY182), anti-Btk (pY551)/Itk (pY511), anti-MEK1 (pS298), anti-Pyk2 (pY402), anti-SLP-76 (pY128), anti-Lck (pY505), anti-Akt (pS473), anti-LAT (pY226), anti-JNK (pT183/pY185), anti-SHP-2 (pY542) or anti-IKKγ (pS376) according to the manufacturer's instructions (all BD Phosflow, San Jose, CA, USA) before being analyzed on a FACS Canto II. Fold induction was calculated by dividing the mean fluorescence intensity of the measured value by the mean fluorescence intensity of the untreated control.
Anti shp 2
Manufactured by BD
Sourced in United States
Anti-SHP-2 is a laboratory reagent used in research applications. It functions as an inhibitor of the SHP-2 protein, which is involved in cellular signaling pathways. The core purpose of Anti-SHP-2 is to facilitate the study of SHP-2 and its role in biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Anti erk1 2, by BD (1 mentions)
Anti akt, by BD (1 mentions)
Anti slp76, by BD (1 mentions)
Anti jnk, by BD (1 mentions)
Anti pkc α, by BD (1 mentions)
Anti p38 mapk, by BD (1 mentions)
Anti lck, by BD (1 mentions)
Anti zap 70, by BD (1 mentions)
Anti ikkγ, by BD (1 mentions)
Facscanto 2, by BD (1 mentions)
Anti btk, by BD (1 mentions)
Anti nf κb p65, by BD (1 mentions)
Anti shp2, by Santa Cruz Biotechnology (1 mentions)
Human icam 1 his, by Sino Biological (1 mentions)
Human pd l1 his, by Sino Biological (1 mentions)
Anti shp 1, by Proteintech (1 mentions)
Anti gfp, by Thermo Fisher Scientific (1 mentions)
Anti gapdh, by Proteintech (1 mentions)
2 protocols using anti shp 2
1
Quantifying T-Cell Activation Signaling
2
Immunostaining and Flow Cytometry Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Biotin anti-human CD3ε (clone OKT3, BioLegend, #317320), human PD-L1-His (Sino Biological, #10084-H08H), human ICAM-1-His (Sino Biological, #10346-H08H), and streptavidin (Invitrogen, #S888) were used to functionalize SLBs. Anti-Shp1 (Life Technologies, #3H20L13), anti-Shp2 (BD Biosciences, #101720), and F (ab') 2-Goat anti-Rabbit IgG (H+L) secondary antibody, alexa fluor 568 (Invitrogen, #A21069) were used in the immunostaining for the cell-SLB assays. Anti-Phosphotyrosine (clone pY20, Sigma-Aldrich, #P4110-1MG), anti-GFP (Invitrogen, #A-6455), Anti-Shp1 (Proteintech, #24546-1-AP), anti-Shp2 (Santa Cruz Biotechnology, #sc-7384), and Anti-GAPDH (Proteintech, #10494-1-AP) were used in western blots. Phycoerythrin (PE) anti-human BTLA (clone MIH26, BioLegend, #344505) and Pacific Blue anti-human PD-1 (clone EH12.2H7, BioLegend, #329916), were used in the staining of Jurkat or Raji cells for flow cytometry.
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